II.5. Allele tests of gs and cer mutants.
R. F. Eslick, Department of Plant and Soil Science, Montana State University, Bozeman, Montana 59715, USA.
Since reporting on allelism among gs mutants BGN 1:31-34 we have been allele testing gs mutants against cer mutants and the results are presented in Table 1. To avoid duplication we do not intend to pursue this further but our results might be helpful to those who do.
gs1 (chromosome 4)
Our stock of gs1 was determined to be allelic to cer-q. Lundqvist and von Wettstein, Hereditas 48:342-362, also reported allelism for gs1 and cer-q.
gs2 (chromosome 3)
We have allele tested most of the Swedish mutants against gs2 and have not determined any allelism to date. Phenotypically there are three similar untested mutants cer-x, cer-z and cer-zl. Sogaard, BGN 1:41-47, assigned cer-x to chromosome 7, but failed to verify it's presence in crosses with several marker genes, BGN 3:57-61. The latter two mutants have not been assigned to chromosomes. Other somewhat similar mutants not allele tested are cer-m, cer-zi (on chromosome 5), cer-zu, cer-zw, and cer-zx.
gs3 (chromosome 1)
Our results confirm those of Tsuchiya, BGN 3: 67-68., i.e. gs3 is allelic to cer-a.
gs4 (chromosome 6)
Walker, et. al., Can. Jour. Gen. and Cyt. 5:200-219, on the basis F2 repulsion data found gl4 to have a recombination value of 30.4 + 6.7 with o and gs4 a recombination value of 29.4 + 2.6 with gl4, and assigned both genes to chromosome 6. Subsequently Tsuchiya, BGN 3:66-67, found gl4 to be allelic to gl3 on chromosome 4. Our unpublished results verify that gs4 should still be assigned to chromosome 6. This suggests that gs4 should be allele tested with the unassigned Swedish mutants cer-z and cer-zl.
Table 1. Alleles tests of gs and cer
mutants.
Continued to P. 20.
gs5 (chromosome 2)
The gs5 mutant was determined to be allelic to cer-s to which it is phenotypically similar.
gs6 (chromosome 2)
Our crosses would indicate that gs6 and cer-c are allelic. However, this would provide some disagreement in the literature. Takahashi and Hayashi, Ber. Ohara Inst. Landw. Forsch. 10:29-52, assigned subjacent hood, sk, and glossy sheath 6, gs6, to chromosome 2 from crosses with trisomics, and Tsuchiya, BGN 2:93-98, assigned these two genes to the short arm of chromosome 2 from telotrisomic crosses. Takahashi et. al. BGN 2:74-75 reported no linkage of sk or gs6 with e, v, li, and or on chromosome 2 but that sk and gs6 were linked. Recently Sogaard, BGN 3:57-61 has assigned cer-c to chromosome 4 by means of trisomics. The gs6 X cer-c cross should be repeated, preferable with stocks other than ours.
