II.24 Location of vacuole initiation in microspores in barley.
M. Okamoto and G. Röbbelen. Institut für Pflanzenzüchtung, University, Göttingen, Von Siebolstr. 8, FR Germany.
Kihara and Hirayoshi (1942) reported that large vacuoles developed at the central part of microspores in three varieties of Oryza sativa L.; but they did not point out the place where vacuoles would be initiated. Their results were based on observations of materials fixed in Navashin's fluid.
The writers studied vacuole development with special attention to the place where vacuoles would be initiated in microspores at uninucleate stage. Fifty cells from one anther each of 10 plants of the spring barley cultivar "Asse" grown at around 15°C at night and about 20°C to 25°C in the daytime were observed as to the location of vacuole development. Anthers were cut into two and very gently tapped over cover glass to let microspores float out in aceto carmine.
In all of the microspores initial vacuole development was observed at the periphery of microspores without any exception. This observation can, however, only be made with fresh material. After more than 10 min in carmine drastic rearrangements within the cytoplasm of microspores can be seen, which also may dislocate the vacuoles from their original point of origin.
References:
H. Kihara and I. Hirayoshi: Entwicklung des Pollenkorns bei Oroza sativa L. (Japan.). Agr. and Hort. (Tokyo) 17 (6): 684-690 (1942).