IV. 18. The genetic male sterile barley collection.
E.A. Hockett. Western Region, AR, SEA, USDA, and Plant and Soil Science Department, Montana State University, Bozeman, Montana 59717, U.S.A.
Five new numbered loci have been assigned to the genetic male sterile series as shown in Table 1. These results of crossing a homozygous male sterile plant with a heterozygous male sterile plant show that msg29a, msg30c, msg31d, msg32w, and msg33x are not allelic with any of the previously numbered loci or each other. All five of these mutants segregate 3:1 for male sterility (2 and 3); msg29a, msg31d, and msg32w set no seed on selfing and msg33x sets 0-29.6% selfs depending on the environment under which it is grown (2 and 6); and msg30c has some selfing (2) and female sterility (6). The mutant msg31d is linked with a dwarfing gene (Hockett, unpublished data).
Table 2 gives data for three mutants found to be allelic with previously numbered or designated loci. Although msg23y did not fit the expected 1:1 ratio of fertile:sterile F1 plants with msg23b, it had a satisfactory fit with msg23bg. More selfing was found at Bozeman with msg23y, 1.9 to 13.8% (6) than with msg23b, 0% (2), or with msg23bg, 0% (3). However, msg23b did set some selfed seed at Tucson (2). The segregation and selfing behavior of msg,,dl is found in the 1975 report (4). Ahokas (1) also found that ms,,dm and msg,,dn were allelic. Although the presumed selfed seeds of ms,,dm and ms,,dn mentioned in the 1977 report (5) were planted in 1977, no plants were obtained and thus the question of self or outcrosses for these mutants is unanswered.
Table 2. Ratios of fertile to male sterile F1 plants from crosses of genes determined to be allelic.
The segregation ratios, selfing behavior and origin of 13 new mutants is shown in Table 3. Pooled data for 1977 and 1978 indicates that all the mutants represent single genes for male sterility. Six of the mutants show no selfing in either 1977 or 1978, four self in both years, two selfed only in 1977, and one mutant has data in only one year.
References:
1. Ahokas, H. l978. Male sterile mutants of barley VI. Tests of restoration by the cytoplasm of Hordeum bulbosum, and reports of new mutants. Barley Genet. Newsl. 8:12-15.
2. Hockett, E. A. and R. F. Eslick. 1971. Genetic male sterile genes useful in hybrid barley production. Barley Cenetics II: 308-315. Wash. State Univ. Press, Pullman, Wash.
3. Hockett, R. A. 1972. Coordinator's report on the genetic male sterile barley collection. Barley Genet. Newsl. 2:139-144.
4. Hockett, E. A. 1975. The genetic male sterile barley collection. Barley Genet. Newsl. 5:84-86.
5. Hockett, E. A. 1976. The genetic male sterile barley collection. Barley Genet. Newsl. 7:97-100.
6. Hockett, E. A. and H. Ahokas. 1979. Male and female fertllity levels of genetic male sterile barley grown at two different latitudes. Hereditas. (In press).
