Below are links to protocols. Some protocols can be found as pdfs at the bottom of the page. As similar protocols are shared with us, we will create reference records and links.
Protocols shared by Isabel Faci-Gomez and Cristobal Uauy, John Innes Centre:
(the pdfs of these protocols can be found at the bottom of the page)
- How to grow wheat This protocol explains how to grow wheat in controlled and semi-controlled environments from germination to harvest. James Simmonds, Isabel Faci, Valentina Buffagni, Cristóbal Uauy, 2024. Journal article page: https://dx.doi.org/10.17504/protocols.io.6qpvr37jbvmk/v1 GrainGenes reference page: PTC-2024:6qp
- Wheat inflorescence meristem micro-dissection This protocol provides a method for micro-dissecting young wheat plants to access developing inflorescence (spike) meristems. Isabel Faci, Anna Elisabeth Backhaus, Cristóbal Uauy, 2024. Journal article page: https://dx.doi.org/10.17504/protocols.io.3byl49r2zgo5/v1 GrainGenes reference page: PTC-2024:3by
- Nuclei isolation from wheat meristems This protocol describes how to isolate intact and clean nuclei from wheat meristem tissues (spikes and carpels/ovaries), specifically from flash-frozen tissues. Isabel Faci, Maximillian RW Jones, Cristóbal Uauy, 2024. Journal article page: https://dx.doi.org/10.17504/protocols.io.yxmvm3renl3p/v1 GrainGenes reference page: PTC-2024:xym
- Wheat high molecular weight DNA extraction This protocol is for the extraction of high-molecular weight DNA from wheat leaf tissue, suitable for long-read sequencing (e.g, PacBio). Maximillian RW Jones, Nikolai Adamski, Cristóbal Uauy 2024. Journal article page: https://dx.doi.org/10.17504/protocols.io.bp2l6xpy1lqe/v1 GrainGenes reference page: PTC-2024:bp2
- RNA extraction from wheat stigmas This protocol provides a method for extracting high-quality RNA from wheat stigmas which have been stored in DNA/RNA shield. It is suitable for field samples and tissues with high carbohydrate content. Marina Millán Blánquez, 2022. Journal article page: https://dx.doi.org/10.17504/protocols.io.36wgq7kj3vk5/v1 GrainGenes reference page: PTC-2022:36w
MAS Wheat Protocols
Created and maintained at UC Davis by Jorge Dubcovsky and Marcelo Soria
** Please see the GG Reference page for links to genes, alleles, loci, probes, and QTL records in the GrainGenes database.
Agronomic Traits
- Plant height gene Rht25 MAS Wheat | Protocol at GG | GG Reference
- Spikelet number per spike gene FT-A2 MAS Wheat | Protocol at GG | GG Reference
- WAPO1. A candidate gene for spikelet number per spike. MAS Wheat | Protocol at GG | GG Reference
- Spikelet and spike number gene TaCol-B5. MAS Wheat | Protocol at GG | GG Reference
- Spikelet number per spike gene bZIPC1 MAS Wheat | Protocol at GG | GG Reference
- Thousand Grain Weight and Grain Size gene TaGW2-A1 MAS Wheat | Protocol at GG | GG Reference
Quality Traits
- Grain Texture / Hardness MAS Wheat | Protocol at GG | GG Reference
- High grain protein content (HGPC) / Yr36 MAS Wheat | Protocol at GG | GG Reference
- Waxy mutants MAS Wheat | Protocol at GG | GG Reference
Abiotic Stress Traits
- Reduced cadmium concentration. MAS Wheat | Protocol at GG | GG reference
Stripe Rust Resistance
- Stripe Rust Resistance. Yr5 MAS Wheat | Protocol at GG | GG Reference
- Stripe Rust Resistance. Yr15 MAS Wheat | Protocol at GG | GG Reference
- Stripe Rust Resistance. Yr16 and QTL QYr.ufs-2A MAS Wheat | Protocol at GG | GG Reference
- Stripe Rust Resistance. Yr26 MAS Wheat | Protocol at GG | GG Reference
- Stripe Rust Resistance. Yr45 MAS Wheat | Protocol at GG | GG Reference
- Stripe Rust Resistance Yr46 in Lr67/Yr46/Sr55/Pm46/Ltn3 MAS Wheat | Protocol at GG | GG Reference
- Stripe Rust Resistance. Yr48 MAS Wheat | Protocol at GG | GG Reference
- Stripe Rust Resistance. Yr51 MAS Wheat | Protocol at GG | GG Reference
- Stripe Rust Resistance. Yr59 MAS Wheat | Protocol at GG | GG Reference
- Stripe Rust Resistance. Yr60 MAS Wheat | Protocol at GG | GG Reference
- Stripe Rust Resistance. YrR61 MAS Wheat | Protocol at GG | GG Reference
- Stripe Rust Resistance. Yr78 MAS Wheat | Protocol at GG | GG Reference
Stem Rust Resistance
- Stem Rust Resistance. Sr13 MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. Sr21 MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. Sr22. MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. Sr24 MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. Sr25 MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. Sr26 MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. Sr28 MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. Sr32 MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. Sr33 MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. Sr35 MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. Sr36 MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. Sr39-Lr35 MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. Sr43 MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. Sr44 MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. Sr45 MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. Sr47 MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. Sr49. MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. Sr52 MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. Sr55 in Lr67/Yr46/Sr55/Pm46/Ltn3 MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. Sr56 MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. SrCad MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. SrND643 MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. SrTA10171. MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. SrTA10187 MAS Wheat | Protocol at GG | GG Reference
- Stem Rust Resistance. QSr.abr-7AL MAS Wheat | Protocol at GG | GG Reference
Leaf Rust Resistance
- Leaf Rust Resistance. Lr19, Sr26, Color gene Y MAS Wheat | Protocol at GG | GG Reference
- Leaf Rust Resistance. Lr21 MAS Wheat | Protocol at GG | GG Reference
- Leaf Rust Resistance. Lr22a MAS Wheat | Protocol at GG | GG Reference
- Leaf Rust Resistance. Lr25 and Lr29 MAS Wheat | Protocol at GG | GG Reference
- Leaf Rust Resistance. Lr32 MAS Wheat | Protocol at GG | GG Reference
- Leaf Rust Resistance. Lr34-Yr18 MAS Wheat | Protocol at GG | GG Reference
- Leaf Rust Resistance. Lr39 MAS Wheat | Protocol at GG | GG Reference
- Leaf Rust Resistance. Lr46 - Yr29. MAS Wheat | Protocol at GG | GG Reference
- Leaf Rust Resistance. Lr47 MAS Wheat | Protocol at GG | GG Reference
- Leaf Rust Resistance. Lr50 MAS Wheat | Protocol at GG | GG Reference
- Leaf Rust Resistance. Lr51 MAS Wheat | Protocol at GG | GG Reference
- Leaf Rust Resistance. Lr67 in Lr67/Yr46/Sr55/Pm46/Ltn3 MAS Wheat | Protocol at GG | GG Reference
- Leaf Rust Resistance. Lr68 MAS Wheat | Protocol at GG | GG Reference
