TABLE OF CONTENTS
                    
 TABLE OF CONTENTS
 TITLE PAGE
 1994 COOPERATING AGENCIES AND STATIONS
 INTRODUCTION   
 SOURCE OF THE 1994 CROP SAMPLES
 UNIFORM REGIONAL NURSERY TRIALS
 ENTRIES IN THE UNIFORM REGIONAL HARD RED SPRING WHEAT PERFORMANCE NURSERY
 METHODS                         
 DISCUSSION                      
 UNIFORM REGIONAL NURSERY SAMPLES - 1994 CROP
 
 
 
 
 TITLE PAGE
                    UNITED STATES DEPARTMENT OF AGRICULTURE
                         AGRICULTURAL RESEARCH SERVICE
                              in cooperation with
                     STATE AGRICULTURAL EXPERIMENT STATION
                                        
             QUALITY EVALUATION OF HARD RED SPRING WHEAT CULTIVARS
                                        
                                  1994 CROP1/
                                        
                                       by
                                        
          G.A. Hareland, W.J. Newell, J.G. Wear,2/, and M. Skunberg3/
 
 ___________________________________________________________________________
 1/ This report represents cooperative investigations on the quality of Hard
 Red Spring Wheat cultivars from the 1994 crop.  Some of the results presented
 have not been sufficiently confirmed to justify varietal release.  Confirmed
 results will be published through established channels.  Cooperators
 submitting samples for analysis have been given analytical data on their
 samples prior to release of this report.  This report is primarily a tool for
 use by cooperators and their official staff and to those individuals having
 direct and special interest in the development of agricultural research
 programs.
 
 This report was compiled by the Agricultural Research Service, U. S.
 Department of Agriculture.  Special acknowledgment is made to the North Dakota
 State University for use of their facilities and the services provided in
 support of these studies.  The report is not intended for publication and
 should not be referenced in either literature citations or quoted in publicity
 and advertising.  Use of the data may be granted for certain purposes upon
 written request to the agency or agencies involved.
 
 2/ Research Food Technologist and Physical Science Technicians, USDA/ARS Hard
 Red Spring & Durum Wheat Quality Laboratory, NDSU, Fargo, ND.
 
 3/ Food Technologist, Dept. of Cereal Science & Food Technology, NDSU, Fargo,
 ND.
 
 
 
 
 
 1994 COOPERATING AGENCIES AND STATIONS
 
 The cooperative agencies and stations conducting the varietal plot and nursery
 experiments from which the 1994 spring wheat samples were received are listed
 below:
 
                     Idaho Agricultural Experiment Station
                               Aberdeen, Tetonia
                                        
                   Minnesota Agricultural Experiment Station
                                Morris, St. Paul
                                        
                    Montana Agricultural Experiment Station
                             Bozeman, Havre, Sidney
                                        
                  North Dakota Agricultural Experiment Station
                     Minot, Langdon, Dickinson, Casselton,
                         Carrington, Prosper, Williston
                                        
                  South Dakota Agricultural Experiment Station
                            Brookings, Groton, Selby
                                        
                   Washington Agricultural Experiment Station
                                    Pullman
                                        
                   Wisconsin Agricultural Experiment Station
                                    Madison
                                        
                    Wyoming Agricultural Experiment Station
                                     Powell
 
 
 A complete list of all cooperating agencies, stations, and personnel for the
 year will be found in the report by R. H. Busch, et al., Wheat Varieties Grown
 in Cooperative Plot and Nursery Experiments in the Spring Wheat Region in
 1994.4/
 
 _____________________________________________________________________________
 4/ Busch, R. H.  Wheat Varieties Grown in Cooperative Plot and Nursery
 Experiments in the Spring Wheat Region in 1994.  Agricultural Research
 Service, U. S. Department of Agriculture and State Agricultural Experiment
 Station, St. Paul, MN.
 
 
 
 
 
 
 INTRODUCTION
 
 Samples of standard cultivars and new selections of hard red spring wheat
 grown in cooperative experiments in spring wheat regions of the United States
 are milled each year by the USDA/ARS, Wheat Quality Laboratory.  Wheat and
 their corresponding flours are evaluated for physical and chemical properties,
 and the flours are baked to determine bread characteristics.  The purpose of
 this report is to make available to the cooperators and other interested
 parties, quality data on the standard varieties and new selections of hard red
 spring wheat from the 1994 crop.
 
 The evaluation of a wheat sample involves the analysis of kernel
 characteristics, milling performance, and baking performance.  A brief
 description of testing methods employed is shown on pages 9-12.  No specific
 comments are made regarding mixogram patterns derived from samples.  However,
 reference mixograms, shown on page 17, illustrate ranges from which sample
 mixograms may be compared.
 
 
 
 
 
 
 SOURCE OF THE 1994 CROP SAMPLES
 
 Tests were performed on 1211 samples which were received from 24 stations in
 10 states.  However, data on 625 samples is excluded from this report, because
 the information was of interest only to plant breeders at specific experiment
 stations.
 
 Data presented in this report represents the evaluation of spring wheats
 received from Field Plot Nurseries and Uniform Regional Nurseries.  The
 following stations were cooperators:
 
                 Idaho:          Aberdeen, Tetonia
                 Minnesota:      Morris and St. Paul
                 Montana:        Bozeman, Havre, Sidney 
                 North Dakota:   Minot, Langdon, Dickinson, Casselton,
                                 Williston, Carrington, Prosper
                 South Dakota:   Brookings, Groton, Selby
                 Washington:     Pullman
                 Wisconsin:      Madison
                 Wyoming:        Powell
 
         
 
 
 
 
 UNIFORM REGIONAL NURSERY TRIALS
 
 The geographical areas from which the samples were received are shown on page
 7.  Spring wheat cultivars and experimental lines included in the Uniform
 Regional Nursery trials are listed on page 8.  The Northeast area included 4
 stations; the Southeast area included 6 stations; the Midwest area included 4
 stations; and the West area included 5 stations.
 
 
 
                                   
 
 ENTRIES IN THE UNIFORM REGIONAL HARD RED SPRING WHEAT PERFORMANCE NURSERY
 
 The 32 entries in the 1994 URHRSWPN are listed below:
 
 Entry  Cross or  CI No. or                            Year     
 No.    Variety    Selection No.                      Entered     Source 
 
 1.    Marquis    3561                                  1929      Canada
 2.    Chris      13751                                 1969      USDA-MN
 3.    Era        13986                                 1972      USDA-MN
 4.    Stoa                                             1987      ND
 5.    Butte 86                                         1987      ND
 6.    SD0007     MN7663/SBY354A                        1994      SD
 7.    SD0010     YW352/SBZ004A                         1993      SD
 8.    SD0014     YW352/SBZ004A                         1994      SD
 9.    SD3151     SD3036//SD8052/SD2971                 1994      SD
 10.   SD3156     Butte 86/SD8061                       1994      SD
 11.   SBE0437    MN7663/SBY354A                        1993      USDA-MN
 12.   MN90138    MN86400/MN86033                       1994      USDA-MN
 13.   MN91227    MN87003/MN86497                       1994      USDA-MN
 14.   MN91309    SBI0037                               1994      USDA-MN
 15.   MN91324    SBI0072                               1994      USDA-MN
 16.   MN92006    Marshall/MN88076                      1994      USDA-MN
 17.   SBE0050    W8814/Norak                           1994      USDA-MN
 18.   ND673      Grandin/Stoa 'S'                      1992      ND
 19.   ND674      Grandin*2/ND6453                      1993      ND
 20.   ND677*     ND622*2/Cutless                       1993      ND
 21.   ND678      Stoa'S'/3/IAS20*4/H567.71             1993      ND
                  //Amidon
 22.   ND686      Grandin'S'/3/Colonia                  1994      ND
                  S/Amidon//Grand
 23.   N90-0671   Bergen/N86-0111                       1993      AGPRO
 24.   N90-0666   Bergen/N86-0111                       1993      AGPRO
 25.   N90-0700   Prospect/Amidon                       1993      AGPRO
 26.   N89-0562   CNO'S'/7C/3/B.RE//BA                  1994      AGPRO
                  PET/ANB 
 27.   N91-0059   HS85-510/Success                      1994      AGPRO
 28.   N90-0392   HS85-0476/HS84-0700                   1994      AGPRO
 29.   N91-3057   B85-1525/Krona                        1994      AGPRO
 30.   BW688      BW608/Norseman                        1994      AGCAN
 31.   BW173      COL*2//Saric/NEP/3/COL*5//Saric/NEP   1994      AGCAN
 32.   BW174      COL*2//Saric/NEP/3/COL*5//Saric/NEP   1994      AGCAN
      
     * Solid stem lines for sawfly resistance, 
                            
 
 
 
 METHODS
 
 Following are the variables and testing methods used in the evaluation
 process:
 
 Test Weight Per Bushel - The weight per Winchester bushel of cleaned, dry
 wheat subsequent to passing the sample through a Carter-Day dockage tester.
 
 1000-Kernel Weight - The weight of 1000 kernels was determined by counting,
 using a Seedburo seed counter, the number of kernels in 10 g samples of
 cleaned, hand- picked wheat.5/
 
 Kernel Size - The percentages of the size of kernels (large, medium and small)
 were determined using a wheat sizer as described by Shuey6/.
 
 The sieves of the sizer were clothed as follows:
 
                 Top Sieve    - Tyler #7 with 2.92 mm opening
                 Middle Sieve - Tyler #9 with 2.24 mm opening
                 Bottom Sieve - Tyler #12 with 1.65 mm opening
 
 Milling - The samples were cleaned by passing the wheat through a Carter-Day
 dockage tester and through a modified Forster scourer (Model 6).  The clean,
 dry samples were pretempered to 12.5% moisture for at least 72 hours, then
 tempered to 15.5% moisture and allowed to stand overnight prior to milling.
 
 ____________________________________________________________________________
 5/ Mention of a trademark name or a proprietary product does not constitute a
 guarantee or warranty of the product by the U. S. Department of Agriculture,
 and does not imply its approval to the exclusion of other products that may
 also be suitable.
 
 6/ Shuey, William C.  A Wheat Sizing Technique for Predicting Flour Milling
 Yield.  Cereal Science Today 5:71-72,75 (1960).
 
 
 
 
 
 
 
 
 
 
 The Uniform Regional Nursery spring wheat samples were milled in Brabender
 Quadrumat Senior mill heads.  The stock from the Break head was sifted for 60
 sec on a strand sifter using #35 and #80 Tyler sieves.  The throughs of the
 #80 sieve were classified as break flour; the overs of the #35 sieve
 classified as bran; and the overs of the #80 sieve were passed through the
 reduction head of the mill.  The reduction stock was sifted for 60 sec on a
 #80 Tyler sieve.  The throughs were classified as reduction flour and the
 overs as shorts.  The break and reduction flours were combined and classified
 as patent flour.
 
 The Field Plot Nursery samples were milled in a Buhler continuous experimental
 mill.  The Buhler mill had been slightly modified for better comparison with
 commerical milling operations.  Break scalping sieves were clothed with #54
 stainless steel wire.  Reduction scalping sieves were clothed with #58, #66
 and #105 stainless steel wire for the first, second and third reductions,
 respectively.  All flour sieves were clothed with #135 stainless steel wire.
 
 The six flour streams obtained from the Buhler-milled wheat were combined and
 classified as patent flour.  The extraction of a good milling wheat using this
 flow is approximately 68% and is comparable to a commercial "long patent"
 extraction flour.  At a 68% flour extraction, changes in flour ash are most
 sensitive to changes in percent extraction.
 
 Hardness Test - Wheat hardness scores were determined according to AACC Method
 39-70A.  The procedure involved grinding the wheat samples in a Udy grinder
 and obtaining reflectance data from a Technicon 450 near infrared analyzer.
 Wavelengths used were 1680 nm and 2230 nm.  This procedure was developed by
 Mr. Karl Norris, USDA, Beltsville through a co-operative research project in
 which the Hard Red Spring and Durum Wheat Quality Laboratory also
 participated.  Hard red spring wheats generally have NIR scores between 60 and
 85.
 
 SKWCS Hardness Index - The single kernel wheat characterization system (SKWCS)
 instrument (developed by the GMRL, Manhattan, KS) was used to determine the
 hardness index and the percent distribution of kernels that were classified as
 soft, semi-soft, semi-hard, and hard.
 
 Protein Content - Wheat and flour proteins were determined from NIR
 reflectance data, the Kjeldahl procedure, or Leco Nitrogen determinations.
 Nitrogen values, as determined the Kjeldahl procedure or Leco, were multplied
 by 5.7 to calculate protein values.  Values were reported on a 14%mb.
 
 Mineral or Ash Content - Wheat or flour ash was determined by measuring the
 residual weight of minerals remaining after incinerating the sample for
 approximately 16 hours at 575 deg. C.  The results were reported as
 percentages of the sample weights.  Values were reported on a 14%mb.
 
 Mixograph Analysis - Mixograph data was determined on each flour sample by
 using 30 g of flour (as is mb) and adding 20 cc of water.  The sensitivity
 spring setting was set at 10.  All mixograms were run with constant weight of
 flour and volume of water.  Water absorptions were adjusted according to the
 peak heights of the mixograms.  Correction factors were determined from a
 series of flours by varying the amount of absorption.
 
 Mixogram Patterns - Reference mixogram patterns shown on page17 illustrate
 different dough charactistics.  A single number is assigned each pattern to
 characterize and simplify the classification of the curves.  The larger
 numbers indicate stronger curve characteristics.
 
 Baking Procedure and Formula - Following is the baking formula used:
 
         100% flour              3% Non-fat Dry Milk
          2% salt                3% yeast
          5% sugar               2% shortening (Crisco, melted) - 100 g loaves
                                 1% shortening (Crisco, melted) - 25 g loaves
 
 Samples were mixed to optimum dough development in National Manufacturing
 mixers, the micro mixer for 25 g samples and the 100 g special mixer for 100 g
 samples.  Bromate (10 ppm) for oxidation and Fungal Amylase (Doh-Tone)(15SKB
 units) for enzymatic supplement were added to each sample.  All doughs were
 moulded in a Roll-Er-Up moulder.  Samples undergo 3 hour fermentation, 1 hour
 proof and 20 minute bake time.
 
 Absorption - The amount of water, expressed as percent of flour, required for
 optimum dough consistency.
 
 Dough Characterictics (DC) - Emperical scores ranging from 0-6 were used to
 classify dough as sticky-weak (0), pliable (2), elastic (3), or bucky (6).
 The most favorable rating for optimum bread characteristics would be elastic.
 
 Crumb Color (CC) - A value was determined by comparing the internal crumb
 color of the sample bread with the crumb color of a baking standard.  The
 standard flour was an equal blend of the variety Grandin grown at Casselton
 and Minot, ND, and Crookston, MN, and Brookings, SD.  Emperical scores ranging
 from 0-6 were used to classify crumb color as yellow (0), grey (2), dull (3),
 creamy (5), or bright white (6).
 
 Crumb Grain (CG) - Emperical scores ranging from 0-6 were used to classify
 crumb grain as irregular, thick (0), open, thick (3), or fine (6).
 
 Crumb Texture (CT) - Emperical scores ranging from 0-6 were used to classify
 crumb texture as harsh (0), coarse (3), or silky (6).
 
 Loaf Volume - The volume (cc) of the baked loaf as determined by rapeseed
 displacement.
 
 
 
                      
 DISCUSSION
 
 The following discussion presents the basic techniques and criteria used in
 the quality evaluation of the Hard Red Spring Wheat cultivars.
 
 For the Uniform Regional Nursery samples, each quality variable (except for
 bake absorption, mix time, and CG) per sample was averaged within each hard
 red spring wheat region (Northeast, Southeast, Midwest, and West).  Values
 that are bolded/underlined indicate that averages are higher than the standard
 (Butte 86).  For wheat and flour ash, bolded/underlined values are lower than
 the standard.
 
 All samples were compared with a milling and baking standard representative of
 the crop year.  Agronomic and climatic conditions of the individual locations
 can affect the quality of the wheat such that the evaluation of all samples,
 including commercial cultivars, harvested from these locations may be
 classified as questionable to unsatisfactory.  For example, an area may
 produce low protein wheat with large and plump kernels, good milling
 performance, and good kernel characteristics, but with low flour protein and
 unsatisfactory baking performance such as short mixing time, low loaf volume,
 and weak dough characteristics.  The wheat from this area could not be
 considered a strong spring wheat and would not maintain the quality expected
 from the spring wheat producing area.  An acceptable variety should have
 tolerance to a wide range of environmental conditions.
 
 Kernel Characteristics are important in determining the initial value of
 wheat.  Poor kernel characterisitics could disqualify a new variety from
 further consideration.  Because of the present wheat grading system, high test
 weight is desirable.  Plump kernels are desirable because of their high ratio
 of endosperm to bran.  Low 1000-kernel weight and small kernel size
 distribution affect milling performance due to their high ratio of bran to
 endosperm.  Wheat ash is an important factor when comparing one cultivar
 against other standard cultivars.  Wheat with a high mineral content may yield
 flour with a high ash content.  Wheat protein quality and quantity must be
 considered as important characteristics when comparing cultivars grown at the
 same location.  Wheats with low protein values are undesirable since protein
 affects baking performance.
 
 Milling Performance is a very important characteristic of spring wheats.  Low
 extraction and high flour ash are major factors that are unacceptable under
 commercial milling operations.  As a general rule, an increase of 0.01% in ash
 content is equivalent to an increase of approximately 2% in flour extraction.
 
 Milling characteristics: Wheat comprising soft kernels requires different
 milling techniques when compared with wheat of uniform hard kernels.  With
 commercial mills flowed for hard vitreous spring wheats, the introduction of
 soft wheats into the mill will result in milling problems.  Likewise, a sample
 which is extremely hard and vitreous will mill differently.  Both types of
 wheat (soft and vitreous) require different roll pressures, clothing, sifter
 surface, and temper to be milled properly.  The blending of normal bread
 wheats with soft wheats or extremely hard, vitreous wheats is undesirable
 since they are not compatible in the milling operation.  Adjustments would
 either have to be made in the milling flow or in tempering procedures to
 compensate for differences in kernel hardness.  Properties of soft wheat may
 or may not be compatible with other wheats.  Therefore, maintaining pure
 varieties with uniform milling characteristics is important.
 
 The amount of protein recovered in flour from wheat is important.  High
 protein wheats yielding low protein flours are not desirable.  Such wheats
 would contain much of the protein distributed in the outer portion of the
 kernels resulting in excessive protein in the feed streams.  Therefore, higher
 protein wheat would be necessary to yield a flour with protein content
 comparable to that of a wheat that yields optimum flour protein.
 
 Mixogram patterns are important in estimating the strength and mixing
 tolerance or potential mixing tolerance of a flour.  From the standard
 mixogram patterns (page 17), patterns 6 - 8 indicate flours with optimum
 mixing tolerance and gluten strength.  Mixogram patterns 9 - 11 indicate flour
 samples with long mixing times, and strong gluten characteristics, whereas,
 patterns 1 - 5 indicate flours with weak gluten characteristics and short
 mixing times.  Both the pattern and length of the curve are important, and
 both must be considered in the evaluation.  Abnormal curves, such as sway-back
 or long initial times to incorporate water, indicate undesirable
 characteristics.
 
 Baking evaluation takes into account the flour water absorption, mixing time,
 dough characteristics, loaf volume, crumb texture, and machinability.  Flour
 samples with low water absorptions would be unsatisfactory.  Samples with
 extremely short mixing times would relate to weak gluten characteristics and
 be considered undesirable.
 
 The crumb grain or appearance of the interior of the loaf indicates the
 sturctural integrity during baking.  Crumb grain is likely related to gluten
 protein properties (quantity and quality).
 
 Bread loaf volume indicates potential strength of doughs in a different manner
 than mixing time or dough characteristics.  Optimum loaf volume demonstrates
 the capacity, or lack thereof, for the dough to expand under pressure and to
 contain the entrapped gases during expansion.  Weak doughs tend to collapse
 and yield breads with low loaf volumes, or yield breads with extremely large
 volumes containing large holes in the interior.  Low protein flours produce
 extensible doughs that do not expand adequately during fermentation or baking
 and thus produce bread with low loaf volumes.  Tough and very bucky doughs are
 bound too tightly and impede expansion of the gases resulting in breads with
 low loaf volume.  Loaf volume is a characteristic probably related to gluten
 functionality in the dough.
 
 UNIFORM REGIONAL NURSERY SAMPLES - 1994 CROP
 
 A total of 586 samples were received from 19 stations in 8 states.
 Twenty-seven selections were experimental lines and the remainder were
 commercial cultivars.  Commercial cultivars included Butte 86 (used as the
 standard for comparison), Chris, Era, Marquis, and Stoa.  Each sample was
 evaluated for kernel characteristics, milling performance, and breadmaking
 properties.