II.24 Linkage of male sterile genes with seedling lethal genes.
M. M. Rahman and R. F. Eslick. Kentucky State University, Frankfort, Kentucky 40601 and Dept. of Plant and Soil Science, Montana State University, Bozeman, Montana 59715, respectively.
Some of the proposed systems for the production of the female stock for hybrid barley include the use of seedling lethal genes associated with genetic male sterile genes, Eslick (1970) and Ramage, et al. (1972). Wiebe, et al. (1974) have suggested the use of seedling lethals to serve as "chopper stoppers" in 8-paired barley lines.
We have determined recombination percentages for a number of male sterile genes and seedling lethal combinations, Table 1. All lethal genes and male sterile stocks are from the BGS (barley genetic stock) series as given in Barley Genetics Newsletters 1 through 4. F3 crossover genotypes were verified by continuing into the F4. Recombination percentages were determined by the maximum likelihood method.
Table. 2. Linkage intensities of male sterile genes and previously assigned seedling lethal genes.
Of the previously mapped seedling lethals, ac2, a2, y, xs, and ac were probably in close enough proximity to a known male sterile gene to be used in some hybrid systems.
The seedling lethals yc, at, cm, cm2, and xn may be far enough from the centromeres to be effective "chopper stoppers" in 8-paired barley lines.
References:
Eslick, R. F. 1970. Balanced male sterile and dominant pre-flowering selective genes for use in hybrid seed production. Barley Genetics II. Proc. Second Int. Barley Genet. Symp. pp. 292-296.
Ramage, R. T., G. A. Wiebe, R. F. Eslick, and R. K. Thompson. 1972. Use of mutagenic agents in hybrid barley breeding. 1971 Barley Newsletter 15: 73-78.
Wiebe, G. A., R. T. Ramage, and R. F. Eslick, 1974. Eight-paired barley lines, BGN 4: 93-95.
