II. 41 Protein and lysine maternal inheritance effects in high protein and high lysine barley mutant Hiproly. (1)
S.E. Ullrich and R.F. Eslick. Department of Plant and Soil Science, Montana State University, Bozeman, Montana 59717 "R".
(1)Supported in part by USA AID/ta-C-1094.
To determine if there are maternal effects in the inheritance of protein and/or lysine in Hiproly, reciprocal crosses were made between Hiproly and the "normal" barley variety Betzes. Twelve F1 seed from each cross were screened for lysine content by a microbioiogical agar plate technique (Sands, Dull and Eslick, manuscript in preparation). This screen indicated that the F1 seed from the Hiproly x Betzes cross were higher in lysine than the Betzes x Hiproly F1 seed.
The F2 seed from four F1 plants from each cross were analyzed for protein by the macro Kjeldahl technique (N x 6.25) and for lysine by a modified microbiological assay (Waters, 1976). Both percent protein and percent lysine were higher in the F2 seed from the Hiproly x Betzes cross than in the Betzes x Hiproly cross.
These results provide evidence for maternal effects in the inheritance of protein and lysine in Hirpoly. Endosperm dosage effects alone cannot account for the significant differences obtained in both the F1 and F2 seed generations. Indirect supportive evidence for these conclusions is presented by Peruanskii et al. (1976).
According to S.O.S. Thomke (personal communication), Swedish investigators have found that an earlier than normal constriction of the vascular bundles entering the kernels occurs in Hiproly. This could explain the higher lysine content of F1 seed harvested from Hiproly plants as contrasted to those F1 seed harvested from Betzes plants. The difference in F2 seed from reciprocal crosses cannot be explained on this basis. Cytoplasmic inheritance could explain the results of both types of progenies. The data suggests additional information is needed for Hiproly and other high lysine mutants.
References:
Peruanskii, YU, V., S.S. Sadykov, O.N. Peruanskaya and V.S. Lebedeva. 1976. Features of the amino acid composition of the grain in hybrids of the barley Hiproly. Selektsiya I Sfmenovodstvo 6:28-29.
Waters, R.F. 1976. A modified microbiological assay for lysine determination in Hordeum spp. Ph.D. dissertation, Montana State Univ., Univ. Microfilms, Ann Arbor, Michigan (Diss. Abstr. 37:2665).
