from Mauricio La Rota, November 2003
    last update February 2005

    EST-SSRs from wheat, barley and rice

    From La Rota and Sorrells, "Development of microsatellite markers that map across related species", BMC-Genomics, in press.

    Primers were designed from consensus sequences from the TIGR Gene Indices, to avoid redundancy in reporting primers from multiple ESTs from the same gene.

    • EST-SSR primers for wheat (5425), barley (3036) and rice (4726).
      Untested, for your exploration. Includes locations of the best BLAST hits in the rice genome. (.xls format, 14 MBytes)
      If you use these primers in your own work, we encourage you to make your test results available.
      You may use the report form or simply send a file to Mark Sorrells, mes12@cornell.edu.

    • Results from these primers
      • from Ju-Kyung Yu, Oct 2003
      • ...

    • Software used
      • Modified Sputnik (source code). Description of changes.
      • Perl script for batch PCR primer design with primer3.
      • Windows version of primer3 (source code).
      • Schema for the MySQL database.

    <= Triticeae EST-SSR Coordination page