TABLE OF CONTENTS TABLE OF CONTENTS TITLE PAGE 1994 COOPERATING AGENCIES AND STATIONS INTRODUCTION SOURCE OF THE 1994 CROP SAMPLES UNIFORM REGIONAL NURSERY TRIALS ENTRIES IN THE UNIFORM REGIONAL HARD RED SPRING WHEAT PERFORMANCE NURSERY METHODS DISCUSSION UNIFORM REGIONAL NURSERY SAMPLES - 1994 CROP TITLE PAGE UNITED STATES DEPARTMENT OF AGRICULTURE AGRICULTURAL RESEARCH SERVICE in cooperation with STATE AGRICULTURAL EXPERIMENT STATION QUALITY EVALUATION OF HARD RED SPRING WHEAT CULTIVARS 1994 CROP1/ by G.A. Hareland, W.J. Newell, J.G. Wear,2/, and M. Skunberg3/ ___________________________________________________________________________ 1/ This report represents cooperative investigations on the quality of Hard Red Spring Wheat cultivars from the 1994 crop. Some of the results presented have not been sufficiently confirmed to justify varietal release. Confirmed results will be published through established channels. Cooperators submitting samples for analysis have been given analytical data on their samples prior to release of this report. This report is primarily a tool for use by cooperators and their official staff and to those individuals having direct and special interest in the development of agricultural research programs. This report was compiled by the Agricultural Research Service, U. S. Department of Agriculture. Special acknowledgment is made to the North Dakota State University for use of their facilities and the services provided in support of these studies. The report is not intended for publication and should not be referenced in either literature citations or quoted in publicity and advertising. Use of the data may be granted for certain purposes upon written request to the agency or agencies involved. 2/ Research Food Technologist and Physical Science Technicians, USDA/ARS Hard Red Spring & Durum Wheat Quality Laboratory, NDSU, Fargo, ND. 3/ Food Technologist, Dept. of Cereal Science & Food Technology, NDSU, Fargo, ND. 1994 COOPERATING AGENCIES AND STATIONS The cooperative agencies and stations conducting the varietal plot and nursery experiments from which the 1994 spring wheat samples were received are listed below: Idaho Agricultural Experiment Station Aberdeen, Tetonia Minnesota Agricultural Experiment Station Morris, St. Paul Montana Agricultural Experiment Station Bozeman, Havre, Sidney North Dakota Agricultural Experiment Station Minot, Langdon, Dickinson, Casselton, Carrington, Prosper, Williston South Dakota Agricultural Experiment Station Brookings, Groton, Selby Washington Agricultural Experiment Station Pullman Wisconsin Agricultural Experiment Station Madison Wyoming Agricultural Experiment Station Powell A complete list of all cooperating agencies, stations, and personnel for the year will be found in the report by R. H. Busch, et al., Wheat Varieties Grown in Cooperative Plot and Nursery Experiments in the Spring Wheat Region in 1994.4/ _____________________________________________________________________________ 4/ Busch, R. H. Wheat Varieties Grown in Cooperative Plot and Nursery Experiments in the Spring Wheat Region in 1994. Agricultural Research Service, U. S. Department of Agriculture and State Agricultural Experiment Station, St. Paul, MN. INTRODUCTION Samples of standard cultivars and new selections of hard red spring wheat grown in cooperative experiments in spring wheat regions of the United States are milled each year by the USDA/ARS, Wheat Quality Laboratory. Wheat and their corresponding flours are evaluated for physical and chemical properties, and the flours are baked to determine bread characteristics. The purpose of this report is to make available to the cooperators and other interested parties, quality data on the standard varieties and new selections of hard red spring wheat from the 1994 crop. The evaluation of a wheat sample involves the analysis of kernel characteristics, milling performance, and baking performance. A brief description of testing methods employed is shown on pages 9-12. No specific comments are made regarding mixogram patterns derived from samples. However, reference mixograms, shown on page 17, illustrate ranges from which sample mixograms may be compared. SOURCE OF THE 1994 CROP SAMPLES Tests were performed on 1211 samples which were received from 24 stations in 10 states. However, data on 625 samples is excluded from this report, because the information was of interest only to plant breeders at specific experiment stations. Data presented in this report represents the evaluation of spring wheats received from Field Plot Nurseries and Uniform Regional Nurseries. The following stations were cooperators: Idaho: Aberdeen, Tetonia Minnesota: Morris and St. Paul Montana: Bozeman, Havre, Sidney North Dakota: Minot, Langdon, Dickinson, Casselton, Williston, Carrington, Prosper South Dakota: Brookings, Groton, Selby Washington: Pullman Wisconsin: Madison Wyoming: Powell UNIFORM REGIONAL NURSERY TRIALS The geographical areas from which the samples were received are shown on page 7. Spring wheat cultivars and experimental lines included in the Uniform Regional Nursery trials are listed on page 8. The Northeast area included 4 stations; the Southeast area included 6 stations; the Midwest area included 4 stations; and the West area included 5 stations. ENTRIES IN THE UNIFORM REGIONAL HARD RED SPRING WHEAT PERFORMANCE NURSERY The 32 entries in the 1994 URHRSWPN are listed below: Entry Cross or CI No. or Year No. Variety Selection No. Entered Source 1. Marquis 3561 1929 Canada 2. Chris 13751 1969 USDA-MN 3. Era 13986 1972 USDA-MN 4. Stoa 1987 ND 5. Butte 86 1987 ND 6. SD0007 MN7663/SBY354A 1994 SD 7. SD0010 YW352/SBZ004A 1993 SD 8. SD0014 YW352/SBZ004A 1994 SD 9. SD3151 SD3036//SD8052/SD2971 1994 SD 10. SD3156 Butte 86/SD8061 1994 SD 11. SBE0437 MN7663/SBY354A 1993 USDA-MN 12. MN90138 MN86400/MN86033 1994 USDA-MN 13. MN91227 MN87003/MN86497 1994 USDA-MN 14. MN91309 SBI0037 1994 USDA-MN 15. MN91324 SBI0072 1994 USDA-MN 16. MN92006 Marshall/MN88076 1994 USDA-MN 17. SBE0050 W8814/Norak 1994 USDA-MN 18. ND673 Grandin/Stoa 'S' 1992 ND 19. ND674 Grandin*2/ND6453 1993 ND 20. ND677* ND622*2/Cutless 1993 ND 21. ND678 Stoa'S'/3/IAS20*4/H567.71 1993 ND //Amidon 22. ND686 Grandin'S'/3/Colonia 1994 ND S/Amidon//Grand 23. N90-0671 Bergen/N86-0111 1993 AGPRO 24. N90-0666 Bergen/N86-0111 1993 AGPRO 25. N90-0700 Prospect/Amidon 1993 AGPRO 26. N89-0562 CNO'S'/7C/3/B.RE//BA 1994 AGPRO PET/ANB 27. N91-0059 HS85-510/Success 1994 AGPRO 28. N90-0392 HS85-0476/HS84-0700 1994 AGPRO 29. N91-3057 B85-1525/Krona 1994 AGPRO 30. BW688 BW608/Norseman 1994 AGCAN 31. BW173 COL*2//Saric/NEP/3/COL*5//Saric/NEP 1994 AGCAN 32. BW174 COL*2//Saric/NEP/3/COL*5//Saric/NEP 1994 AGCAN * Solid stem lines for sawfly resistance, METHODS Following are the variables and testing methods used in the evaluation process: Test Weight Per Bushel - The weight per Winchester bushel of cleaned, dry wheat subsequent to passing the sample through a Carter-Day dockage tester. 1000-Kernel Weight - The weight of 1000 kernels was determined by counting, using a Seedburo seed counter, the number of kernels in 10 g samples of cleaned, hand- picked wheat.5/ Kernel Size - The percentages of the size of kernels (large, medium and small) were determined using a wheat sizer as described by Shuey6/. The sieves of the sizer were clothed as follows: Top Sieve - Tyler #7 with 2.92 mm opening Middle Sieve - Tyler #9 with 2.24 mm opening Bottom Sieve - Tyler #12 with 1.65 mm opening Milling - The samples were cleaned by passing the wheat through a Carter-Day dockage tester and through a modified Forster scourer (Model 6). The clean, dry samples were pretempered to 12.5% moisture for at least 72 hours, then tempered to 15.5% moisture and allowed to stand overnight prior to milling. ____________________________________________________________________________ 5/ Mention of a trademark name or a proprietary product does not constitute a guarantee or warranty of the product by the U. S. Department of Agriculture, and does not imply its approval to the exclusion of other products that may also be suitable. 6/ Shuey, William C. A Wheat Sizing Technique for Predicting Flour Milling Yield. Cereal Science Today 5:71-72,75 (1960). The Uniform Regional Nursery spring wheat samples were milled in Brabender Quadrumat Senior mill heads. The stock from the Break head was sifted for 60 sec on a strand sifter using #35 and #80 Tyler sieves. The throughs of the #80 sieve were classified as break flour; the overs of the #35 sieve classified as bran; and the overs of the #80 sieve were passed through the reduction head of the mill. The reduction stock was sifted for 60 sec on a #80 Tyler sieve. The throughs were classified as reduction flour and the overs as shorts. The break and reduction flours were combined and classified as patent flour. The Field Plot Nursery samples were milled in a Buhler continuous experimental mill. The Buhler mill had been slightly modified for better comparison with commerical milling operations. Break scalping sieves were clothed with #54 stainless steel wire. Reduction scalping sieves were clothed with #58, #66 and #105 stainless steel wire for the first, second and third reductions, respectively. All flour sieves were clothed with #135 stainless steel wire. The six flour streams obtained from the Buhler-milled wheat were combined and classified as patent flour. The extraction of a good milling wheat using this flow is approximately 68% and is comparable to a commercial "long patent" extraction flour. At a 68% flour extraction, changes in flour ash are most sensitive to changes in percent extraction. Hardness Test - Wheat hardness scores were determined according to AACC Method 39-70A. The procedure involved grinding the wheat samples in a Udy grinder and obtaining reflectance data from a Technicon 450 near infrared analyzer. Wavelengths used were 1680 nm and 2230 nm. This procedure was developed by Mr. Karl Norris, USDA, Beltsville through a co-operative research project in which the Hard Red Spring and Durum Wheat Quality Laboratory also participated. Hard red spring wheats generally have NIR scores between 60 and 85. SKWCS Hardness Index - The single kernel wheat characterization system (SKWCS) instrument (developed by the GMRL, Manhattan, KS) was used to determine the hardness index and the percent distribution of kernels that were classified as soft, semi-soft, semi-hard, and hard. Protein Content - Wheat and flour proteins were determined from NIR reflectance data, the Kjeldahl procedure, or Leco Nitrogen determinations. Nitrogen values, as determined the Kjeldahl procedure or Leco, were multplied by 5.7 to calculate protein values. Values were reported on a 14%mb. Mineral or Ash Content - Wheat or flour ash was determined by measuring the residual weight of minerals remaining after incinerating the sample for approximately 16 hours at 575 deg. C. The results were reported as percentages of the sample weights. Values were reported on a 14%mb. Mixograph Analysis - Mixograph data was determined on each flour sample by using 30 g of flour (as is mb) and adding 20 cc of water. The sensitivity spring setting was set at 10. All mixograms were run with constant weight of flour and volume of water. Water absorptions were adjusted according to the peak heights of the mixograms. Correction factors were determined from a series of flours by varying the amount of absorption. Mixogram Patterns - Reference mixogram patterns shown on page17 illustrate different dough charactistics. A single number is assigned each pattern to characterize and simplify the classification of the curves. The larger numbers indicate stronger curve characteristics. Baking Procedure and Formula - Following is the baking formula used: 100% flour 3% Non-fat Dry Milk 2% salt 3% yeast 5% sugar 2% shortening (Crisco, melted) - 100 g loaves 1% shortening (Crisco, melted) - 25 g loaves Samples were mixed to optimum dough development in National Manufacturing mixers, the micro mixer for 25 g samples and the 100 g special mixer for 100 g samples. Bromate (10 ppm) for oxidation and Fungal Amylase (Doh-Tone)(15SKB units) for enzymatic supplement were added to each sample. All doughs were moulded in a Roll-Er-Up moulder. Samples undergo 3 hour fermentation, 1 hour proof and 20 minute bake time. Absorption - The amount of water, expressed as percent of flour, required for optimum dough consistency. Dough Characterictics (DC) - Emperical scores ranging from 0-6 were used to classify dough as sticky-weak (0), pliable (2), elastic (3), or bucky (6). The most favorable rating for optimum bread characteristics would be elastic. Crumb Color (CC) - A value was determined by comparing the internal crumb color of the sample bread with the crumb color of a baking standard. The standard flour was an equal blend of the variety Grandin grown at Casselton and Minot, ND, and Crookston, MN, and Brookings, SD. Emperical scores ranging from 0-6 were used to classify crumb color as yellow (0), grey (2), dull (3), creamy (5), or bright white (6). Crumb Grain (CG) - Emperical scores ranging from 0-6 were used to classify crumb grain as irregular, thick (0), open, thick (3), or fine (6). Crumb Texture (CT) - Emperical scores ranging from 0-6 were used to classify crumb texture as harsh (0), coarse (3), or silky (6). Loaf Volume - The volume (cc) of the baked loaf as determined by rapeseed displacement. DISCUSSION The following discussion presents the basic techniques and criteria used in the quality evaluation of the Hard Red Spring Wheat cultivars. For the Uniform Regional Nursery samples, each quality variable (except for bake absorption, mix time, and CG) per sample was averaged within each hard red spring wheat region (Northeast, Southeast, Midwest, and West). Values that are bolded/underlined indicate that averages are higher than the standard (Butte 86). For wheat and flour ash, bolded/underlined values are lower than the standard. All samples were compared with a milling and baking standard representative of the crop year. Agronomic and climatic conditions of the individual locations can affect the quality of the wheat such that the evaluation of all samples, including commercial cultivars, harvested from these locations may be classified as questionable to unsatisfactory. For example, an area may produce low protein wheat with large and plump kernels, good milling performance, and good kernel characteristics, but with low flour protein and unsatisfactory baking performance such as short mixing time, low loaf volume, and weak dough characteristics. The wheat from this area could not be considered a strong spring wheat and would not maintain the quality expected from the spring wheat producing area. An acceptable variety should have tolerance to a wide range of environmental conditions. Kernel Characteristics are important in determining the initial value of wheat. Poor kernel characterisitics could disqualify a new variety from further consideration. Because of the present wheat grading system, high test weight is desirable. Plump kernels are desirable because of their high ratio of endosperm to bran. Low 1000-kernel weight and small kernel size distribution affect milling performance due to their high ratio of bran to endosperm. Wheat ash is an important factor when comparing one cultivar against other standard cultivars. Wheat with a high mineral content may yield flour with a high ash content. Wheat protein quality and quantity must be considered as important characteristics when comparing cultivars grown at the same location. Wheats with low protein values are undesirable since protein affects baking performance. Milling Performance is a very important characteristic of spring wheats. Low extraction and high flour ash are major factors that are unacceptable under commercial milling operations. As a general rule, an increase of 0.01% in ash content is equivalent to an increase of approximately 2% in flour extraction. Milling characteristics: Wheat comprising soft kernels requires different milling techniques when compared with wheat of uniform hard kernels. With commercial mills flowed for hard vitreous spring wheats, the introduction of soft wheats into the mill will result in milling problems. Likewise, a sample which is extremely hard and vitreous will mill differently. Both types of wheat (soft and vitreous) require different roll pressures, clothing, sifter surface, and temper to be milled properly. The blending of normal bread wheats with soft wheats or extremely hard, vitreous wheats is undesirable since they are not compatible in the milling operation. Adjustments would either have to be made in the milling flow or in tempering procedures to compensate for differences in kernel hardness. Properties of soft wheat may or may not be compatible with other wheats. Therefore, maintaining pure varieties with uniform milling characteristics is important. The amount of protein recovered in flour from wheat is important. High protein wheats yielding low protein flours are not desirable. Such wheats would contain much of the protein distributed in the outer portion of the kernels resulting in excessive protein in the feed streams. Therefore, higher protein wheat would be necessary to yield a flour with protein content comparable to that of a wheat that yields optimum flour protein. Mixogram patterns are important in estimating the strength and mixing tolerance or potential mixing tolerance of a flour. From the standard mixogram patterns (page 17), patterns 6 - 8 indicate flours with optimum mixing tolerance and gluten strength. Mixogram patterns 9 - 11 indicate flour samples with long mixing times, and strong gluten characteristics, whereas, patterns 1 - 5 indicate flours with weak gluten characteristics and short mixing times. Both the pattern and length of the curve are important, and both must be considered in the evaluation. Abnormal curves, such as sway-back or long initial times to incorporate water, indicate undesirable characteristics. Baking evaluation takes into account the flour water absorption, mixing time, dough characteristics, loaf volume, crumb texture, and machinability. Flour samples with low water absorptions would be unsatisfactory. Samples with extremely short mixing times would relate to weak gluten characteristics and be considered undesirable. The crumb grain or appearance of the interior of the loaf indicates the sturctural integrity during baking. Crumb grain is likely related to gluten protein properties (quantity and quality). Bread loaf volume indicates potential strength of doughs in a different manner than mixing time or dough characteristics. Optimum loaf volume demonstrates the capacity, or lack thereof, for the dough to expand under pressure and to contain the entrapped gases during expansion. Weak doughs tend to collapse and yield breads with low loaf volumes, or yield breads with extremely large volumes containing large holes in the interior. Low protein flours produce extensible doughs that do not expand adequately during fermentation or baking and thus produce bread with low loaf volumes. Tough and very bucky doughs are bound too tightly and impede expansion of the gases resulting in breads with low loaf volume. Loaf volume is a characteristic probably related to gluten functionality in the dough. UNIFORM REGIONAL NURSERY SAMPLES - 1994 CROP A total of 586 samples were received from 19 stations in 8 states. Twenty-seven selections were experimental lines and the remainder were commercial cultivars. Commercial cultivars included Butte 86 (used as the standard for comparison), Chris, Era, Marquis, and Stoa. Each sample was evaluated for kernel characteristics, milling performance, and breadmaking properties.