REPORTS OF THE COORDINATORS
Overall coordinator’s report
Udda Lundqvist
SvalöfWeibull AB
SE-268 81 Svalöv, Sweden
e-mail: udda@ngb.se
Since the latest overall coordinator’s report in Barley Genetics Newsletter Volume 33, not many important changes of the coordinators have been reported. I do hope that most of you are willing to continue with this work and provide us with new important information and literature search in the future. Unhappily some of the coordinators have definitely retired from their positions or they do not find the time to prepare reports because of other commitments, or they have stopped working on barley research. The coordinator for Chromosome 3H, Roger Ellis, has retired from his position at the Scottish Crop Research Institute, United Kingdom, and we need to find a replacement. Diter von Wettstein, the coordinator for Chloroplast genes has desired to pass over this duty to Mats Hansson at the Department of Biochemistry, Lund University, Lund, Sweden. He promised to make this coordination as he intensively works on these problems. The coordinators for the Inversions, Bengt-Olle Bengtsson and Torbjörn Säll, both from the Institute of Genetics at the University of Lund, Sweden, asked to resign as they are not working with barley genetic research any more. I want to take the opportunity to thank all of them for their good corporation and their reliability of sending informative reports during all the years.
At the end of June, many of us met at the 9th International Barley Genetics Symposium in Brno, Czech Republic, and during a ‘Barley Genetic Linkage Workshop’ where it got intensively discussed if the current system and trait coordination should continue in this manner. I became decided to do so but with a view towards whole genome coordination in the future. The report of this workshop will be published in this or next BGN Volume.
Problems of minor modifications of Rule 6 and 7 of Gene Nomenclature were discussed and accepted at the Barley Genetic Linkage Workshop of the 9th International Barley Genetics Symposium in Brno, Czech republic, on June 19, 2004. Rules for Nomenclature and Gene Symbolization in Barley with the additional amendments will be published in this volume. Revised lists of BGS descriptions by BGS number (Table 1) and by locus symbols in alphabetic order (Table 2) are also published in this volume.
Chromoosome 1H (5): Gunter Backes, Plant Research Department, Risø National Laboratory, PRD-330, P.O.49, DK-4000 Roskilde, Denmark. FAX: +45 46 77 4282; e-mail: <gunter.backes@risoe.dk>
Chromosome 2H (2): Jerry. D. Franckowiak, Department of Plant Sciences, North Dakota State University, P.O.Box 5051, Fargo, ND 58105-5051, USA. FAX: +1 701 231 8474; e-mail: <j_franckowiak@ndsu.nodak.edu>
Chromosome 3H (3): Roger P. Ellis, Cell and Molecular Genetics Department, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, United Kingdom. FAX: +44 1382 562426. E-mail: <R.Ellis@scri.sari.ac.uk>
Chromosome 4H (4): Brian P. Forster, Cell and Molecular Genetics Department, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, United Kingdom. FAX: +44 1382 562426. e-mail: <bforst@scri.sari.ac.uk>
Chromosome 5H (7): George Fedak, Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, ECORC, Ottawa, ON, Canada K1A 0C6, FAX: +1 613 759 6559; e-mail: <fedakga@agr.gc.ca>
Chromosome 6H (6): Duane Falk, Department of Crop Science, University of Guelph, Guelph, ON, Canada, N1G 2W1. FAX: +1 519 763 8933; e-mail: <dfalk@uoguelph.ca>
Chromosome 7H (1): Lynn Dahleen, USDA-ARS, State University Station, P.O. Box 5677, Fargo, ND 58105, USA. FAX: + 1 701 239 1369; e-mail: <DAHLEENL@fargo.ars.usda.gov>
Integration of molecular and morphological marker maps: Andy Kleinhofs, Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164-6420, USA. FAX: +1 509 335 8674; e-mail: <andyk@wsu.edu>
Barley Genetics Stock Center: An Hang, USDA-ARS, National Small Grains Germplasm Research Facility, 1691 S. 2700 W., Aberdeen, ID 83210, USA. FAX: +1 208 397 4165; e-mail: <anhang@uidaho.edu>
Trisomic and aneuploid stocks: An Hang, USDA-ARS, National Small Grains Germplasm Research Facility, 1691 S. 2700 W., Aberdeen, ID 83210, USA. FAX: +1 208 397 4165; e-mail: <anhang@uidaho.edu>
Translocations and balanced tertiary trisomics: Andreas Houben, Institute of Plant Genetics and Crop Plant Research, Corrensstrasse 3, DE-06466 Gatersleben, Germany. FAX: +49 39482 5137; e-mail: <houben@ipk-gatersleben.de>
Desynaptic genes: Andreas Houben, Institute of Plant Genetics and Crop Plant Research, Corrensstrasse 3, DE-06466 Gatersleben, Germany. FAX: +49 39482 5137; e-mail: <houben@ipk-gatersleben.de>
Autotetraploids: Wolfgang Friedt, Institute of Crop Science and Plant Breeding, Justus-Liebig-University, Heinrich-Buff-Ring 26-32, DE-35392 Giessen, Germany. FAX: +49 641 9937429; e-mail: <wolfgang.friedt@agrar.uni-giessen.de>
Eceriferum genes: Udda Lundqvist, Svalöf Weibull AB, SE-268 81 Svalöv, Sweden. FAX:.+46 418 667109; e-mail: <udda@ngb.se>
Chloroplast genes: Diter von Wettstein, Department of Crop and Soil Sciences, Genetics and Cell Biology, Washington State University, Pullman, WA 99164-6420, USA. FAX: +1 509 335 8674; e-mail: <diter@wsu.edu>
Genetic male sterile genes: Mario C. Therrien, Agriculture and Agri-Food Canada, P.O. Box 1000A, R.R. #3, Brandon, MB, Canada R7A 5Y3, FAX: +1 204 728 3858; e-mail: <MTherrien@agr.gc.ca>
Inversions: Bengt-Olle Bengtsson, Institute of Genetics, University of Lund, Sölvegatan 29, SE-223 62 Lund, Sweden. FAX: +46 46 147874;
e-mail: <bengt_olle.bengtsson@cob.lu.se> and
Torbjörn Säll, Institute of Genetics, University of Lund, Sölvegatan 29, SE-223 62 Lund Sweden. FAX: +46 46 147874, e-mail:<torbjorn.sall@cob.lu.se>
Ear morphology genes: Udda Lundqvist, Svalöf Weibull AB, SE-268 81 Svalöv, Sweden. FAX: +46 418 667109; e-mail: <udda@ngb.se>
Semi-dwarf genes: Jerry D. Franckowiak, Department of Plant Sciences, North Dakota State University, P.O. Box 5051, Fargo, ND 58105-5051, USA. FAX: +1 702 231 8474; e-mail: <j_franckowiak@ndsu.nodak.edu>
Early maturity genes: Udda Lundqvist, Svalöf Weibull AB, SE-268 81 Svalöv, Sweden. FAX: +46 418 667109; e-mail: <udda@ngb.se>
Biochemical mutants - Including lysine, hordein and nitrate reductase: Andy Kleinhofs, Department of Crop and Soil Sciences, Washington State University, Pullman, WA 99164-6420, USA. FAX: +1 509 335 8674; e-mail: <andyk@wsu.edu>
Barley-wheat genetic stocks: A.K.M.R. Islam, Department of Plant Science, Waite Agricultural Research Institute, The University of Adelaide, Glen Osmond, S.A. 5064, Australia. FAX: +61 8 8303 7109; e-mail: <rislam@waite.adelaide.edu.au>
Disease and pest resistance genes: Brian Steffenson, Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN 55108-6030, USA. FAX: +1 612 625 9728; e-mail: <bsteffen@umn.edu>
Coordinator’s Report: Barley Chromosome 1H (5)
Gunter Backes
Risø National Laboratory, Plant Research Department
Resistance Biology Programme PRD 339
P.O. Box 49, DK-4000 Denmark
Long et al., 2003 performed a marker regression based on a segregating population of 110 doubled haploid lines, derived from the cross ‘Mundah/Keel’. Grain yield and early dry matter production were associated with the chromosome 1H fragment containing the SSR markers Bmac32 and Ebmac501. For the chromosome 1H fragment with the AFLP markers P13/M49-251 and the SSR marker Awbma35, they found associations with growth habit, early maturity, kernel yield, kernel weight and kernel screening fractions.
Read et al., 2003 localised a QTL for heading date on chromosome 1H in 166 DH lines from a cross between the varieties ‘Sloop’ and ‘Halicon’. The QTL was localised between the SNP locus SOUISC4 and the microsatellite locus Bmac0382. The marker explained 28 % of the phenotypic variance.
Pillen et al., 2003 searched for associations between microsatellite markers and agronomic traits in 136 BC2F2 lines from an advanced backcross involving the wild barley (Hordeum vulgare ssp. spontaneum) line ‘101-23’ and the barley variety ‘Apex’. They found the following associations as expression of putative QTLs on chromosome 1H: an association between the SSR marker GMS21 and heading date, kernels per ear and kernel yield, an association between heading date and the SSR marker Bmac0213, an association between the traits heading date, plant height and lodging with the microsatellites HvALAAT, HVM20 and HVM64 and finally between the same traits and Bmag0211. For all QTLs, apart from the kernel yield related ones mentioned first, the alleles from the wild line were more favourable in relation to common breeding goals.
Mickelson et al., 2003 studied nitrogen storage and remobilization in barley leaves in a RI-population based on a cross between the two barley varieties ‘Karl’ and ‘Lewis’. On chromosome 1H, two regions of interest were detected: one QTL for “total leaf nitrogen at mid-grain fill” near the RFLP marker ABG53 and one QTL near the AFLP marker TB2122 for the traits “total leaf nitrogen at anthesis”, “total leaf nitrogen at maturity” and yield.
Teulat et al., 2003 found a locus with QTL x environment interaction for relative water content near the locus for black pericarp colour (bpc) on the long arm of chromosome 1H. The localisation was carried out in 167 recombinant inbred lines (RILs) from the cross ‘Tadmor/Er-Apm’, and based on data of humid as well as drought environments.
Also targeting towards genes of interest for barley cultivation under drought conditions, Baum et al., 2003 were localising QTL for agronomic traits under mild and heavy drought stress. The analysis was based on 194 RIL lines from a cross between the ICARDA-variety ‘Arta’ and a Spontaneum line. Five QTLs were found on chromosome 1H: one QTL near the SSR marker Bmag0105 for biological yield and plant height, on just beside for growth habit and kernel weight, but only under favourite conditions, one for biological yield and tiller number, one for growth habit and finally one QTL for kernel weight, biological yield and tiller number.
In 150 DH lines from ‘Steptoe/Morex’, Han et al., 2003 localised a QTL for acid detergent fibre (ADF). The locus was detected in the interval between the markers AGA006 and Hor2 and accounted for 23.6 % of the variation for this trait.
Three QTLs for resistance against Fusarium head blight on chromosome 1H were localised by Dahleen et al., 2003 in a DH population(75 lines) resulting from the three-way cross ‘Zhedar 2/ND9712//Foster’. Those three QTLs were environment-specific.
In an attempt to analyse the relation of stoma density and “avoidance” against Puccinia hordei, Vaz Patto et al., 2003 localised QTLs for the respective QTLs in a population of 100 F2 plants derived from a cross between two Hordeum chilense accessions. On chromosome 1H, they detected a QTL for “avoidance”. There was no correlation between stoma density and “avoidance”.
Madsen et al., 2003 described the development and localisation of Resistance Gene Analogues (RGAs) for barley. On chromosome 1H, three RGAs were localized: two (S-9217 and S-112, both between the RFLP locus MWG55 and the SSR locus Bmag0211) in the mapping population ‘1B-87/Vada’ (Backes, et al., 2003) and one (S-9240B, between the RFLP markers PSB67 and WG518) in the mapping population ‘Igri/Triumph’ (Laurie et al., 1995).
In the above mentioned mapping population derived from a cross between the Spontaneum-line ‘1B-87’ and the barley variety ‘Vada’ (121 RI lines), Backes et al,. 2003 localised a QTL for quantitative resistance against powdery mildew (caused by Blumeria graminis) acting additively in the field experiment at or nearby the Mla locus conferring qualitative resistance against the same disease.
Collins et al. used four different mapping populations for the localisation of QTLs for malt extract: a DH-population from the cross ‘Sloop/Alexis’ (Barr et al., 2003), a RI-population from the cross ‘Sloop-sibling/Alexis’ (Barr et al., 2003), a DH-population from the cross ‘Galleon/Haruna Nijo’ (Karakousis et al., 2003) and a DH-population from the cross ‘Chebec/Harrington’ (Barr et al., 2003). On chromosome 1H, they detected two QTLs: one near the centromere in the mapping populations Sloop/Alexis’ (near the microsatellite locus Ebmac0501) and ‘Sloop-sibling/Alexis’ and one on the long arm in ‘Chebec/Harrington’ (near the RFLP locus BCD508).
Clancy et al., 2003 localised QTLs for beta-amylase activity simultaneously in the three segregating populations derived from the crosses ‘Steptoe/Morex’, ‘TR306/Harrington’ and ‘Harrrington/Morex’, ranging from 144 to 150 doubled haploid (DH) lines. In two of the three crosses, they found a major QTL for beta-amylase activity on the short arm of chromosome 1H close by the Hor1 locus. An additional minor QTL for beta-amylase activity and diastatic power was detected on the same chromosome, but only in ‘Steptoe/Morex’.
In a DH population of 65 lines from the cross ‘Tallon/Kaputar’, Cakir et al., 2003 localised a QTL for diastatic power on chromosome 1H.
References:
Backes, G., L.H. Madsen, H. Jaiser, J. Stougaard, M. Herz, V. Mohler and A. Jahoor. 2003. Localisation of genes for resistance against Blumeria graminis f. sp. hordei and Puccinia graminis in a cross between a barley cultivar and a wild barley (Hordeum vulgare ssp. spontaneum) line. Theor. Appl. Genet. 106:353-362.
Barr, A.R., A. Karakousis, R.C.M. Lance, S.J. Logue, S. Manning, K.J. Chalmers, J.M. Kretschmer, J.R. Boyd, H.M. Collins, S. Roumeliotis, S.J. Coventry, D.B. Moody, B.J. Read, D. Poulsen, C.D. Li, G.J. Platz, P.A. Inkerman, J.F. Panozzo, B.R. Cullis, D.B. Smith, P.Lim and P. Langridge. 2003. Mapping and QTL analysis of the barley population Chebec × Harrington. Aust. J. Agr. Res. 54:1125-1130.
Baum, M., S. Grando, G. Backes, A. Jahoor, A. Sabbagh and S. Ceccarelli. 2003. QTLs for agronomic traits in the Mediterranean environment identified in recombinant inbred lines of the cross 'Arta' × H. spontaneum 41-1. Theor. Appl. Genet. 107:1215-1225.
Cakir, M., D. Poulsen, N.W. Galwey, G.A. Ablett, K.J. Chalmers, G.J. Platz, R.C.M. Lance, J.F. Panozzo, B.J. Read, D.B. Moody, A.R. Barr, P. Johnston, C.D. Li, W. J.R. Boyd, C R. Grime, R. Apples, M.G.K. Jones and P. Langridge. 2003. Mapping and QTL analysis of the barley population Tallon × Kaputar. Aust. J. Agr.Res. 54:1155-1162.
Clancy, J.A., F. Han and S.E. Ullrich. 2003. Comparative mapping of beta-amylase activity QTLs among three barley crosses. Crop Sci. 43:1043-1052.
Dahleen, L.S., R. Horsley, B.J. Steffenson, P.B. Schwarz, A. Mesfin and J.D. Franckowiak. 2003. Identification of QTLs associated with Fusarium head blight resistance in Zhedar 2 barley. Theor. Appl. Genet. 108:95-104.
Han, F., S.E. Ullrich, I. Romagosa, J.A. Clancy, J.A. Froseth and D.M. Wesenberg. 2003. Quantitative genetic analysis of acid detergent fibre content in barley grain. J. Cereal Sci. 38:167-172.
Karakousis, A., A.R. Barr, J.M. Kretschmer, S. Manning, S.J. Logue, S.J. Logue, C.D. Li, R.C.M. Lance and P. Langridge. 2003. Mapping and QTL analysis of the barley population Galleon × Haruna Nij. Aust. J. Agr. Res. 54:1131-1135.
Laurie, D.A., N. Pratchett, J.H. Bezant and J. W. Snape. 1995. RFLP mapping of 5 major genes and 8 quantitative trait loci controlling flowering time in a winter × spring barley (Hordeum vulgare L.) cross. Genome 38:575-585
Long, N.R., S.P. Jefferies, A. Karakousis, J.M. Kretschmer, C. Hunt., P. Lim, P.J. Eckermann and A. R. Barr. 2003. Mapping and QTL analysis of the barley population Mundah × Keel. Aust. J. Agr. Res. 54:1163-1171.
Madsen, L.H., N.C. Collins, M. Rakwalska, G. Backes, N. Sandal, L. Krusell, J. Jensen, E. H. Waterman, A. Jahoor, M. Ayliffe, A.J. Pryor, P. Langridge, P. Schulze-Lefert and J. Stougaard. 2003. Barley disease resistance gene analogs of the NBS-LRR class: identification and mapping. Mol. Gen. Genet. 269:150-161.
Mickelson, S., D. See, F.D. Meyer, J.P. Garner, C.R. Foste, T.K. Blake and A.M. Fischer. 2003. Mapping of QTL associated with nitrogen storage and remobilization in barley (Hordeum vulgare L.) leaves. J. Exp. Bot. 54:801-812.
Pillen, K., A. Zacharias and J. Léon. 2003. Advanced backcross QTL analysis in barley (Hordeum vulgare L.). Theor. Appl. Genet. 107:340-352.
Read, B.J., H. Raman, G. McMichael, K.J. Chalmers, G.A. Ablett, G.J. Platz, R. Raman, R. K.Genger, J.R. Boyd, C.D. Li, C.R. Grime, R.F. Park, H. Wallwork, R. Prangnell and R.C.M. Lance. 2003. Mapping and QTL analysis of the barley population Sloop × Halcyon. Aust. J. Agr. Res. 54:1145-1153.
Teulat, B., N. Zoumarou-Wallis, B. Rotter, M. Ben Salem and D. This. 2003. QTL for relative water content in field-grown barley and their stability across Mediterranean environments. Theor. Appl. Genet. 108:181-188.
Vaz Patto, M., D. Rubiales, A. Martín, P. Hernàndez, P. Lindhout, R.E. Niks and P. Stam. 2003. QTL mapping provides evidence for lack of association of the avoidance of leaf rust in Hordeum chilense with stomata density. Theor. Appl. Genet. 106:1283-1292.
Coordinator’s report: Chromosome 2H (2)
J.D. Franckowiak
Department of Plant Sciences
North Dakota State University
Fargo, ND 58105, U.S.A.
Dahleen et al., 2003 and Mesfin et al. 2003 published papers on the inheritance of resistance to Fusarium head blight (FHB), incited primarily by Fusarium graminearum Schwabe), in crosses between two- and six-rowed barley. Quantitative trait loci (QTL) for FHB resistance were again reported to occur in chromosome 2H. The reports identified three QTLs for FHB resistance and two coincident QTLs for deoxynivalenol (DON) accumulation in chromosome 2H. One QTL is near the vrs1 (six-rowed spike 1) locus and another is near the Eam6 (early maturity 6) locus. The late two-rowed parents had QTLs for FHB resistance. Mesfin et al., 2003 reported that the largest heading date effect associated with the Eam6 gene was observed in a fall greenhouse nursery.
Krasheninnik and Franckowiak, 2003 studied that FHB resistance in the Harrington/Morex (HM) doubled-haploid population and found the largest QTL for FHB resistance in chromosome 2H. The map developed for the HM population by Marquez-Cedillo et al., 2001 was used in the analysis of data. A QTL for early heading in China, a short-day environment, was at the same position in chromosome 2H as the heading date QTL reported for long-day response. This suggests that Eam6 influences heading date in both long- and short-day environments. A preliminary report by Franckowiak et al., 2003 suggests that Eam6 is ineffective in New Zealand where days are slightly longer than 12 hours at planting.
Tanno et al., 2002 used molecular marker cMWG699, which is very close (01. cM) to the vrs1 locus, to study the origins of cultivated six-rowed barley. Based on marker differences, they divided six-rowed barleys into two distinct groups, types I and II. Type I is widely distributed while Type II is found only in the Mediterranean region. Since both types exist among two-rowed barley cultivars, six-rowed barley probably originated from at least two independent mutations at the vrs1 locus.
Ayoub et al., 2002 studied kernel size and shape in the HM doubled-haploid population. They found a large QTL for kernel size associated with the vrs1 locus. The two-rowed cultivar, Harrington, had larger kernels than the six-rowed cultivar, Morex. Similar results on 1000-kernel weights were reported by Hori et al., 2003 using another two- by six-rowed population of F9 recombinant inbred lines. These results with previous studies that found pleiotropic effects of vrs1 alleles on kernel size.
Weerasena et al., 2003 reported on the conversion of amplified fragment length polymorphism (AFLP) marker P13M40 to a co-dominant marker for Rph15 (reaction to Puccinia hordei 15) locus in chromosome 2HS. This gene conferred resistance all expect one isolate in a collection of over 350 P. hordei isolates (Fetch et al., 1998). The leaf rust resistance gene Rph15.ad was shown to be an allele of the gene Rph16.ae, which was identified by Ivandic et al., 1998 in wild barley (Hordeum vulgare ssp. spontaneum).
Backes et al., 2003 identified a QTL for resistance to powdery mildew (Blumeria graminis f. sp. hordei) in chromosome 2HS and a QTL for leaf rust (Puccinia hordei) resistance in chromosome 2HL. The study was conducted using the progeny of a cross between ‘Vada’ and wild barley accession 1B-87 from Israel.
Decousset et al., 2000 reported on the development of sequence tagged site (STS) primer pairs for the Ppd-H1 or Eam1 locus in chromosome 2HS. Plants with the Eam1 gene are very early when grown under long-day conditions (Tohno-oka et al., 2000).
Canci et al., 2003 identified a minor QTL for kernel discoloration in chromosome 2H. However, two major QTLs for kernel color were found in chromosome 6H and one of these was coincident with a major QTL for high grain protein from ‘Chevron’.
Li et al., 2003 mapped 127 new microsatellite markers in barley. One of the four large clusters of makers that they found was in chromosome 2H.
Arru et al., 2003 mapped a QTL for resistance to leaf stripe (Pyrenophora graminea) in chromosome 2H of ‘Steptoe’. The QTL is at a different position in chromosome 2H than the Rdg1 locus, which also confers resistance to leaf stripe of barley.
References:
Arru, L., E. Francia, and N. Pechioni. 2003. Isolate-specific QTLs of resistance to leaf stripe (Pyrenophora graminea) in the ‘Steptoe’ X ‘Morex” spring barley cross. Theor. Appl. Genet. 106:668-675.
Ayoub, M. S.J. Symons, M.J. Edney, and D.E. Mather. 2002. QTLs affecting kernel size and shape in a two-rowed by six-rowed barley cross. Theor. App. Genet. 105:237-247.
Backes, G., L.H. Madsen, H. Jaiser, J. Stougaard, M. Herz, V. Mohler, and A. Jahoor. 2003. Localisation of genes for resistance against Blumeria graminis f. sp. hordei and Puccinia graminis in a cross between a barley cultivar and a wild barley (Hordeum vulgare ssp. spontaneum) line. Theor. Appl. Genet. 106:353-362.
Canci, P.C., L.M. Nduulu, R. Dill-Macky, G.J. Muehlbauer, D.C. Rasmusson, and K.P. Smith. 2003. Genetic relationship between kernel discoloration and grain protein concentation in barley. Crop Sci. 43:1671-1679.
Dahleen, L.S., H.A. Agrama, R.D. Horsley, B.J. Steffenson, P.B. Schwarz, A. Mesfin, and J.D. Franckowiak. 2003. Identification of QTLs associated with Fusarium head blight resistance in Zhedar 2. Theor. Appl. Genet. 108:95-104.
Decousset, L., S. Griffiths, R.P. Dunford, N. Pratchett, and D.A. Laurie. 2000. Development of STS markers closely linked to the Ppd-H1 photoperiod response gene in barley (Hordeum vulgare L.). Theor. Appl. Genet. 101:1202-1206.
Fetch, T,G. Jr., B.J. Steffenson, and Y. Jin. 1998. Worldwide virulence of Puccinia hordei on barley. Phytopathology 88:528.
Franckowiak, J.D., G. Yu, and N. Krasheninnik. 2003. Genetic control of photoperiod responses in spring barley. p. 98. In Proc. 3rd Canadian Barley Symposium, 19 and 20 June, 2003, Red Deer, Alberta, Canada.
Hori, K., T. Kobayashi, A. Shimizu, K. Sato, K. Takeda, and S. Kawasaki. 2003. Efficient construction of high-density linkage map and its application to QTL analysis in barley. Theor. Appl. Genet. 107:806-813.
Ivandic, V., U. Walther, and A. Graner. 1998. Molecular mapping of a new gene in wild barley conferring complete resistance to leaf rust (Puccinia hordei Otth). Theor. Appl. Genet. 97:1235-1239.
Krasheninnik, N.N., and J.D. Franckowiak. 2003. Identification of QTLs in the Harrington/Morex barley population for FHB reaction, maturity, and plant height. p. 260-263. In S. Canty, J. Lewis, and R.W. Ward (eds.) Proc. National Fusarium Head Blight Forum, 2003 Dec 13-15; Bloomington, MN. Michigan State University, East Lansing.
Li, J.Z., T.G. Sjakste, M.S. Röder, and M.W. Ganal. 2003. Development and genetic mapping of 127 new microsatellite markers in barley. Theor. Appl. Genet. 107:1021-1027.
Marquez_Cedillo, L.A., P.M. Hayes, A. Kleinhofs, W.G. Legge, B.G. Rossnagel, K. Sato, S.E. Ullrich, and D. M. Wesenberg. 2001. QTL analysis of agronomic traits in barley based on the doubled haploid progeny of two elite North American varieties representing different germplasm groups. Theor. Appl. Genet. 103:625-637.
Mesfin, A. K.P. Smith, R. Dill-Macky, C.K. Evans, R. Waugh, C.D. Gustus, and G.J. Muehlbauer. 2003. Quantitative trait loci for Fusarium head blight resistance in barley detected in a two-rowed by six-rowed population. Crop Sci. 43:307-318.
Tanno, K., S. Taketa, K. Takeda, and T. Komatsuda. 2002. A DNA marker closely linked to the vrs1 locus (row-type gene) indicates multiple origins of six-rowed cultivated barley (Hordeum vulgare L.). Theor. Appl. Genet. 104:54-60.
Tohno-oka, T., M. Ishit, R. Kanatani, H. Takahashi, and K. Takeda. 2000. Genetic analysis of photoperiodic response of barley in different daylength conditions. p. 239-241. In S. Logue (ed.) Proc. Eight Int. Barley Genet. Symp., Volume III. Adelaide University, Glen Osmond, South Australia.
Weerasena, J.S., B.J. Steffenson, and A.B. Falk. 2003. Conversion of an amplified fragment length polymorphism marker into a co-dominant marker in mapping of the Rph15 gene conferring resistance to barley leaf rust, Puccinia hordei Otth. Theor. Appl. Genet., in press.
Coordinators Report: Barley Chromosome 5H (7)
George Fedak
Eastern Cereal & Oilseed Research Centre
Agriculture & Agri-Food Canada
Ottawa, Ontario, K1A 0C6
The seed dormancy loci SD1 and SD2 of Steptoe barley have previously been mapped to chromosome 7 (5H) (Han et al., 1996). SD2 had been mapped to a 8cM interval between markers ABC 309 and MWG851 at the distal end of the long arm (Ullrich et al., 1996). This locus was subsequently fine mapped to a 0.8cM interval using a substitution mapping approach. The flanking markers were MWG 851D and MWG 851B (Gao et al., 2003). There was probably another dormancy QTL in the ABG 496 - MWG 851C interval of 5H that reduced the dormancy effect of SD2.
The locus Ba YMV/Ba YMV-2, that provides resistance to the two strains of barley yellow mosaic virus from the barley variety Chikurin Ibaraki was mapped to chromosome 5H. Three SSR markers, Bmac 0306, Bmac 0163 and Bmac 0113 cosegrated with the resistance locus. It was concluded that the resistance locus is included in a 4.3cM interval spanned by the three markers (Werner et al., 2003).
It is interesting to note that the barley yellow mosaic resistance locus rym3 has previously been mapped to chromosome 5H (Saeki et al., 1999). The resistance locus was obtained from barley line Ea52. The latter is a gamma ray induced mutant of the variety Chikurin Ibaraki.
References:
Gao, W., J.A. Clancy, F. Han, D. Prada, A. Kleinhofs and S.E. Ullrich. 2003. Molecular dissection of a dormancy QTL region near the chromosome 7 (5H) L Telomere in barley. Theor. Appl. Genet. 107:552-559.
Han, F., S.E. Ullrich, J.A. Clancy and I. Ramagosa, 1999. Inheritance and fine mapping of a major barley seed dormancy QTL. Plant Sci. 143:113-118.
Saeki, K., C. Miyazaki, N. Hirota, A. Saito, K. Ito and T. Konishi, 1999. RFLP mapping of BaYMV resistance gene rym3 in barley (Hordeum vulgare). Theor. Appl. Genet. 99:727-732.
Ullrich, S.E., F. Han, T.K. Blake, L.E. Oberthur, W.E. Dyer and J.A. Clancy, 1996. Seed dormancy in barley. Genetic resolution and relationships to other traits. pp:157-163. In: Noda, K. and Mares, D.J. (eds.) Preharvest sprouting in cereals 1995. Center for Academic Societies, Osaka, Japan, Proc.7th International Symposium on Pre-Harvest Sprouting in Cereals held at Abashiri, Hokkaido, Japan.
Werner, K., W. Friedt, E. Laubach, R. Waugh and F. Ordon. 2003. Dissection of resistance to soil-borne yellow-mosaic-inducing viruses of barley (BaMMV, BaYMV, BaYMV-2) in a complex breeders=s cross by means of SSRs and simultaneous mapping of BaYMV/BaYMV-2 resistance of var. ‘Chikurin Ibaraki 1’. Theor Appl. Genet. 106:1425-1432.
Coordinator’s report: Chromosome 7H
Lynn S. Dahleen
USDA-Agricultural Research Service
Fargo, ND 58105, USA
Three studies examined polymorphisms among various genotype collections. Russell et al., 2003 sampled landraces from Syria and Jordan and tested polymorphism with 21 SSRs. One of the chromosome 7H SSRs tested showed three alleles while the other SSR locus had 31 different alleles in the 125 landrace accessions genotyped. Sjakste et al., 2003 examined microsatellite allele inheritance in the 37 European ancestors of seven Latvian varieties. The 14 SSRs tested from chromosome 7H two to seven alleles, and an average polymorphism information content (PIC) value of 0.62, a bit above the overall PIC average of 0.57. Allelic pedigrees were constructed to trace the genetic route of each current allele back to the specific ancestral source. Lund et al., 2003 used SSRs to evaluate potential duplicate groups in gene bank collections. The 35 primer pairs tested, six from chromosome 7H, identified 22 homogeneous groups among the 36 groups studied, providing a rapid method for identifying duplicates.
Pillen et al., 2003 conducted a QTL analysis of a BC2F2 population between an H. v. spontaneum and the recurrent spring barley Apex. QTLs for heading date, harvest index, malt tenderness, yield, height, thousand-grain weight, water absorption, and above ground biomass were located on chromosome 7H. Many of the favorable alleles for these important traits were from H. v. spontaneum. Matus et al., 2003 developed recombinant chromosome substitution lines by backcrossing an H. vulgare subsp. spontaneum accession to Harrington barley. They then used 47 SSR markers to determine the percentage of spontaneum introgressed into the lines. The average length of the donor segment in chromosome 7H was 39.0 cM, with segregation distorted towards the donor’s DNA. Baum et al., 2003 tested recombinant inbred lines from another H. v. spontaneum cross, with Arta. They developed a linkage map on 189 markers, including 24 on chromosome 7H. QTLs located on this chromosome included those for grain yield, 1000-kernel weight, days to heading, plant height, beta-glucan content, biological yield, and cold damage. Only the QTL for cold damage was significant in more than one environment. SSRs were used with genomic in situ hybridization (GISH) to evaluate wheat x barley backcross-derived lines (Malysheva et al., 2003). One BC1 plant contained only two small 7H fragments which were not transmitted to the progeny. The other plant contained the complete chromosome 7H. Only the end of the short arm was detected in BC2 progeny. Similar patterns of elimination were observed for other chromosomes.
Several groups reported on the development of additional markers. Li et al., 2003 developed 127 new SSR markers from genomic clones. Nine of the SSRs were located on chromosome 7H. Thiel et al., 2003 developed 76 new SSR markers from EST database information; seven were on chromosome 7H. Kota et al., 2003 also used EST collections to identify single nucleotide polymorphisms (SNPs) in barley. Of the 28 SNPs mapped in this study, three were on chromosome 7H. Another type of marker, based on NBS-LRR class resistance gene analogs (RGAs), was developed and mapped by Madsen et al., 2003. Three of these RGAs mapped to chromosome 7H.
Placement of morphological markers on the molecular maps continued. Pozzi et al., 2003 mapped 29 developmental mutants using RFLP-AFLP techniques. Both the lks2 gene for short awns and the sld4 gene for slender dwarf 4 were placed on the Proctor x Nudinka AFLP map of chromosome 7H. Dahleen et al., 2003 identified linkages between SSR markers on chromosome 7H and the lks.o and bra-a.001 morphological genes. Kikuchi et al., 2003 fine-mapped the naked caryopsis gene, nud, using bulked segregant analysis and AFLP markers. The nud locus was mapped within a 1.5 cM region and cosegregated with two AFLP markers. Their data show that nud is further from the centromere than previously reported.
Gene mapping reports also included those involved in disease response. Chen et al., 2003 compared genomic locations of rice and barley QTLs for resistance to rice blast. The 12 QTLs in barley included three on chromosome 7H. One of these loci was syntenic to a locus on rice chromosome 8. One of the QTL maps for Fusarium head blight (FHB), developed by Mesfin et al., 2003, identified several FHB QTLs, but only detected one small QTL for resistance in the greenhouse on chromosome 7H. Rostoks et al., 2003 isolated and mapped genes homologous to maize hypersensitive-induced reaction (HIR) genes. Hv-hir4 was located on the short arm of chromosome 7H in bin 03. This genes was similar to the other three HIR genes at the amino acid level, but not at the sequence level.
QTL analysis of recombinant inbred lines from a 2-rowed by 6-rowed spring barley cross was conducted using a map developed using the high efficiency genome scanning system (Hori et al., 2003). This system allowed map construction in six months, and QTLs for plant height and spike exertion were located on chromosome 7H. Teulat et al., 2003 located QTLs for relative water content in field-grown barley. One QTL representing main and QTL x E effects and one QTL that was detected only in one environment were located on chromosome 7H. Clancy et al., 2003 conducted comparative mapping of beta-amylase activity QTLs among the three mapping populations from the North American Barley Genome Project. Both populations involving Morex showed coincident QTLs for beta amylase and diastatic power on chromosome 7H. This region contains several other QTL for malting quality. QTLs for kernel discoloration and grain protein content were located by Canci et al., 2003. Two QTLs for kernel discoloration located on chromosome 7H were detected in a single environment, explaining 6.4-10.1 % of the variation for this trait. No regions on this chromosome were associated with grain protein content.
References:
Baum, M., S. Grando, G. Backes, A. Jahoor, A. Sabbagh, and S. Ceccarelli. 2003. QTLs for agronomic traits in the Mediterranean environment identified in recombinant inbred lines of the cross ‘Arta’ x H. spontaneum 41-1. Theor. Appl. Genet. 107:1215-1225.
Canci, P.C., L.M. Nduulu, R. Dill-Macky, G.M. Muehlbauer, D.C. Rasmusson, and K.P. Smith. 2003. Genetic relationships between kernel discoloration and grain protein concentration in barley. Crop Sci. 43:1671-1679.
Chen, H., S. Wang, Y. Xing, C. Xu, P.M. Hayes, and Q. Zhang. 2003. Comparative analyses of genomic locations and race specificities for quantitative resistance to Pyricularia grisea in rice and barley. Proc. Natl. Acad. Sci. USA 100:2544-2549.
Clancy, J.A., F. Hans, S.E. Ulrich, and the North American Barley Genome Project. 2003. Comparative mapping of B-amylase activity QTLs among three barley crosses. Crop Sci. 43:1043-1052.
Dahleen, L.S., J.D. Franckowiak, and L.J. Vander Wal. 2003. Exposing students and teachers to plant molecular genetics with short-term barley gene mapping projects. J. Nat. Resour. Life Sci. Educ. 32:61-64.
Hori, K., R. Kobayashi, A. Shimizu, K. Sato, K. Takeda, and S. Kawasaki. 2003. Efficient construction of high-density linkage map and its application to QTL analysis in barley. Theor. Appl. Genet. 107-806-813.
Kikuchi, S., S. Taketa, M. Ichii, and S. Kawasaki. 2003. Efficient fine mapping of the naked caryopsis gene (nud) by HEGS (High Efficiency Genome Scanning) /AFLP in barley. Theor. Appl. Genet. 108:73-78.
Kota, R., S. Rudd, A. Facius, G. Kolesov, T. Thiel, H. Zhang, N. Stein, K. Mayer, and A. Graner. 2003. Snipping polymorphisms from large EST collections in barley (Hordeum vulgare L.). Mol. Gen. Genomics 270:24-33.
Li, J.Z., T.G. Sjakste, M.S. Roder, and M.W. Ganal. 2003. Development and genetic mapping of 127 new microsatellite markers in barley. Theor. Appl. Genet. 107:1021-1027.
Lund, B., R. Oritz, I.M. Skovgaard, R. Waugh, and S.B. Andersen. 2003. Analysis of potential duplicates in barley gene bank collections using re-sampling of microsatellite data. Theor. Appl. Genet. 106:1129-1138.
Madsen, L.H., N.C. Collins, M. Rakwalska, G. Backes, N. Sandal., L. Krusell, J. Jensen, E.H. Waterman, A. Jahoor, M. Ayliffe, A.J. Pryor, P. Langridge, P. Schulze-Lefert, and J. Stougaard. 2003. Barley disease resistance gene analogs of the NBS-LRR class: identification and mapping. Mol. Gen. Genomics 269:150-161.
Malysheva, L., T. Sjakste, F. Matzk, M. Roder, and M. Ganal. 2003. Molecular cytogenetic analysis of wheat-barley hybrids using genomic in situ hybridization and barley microsatellite markers. Genome 46:413-322.
Matus, I., A. Corey, T. Filichkin, P.M. Hayes, M.I. Vales, J. Kling, O. Riera-Lizarazu, K. Sato, W. Powell, and R. Waugh. 2003. Development and characterization of recombinant chromosome substitution lines (RCSLs) using Hordeum vulgare subsp. spontaneum as a source of donor alleles in a Hordeum vulgare subsp. vulgare background. Genome 46:1010-1023.
Mesfin, A., K.P. Smith, R. Dill-Macky, C.K. Evans, R. Waugh, C.D. Gustus, and G. Muehlbauer. 2003. Quantitative trait loci for Fusarium head blight resistance in barley detected in a two-rowed by six-rowed population. Crop Sci. 43: 307-318.
Pillen, K., A. Zacharias, and J. Leon. 2003. Advanced backcross QTL analysis in barley (Hordeum vulgare L.). Theor. Appl. Genet. 107:340-352.
Pozzi, C., D. di Pietro, G. Halas, C. Roig, and F. Salamini. 2003. Integration of a barley (Hordeum vulgare) molecular linkage map with the positions of genetic loci hosting 29 developmental mutants. Heredity 90:390-396.
Rostoks, N., D. Schmierer, D. Kudrna, and A. Kleinhofs. 2003. Barley putative hypersensitive induced reaction genes: genetic mapping, sequence analysis and differential expression in disease lesion mimic mutants. Theor. Appl. Genet. 107:1094-1101.
Russell, J.R., A. Booth, J.D. Fuller, M. Baum, S. Ceccarelli, S. Grando, and W. Powell. 2003. Patterns of polymorphism detected in the chloroplast and nuclear genomes of barley landraces sampled from Syria and Jordan. Theor. Appl. Genet. 107:413-421.
Sjakste, T.G., I. Rashal, and M.S. Roder. 2003. Inheritance of microsatellite alleles in pedigrees of Latvian barley varieties and related European ancestors. Theor. Appl. Genet. 106:539-549.
Teulat, B., N. Zoumarou-Wallis, B. Rotter, M. Ben Salem, H. Bahri, and D. This. 2003. QTL for relative water content in field-grown barley and their stability across Mediterranean environments. Theor. Appl. Genet. 108:181-188.
Thiel, T., W. Michalek, R.K. Varshney, and A. Graner. 2003. Exploiting EST databases for the development and characterization of gene-derived SSR markers in barley (Hordeum vulgare L.). Theor. Appl. Genet. 106:411-422.
Integrating Molecular and Morphological/Physiological Marker Maps
A. Kleinhofs
Dept. Crop and Soil Sciences and
School of Molecular Biosciences
Washington State University
Pullman, WA 99164-6420, USA
During the past year I have attempted to integrate the Morphological /Physiological/ Disease resistance markers into the molecular Bin map. The results were sent to GrainGenes to produce an integrated map. My goal is to produce an interactive map that would incorporate pictures of markers that are “photogenic”, decriptions of markers, high resolution maps where available, BAC clone addresses, etc. The results can be viewed at http://ceres.plbr.cornell.edu/cgi-bin/gbrowse. This is obviously a work in progress, but to date it is not very viewer friendly or easy to navigate through, but the information is there. For those who would prefer the information in a less cumbersome form I reproduce it here, minus the pictures and high resolution maps, of course.
Please advise me if you have additions or corrections to this information.
Bin Assignments for Morphological Map Markers and closest molecular marker
Chr.1(7H)
BIN1 Rpg1 RSB228 Brueggeman et al., PNAS 99:9328, ‘02
Run1
Rdg2a MWG851A Bulgarelli et al., TAG 108:1401-1408 ,’04
Rrs2 MWG555A Schweizer et al., TAG 90:920, ‘95
mlt
brh1 MWG2074B Li et al., 8th IBGS 3:72, ‘00
BIN2 Est5 iEst5 Kleinhofs et al., TAG 86:705, ‘93
wax Wax Kleinhofs BGN32:152, ‘02
gsh3 His3A Kleinhofs BGN32:152, ‘02
BIN3 fch5 ABC167A Kleinhofs BGN32:152, ‘02
Rcs5 KAJ185 Johnson & Kleinhofs, unpublished
yvs2
cer-ze ABG380Kleinhofs BGN27:105, ‘96
BIN4 wnd
Lga BE193581 Johnson & Kleinhofs, unpublished
abo7
BIN5 ant1
nar3 MWG836 Kleinhofs BGN32:152, ‘02
ert-m
ert-a
BIN6 ert-d
fch8
fst3
cer-f
dsp1
msg14
BIN7 msg10
rsm1 BC455 Edwards & Steffenson, Phytopath. 86:184,’96
sex6
seg5
seg2
pmr ABC308 Kleinhofs BGN27:105, ‘96
mo6b Hsp17 Soule et al., J Her. 91:483, ’00
nud CDO673 Heun et al., Genome 34:437, ‘91
fch4 MWG003 Kleinhofs BGN27:105, ‘96
BIN8 Amy2 Amy2 Kleinhofs et al., TAG 86:705, ‘93
lks2 WG380B Costa et al., TAG 103:415, ‘01
Rpt4 Psr117D Williams et al., TAG 99:323, ‘99
ubs4
blx2
BIN9 lbi3
xnt4
lpa2 ? Larson et al., TAG 97:141, ‘98
msg50
Rym2
seg4
BIN10 Xnt1 BF626025 Hansson et al., PNAS 96:1744, ‘99
xan-h BF626025 Hansson et al., PNAS 96:1744, ‘99
BIN11Rph3 Tha2 Toojinda et al., TAG 101:580, ‘00
BIN12Mlf
xnt9
seg1
msg23
BIN13none
BIN14none
Chr.2(2H)
BIN1 sbk
BIN2 none
BIN3 gsh6 MWG878A Kleinhofs BGN32:152, ‘02
gsh1
gsh8
BIN4 Eam1
Ppd-H1 MWG858 Laurie et al., Heredity 72:619, ‘94
sld2
rtt
flo-c
sld4
BIN5 fch15
brc1
com2
BIN6 msg9
abo2
rph16 MWG874 Drescher et al., 8thIBGS II:95, ‘00
BIN7 yst4 CDO537 Kleinhofs BGN32:152, ‘02
Az94 CDO537 Kleinhofs BGN32:152, ‘02
gaiMWG2058 Börner et al., TAG 99:670, ‘99
msg33
msg3
fch1
BIN8 Eam6 ABC167b Tohno-oka et al., 8thIBGS III:239, ‘00
gsh5
msg2
eog ABC451 Kleinhofs BGN27:105, ‘96
abr
cer-n
BIN9 Gth
hcm1
wst4
vrs1 MWG699 Komatsuda et al., Genome 42:248, ‘00
BIN10cer-g
Lks1
mtt4
Pre2
msg27
ant2
BIN11Rha2 AWBMA21 Kretschmer et al., TAG 94:1060, ‘97
Rar1 AW983293B Freialdenhoven et al., Plt. Cell 6:983, ’94
fol-a
galMWG581A Börner et al., TAG 99:670, ‘99
fch14
Pau
BIN12Pvc
BIN13 lig BCD266 Pratchett & Laurie Hereditas 120:35, ‘94
nar4 Gln2 Kleinhofs BGN27:105, ‘96
Zeo1 cnx1 Costa et al., TAG 103:415, ‘01
lpa1 ABC157 Larson et al., TAG 97:141, ‘98
BIN14none
BIN15gpa CDO036 Kleinhofs BGN27:105, ‘96
wst7 MWG949A Costa et al., TAG 103:415, ‘01
MlLa Ris16 Giese et al., TAG 85:897, ‘93
trp
Chr. 3(3H)
BIN1 Rph5
Rph6
Rph7 MWG848 Brunner et al., TAG 101:783, ‘00
BIN2 ant17
sld5
mo7a ABC171A Soule et al., J. hered. 91:483, ‘00
brh8
BIN3 xnt6
BIN4 btr1
btr2
lzd
BIN5 alm ABG471Kleinhofs BGN27:105, ‘96
abo9
sca
yst2
dsp10
BIN6 Rrs1 Graner et al., TAG 93: 421 ´96
Rrs.B87 BCD828 Williams et al., Plant Breed. 120:301, ‘01
Rh/Pt ABG396Smilde et al., 8th IBGS 2:178, ‘00
abo6
xnt3
msg5
ari-a
yst1
zeb1
ert-c
ert-ii
cer-zd
Ryd2 WG889B Collins et al., TAG 92:858, ‘96
BIN7 uzu
cer-r
BIN8 wst6
cer-zn
sld1
BIN9 wst1
BIN10vrs4
lnt1
gsh2
BIN11als
sdw1 PSR170 Laurie et al., Plant Breed. 111:198, ‘93
BIN12sdw2
BIN13Pub ABG389Kleinhofs et al., TAG 86:705, ‘93´
BIN14cur2
BIN15Rph10
fch2
BIN16eam10
Est1/2/3
rym4 MWG010 Graner & Bauer TAG 86:689, ‘93
rym5 MWG838 Graner et al., TAG 98:285, ‘99
Est4
ant28
Chr.4(4H)
BIN1 none
BIN2 fch9
sln
BIN3 int-c
Zeo3
Dwf2 Ole1 Ivandic et al., TAG 98:728, ‘99
Ynd
glo-a
rym1 ? Konishi et al., TAG 94:871, ‘97
BIN4 Kap X83518 Muller et al., Nature 374:727, ‘95
lbi2
zeb2
lgn3
BIN5 lgn4
lks5
eam9
msg24
BIN6 glf1
rym11 MWG2134 Bauer et al., TAG 95:1263, ‘97
Mlg MWG032 Kurth et al., TAG 102:53, ‘01
cer-zg
brh2
BIN7 glf3
frp
min1
blx4
sid
blx3
BIN8 blx1
BIN9 ert1
BIN10mlo P93766 Bueschges et al., Cell 88:695, ‘97
BIN11none
BIN12Hsh HVM067 Costa et al., TAG 103:415, ‘01
Hln
sgh1
yhd1
BIN13Bmy1 pcbC51 Kleinhofs et al., TAG 86:705, ‘93
rym8 MWG2307 Bauer et al., TAG 95:1263, ‘97
rym9 MWG517 Bauer et al., TAG 95:1263, ‘97
Wsp3
Chr. 5(1H)
BIN1 Rph4
Mlra
Cer-yy
Sex76 Hor2 Netsvetaev BGN27:51, ‘97
Hor5 Hor5 Kleinhofs et al., TAG 86:705, ‘93
BIN2 Hor2 Hor2 Kleinhofs et al., TAG 86:705, ‘93
Rrs14 Hor2 Garvin et al., Plant Breed. 119:193-196, ‘00
Mla6 AJ302292 Halterman et al., Plt J. 25:335, ‘01
BIN3 Hor1 Hor1 Kleinhofs et al., TAG 86:705, ‘93
Rps4
Mlk
BIN4 Lys4
BIN5, 6, 7. Mlnn; msg31; sls; msg4; fch3;
BIN6 amo1
BIN7 clh
vrs3
BIN8 fst2
cer-zi
cer-e
ert-b
MlGa
msg1
xnt7
BIN9 nec1
BIN10abo1
Glb1
BIN11wst5
cud2
BIN12rlv
lel1
BIN13Blp ABC261 Costa et al., TAG 103:415, ‘01
BIN14fch7
trd
eam8
Chr. 6(6H)
BIN1 Nar1 X57845 Kleinhofs et al., TAG 86:705, ‘93
abo15
BIN2 nar8 ABG378B Kleinhofs BGN27:105, ‘96
nec3
Rrs13
BIN3 none
BIN4 msg36
BIN5 nec2
ant21
msg6
eam7
BIN6 rob HVM031 Costa et al., TAG 103:415, ’01
sex1
gsh4
ant13
cul2 Crg4(KFP128) Babb & Muehlbauer BGN31:28, ‘01
fch11
mtt5
abo14
BIN7 none
BIN8 none
BIN9 Amy1 JR115 Kleinhofs et al., TAG 86:705, ‘93
Nar7 X60173 Warner et al., Genome 38:743, ‘95
Nir pCIB808 Kleinhofs et al., TAG 86:705, ‘93
mul2
cur3
BIN10lax-b
raw5
cur1
BIN11none
BIN12xnt5
Aat2
BIN13Rph11 Acp3 Feuerstein et al., Plant breed. 104:318, ‘90`
lax-c
BIN14dsp9
Chr. 7(5H)
BIN1 abo12
msg16
ddt
BIN2 dex1
msg19
nld
fch6
glo-b
BIN3 cud1 ABG705A
lys3
fst1
blf1
vrs2
BIN4 cer-zj
cer-zp
msg18
wst2
Rph2 ITS1 Borovkova et al., Genome 40:326, ’97
lax-a PSR118 Laurie et al., TAG 93:81, ’96
com1
ari-e
ert-g
ert-n
BIN5 rym3 MWG028 Saeki et al., TAG 99:727, ‘99
BIN6 none
BIN7 none
BIN8 none
BIN9 srhksuA1B Kleinhofs et al., TAG 86:705, ‘93
cer-I
mtt2
lys1
cer-t
dsk
var1
cer-w
Eam5
BIN10raw1
msg7
BIN11 Rph9/12 ABG712
Sgh2
lbi1
Rha4
raw2
BIN12none
BIN13rpg4 ARD5303 Druka et al., Mol.Gen.Genet. 264:283-290, ‘00
RpgQ ARD5304 Sun et al., Phytopath. 86:1299-1302, ‘96
BIN14var3
References:
Babb, S.L. and G.J. Muehlbauer. 2001. Map location of the Barley Tillering Mutant uniculm2 (cul2) on Chromosom 6H. BGN31:28.
Bauer, E., J. Weyen, A. Schiemann, A. Graner and F. Ordon. 1997. Molecular mapping of novel resistance genes against Barley Mild Mosaic Virus (BaMMV). Theor. Appl. Genet. 95:1263-1269.
Borovkova, I.G., Y. Jin, B.J. Steffenson, A. Kilian, T.K. Blake and A. Kleinhofs. 1997. Identification and mapping of a leaf rust resistance gene in barley line Q21861. Genome 40:236-241.
Börner, A., V. Korzun, S. Malyshev, V. Ivandic and A. Graner. 1999. Molecular mapping of two dwarfing genes differing in their GA response on chromosome 2H of barley. Theor. Appl. Genet. 99:670-675.
Brueggeman, R., N.Rostoks, D. Kudrna, A. Kilian, F. Han, J. Chen, A. Druka, B. Steffenson and A. Kleinhofs. 2002. The barley stem-rust resistance gene Rpg1 is a novel disease-resistance gene with homology to receptor kinases. Proc. Natl. Acad. Sci. USA 99:9328-9333.
Brunner, S., B. Keller and C. Feuillet. 2000. Molecular mapping of the Rph7.g leaf rust resistance gene in barley (Hordeum vulgare L.). Theor. Appl. Genet. 101:763-788.
Büschges, R., K. Hollricher, R. Panstruga, G. Simons, M. Wolter, A. Frijters, R. van Daelen, T. van der Lee, P. Diergaarde, J. Groenendijk, S. Töpsch, P. Vos, F. Salamini and P. Schulze-Lefert. 1997. The barley mlo gene: A novel control element of plant pathogen resistance. Cell 88:695-705.
Bulgarelli, D., N.C.Collins, G. Tacconi, E. Dellaglio, R. Brueggeman, A. Kleinhofs, A.M. Stanca and G. Vale. 2004. High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley. Theor. Appl. Genet. 108: 1401-1408.
Collins, N.C., N.G. Paltridge, C.M. Ford and R.H. Symons. 1996. The Yd2 gene for barley yellow dwarf virus resistance maps close to the centromere on the long arm of barley chromosome 3. Theor. Appl. Genet. 92:858-864.
Costa, J.M., A. Corey, M. Hayes, C. Jobet, A. Kleinhofs, A. Kopisch-Obusch, S.F. Kramer, D. Kudrna, M. Li, O. Piera-Lizaragu, K. Sato, P. Szues, T. Toojinda, M.I. Vales and R.I. Wolfe. 2001. Molecular mapping of the Oregon Wolfe Barleys: a phenotypically polymorphic doubled-haploid population. Theor. Appl. Genet. 103:415-424.
Drescher, A., V. Ivandic, U. Walther and A. Graner. 2000. High-resolution mapping of the Rph16 locus in barley. p. 95-97. In: S. Logue (ed.) Barley Genetics VIII. Volume II. Proc. Eigth Int. Barley Genet. Symp. Adelaide. Dept. Plant Science, Waite Campus, Adelaide University, Glen Osmond, South Australia.
Druka, A., D. Kudrna, F. Han, A. Kilian, B. Steffenson, D. Frisch, J. Tomkins, R. Wing and A. Kleinhofs. 2000. Physical mapping of the barley stem rust resistance gene rpg4. Mol. Gen. Genet. 264:283-290.
Edwards, M.C. and B.J. Steffenson. 1996. Genetics and mapping of barley stripe mosaic virus resistance in barley. Phytopath. 86;184-187.
Feuerstein, U., A.H.D. Brown and J.J. Burdon. 1990. Linkage of rust resistance genes from wild barley (Hordeum spontaneum) with isoenzyme markers. Plant. Breed. 104:318-324.
Freialdenhoven, A., B. Scherag, K. Hollrichter, D.B. Collinge, H. Thordal-Christensen and P. Schulze-Lefert. 1994. Nar-1 and Nar-2, two loci required for Mla12-specified race-specific resistance to powdery mildew in barley. Plant Cell 6:983-994.
Garvin, D.F., A.H.D. Brown, H. Raman and B.J. Read. 2000. Genetic mapping of the barley Rrs14 scald resistance gene with RLFP, isozyme and seed storage protein marker. Plant Breeding 119:193-196.
Giese, H., A.G. Holm-Jensen, H.P. Jensen and J.Jensen. 1993. Localisation of the Laevigatum powdery mildew resistance gene to barley chromosome 2 by the use of RLFP markers. Theor. Appl. Genet. 85:897-900.
Graner, A. and E. Bauer. 1993. RLFP mapping of the ym4 virus resistance gene in barley. Theor. Appl. Genet. 86:689-693.
Graner, A. and A. Tekauz. 1996. RFLP mapping in barley of a dominant gene conferring to scald (Rynchosporium secalis). Theor. Appl. Genet. 93:421-425.
Graner, A., S. Streng, A. Kellermann, A. Schiemann, E. Bauer, R. Waugh, B. Pello and F. Ordon. 1999. Molecular mapping and genetic fine structure of the rym5 locus encoding resistance to different strains of the Barley Yellow Mosaic Virus Complex. Theor. Appl. Genet. 98:285-290.
Haltermann, D., F. Zhou, F. Wei, R.P. Wise and P. Schulze-Lefert. 2001. The MLA6 coiled coil, NBS-LRR protein confers AvrMla6-dependent resistance specificity to Blumeria graminis f. sp. hordei in barley and wheat. Plt. J. 25:335-348.
Hansson, A., C.G. Kannangara, D. von Wettstein and M. Hansson. 1999. Molecular basis for semidomiance of missense mutations in the XANTHA-H (42-kDa) subunit of magnesium chelatase. Proc. Natl. Acad. Sci. USA 96:1744-1749.
Heun, M., A.E. Kennedy, J.A. Anderson, N.L.V. Lapitan, M.E. Sorrells and S.D. Tanksley. 1991. Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare). Genome 34:437-447.
Ivandic, V., S. Malyshev, V. Korzun, A. Graner and A. Börner. 1999. Comparative mapping of a gibberellic acid-insensitive dwarfing gene (Dwf2) on chromosome 4HS in barley. Theor. Appl. Genet. 98:728-731.
Johnson and A. Kleinhofs. 2004. unpublished.
Kleinhofs, A., A.kilian, M.A.Saghai Marrof, R.M. Biyashev, P. Hayes, F.Q. Chen, N. Lapitan, A..Fenwick, T.K. Blake, V. Kanazin, E. Ananiev, L.Dahleen, D. Kudrna, J. Bollinger, S.J. Knapp, B. Liu, M. Sorells, M. Heun, J.D. Franckowiak, D. Hoffman, R. Skadsen and B.J. Steffenson. 1993. A molecular, isozyme and morphologicsl map of the barley (Hordeum vulgare) genome. Theor. Appl. Genet. 86:705-712.
Kleinhofs, A. 1996. Integrating Barley RFLP and Classical Marker Maps. Coordinator’s report. BGN27:105-112.
Kleinhofs,.A. 2002. Integrating Molecular and Morphological/Physiological Marker Maps. Coordinator’s Report. BGN32:152-159.
Komatsuda, T., W. Li, F. Takaiwa and S. Oka. 1999. High resolution map around the vrs1 locus controlling two- and six-rowed spike in barley. (Hordeum vulgare). Genome 42:248-253.
Konishi, T., T. Ban, Y. Iida and R. Yoshimi. 1997. Genetic analysis of disease resistance to all strains of BaYMV in a Chinese barley landrace, Mokusekko 3. Theor. Appl. Genet. 94:871-877.
Kretschmer, J.M., K.J. Chalmers, S. Manning, A. Karakousis, A.R. Barr, A.K.M.R. Islam, S.J. Logue, Y.W. Choe, S.J. Barker, R.C.M. Lance and P. Langridge. 1997. RFLP mapping of the Ha2 cereal cyst nematode resistance in barley.Theor. Appl. Genet. 94:1060-1064.
Kurth, J., R. Kolsch, V. Simons and P. Schulze-Lefert. 2001. A high-resolution genetic map and a diagnostic RFLP marker for the Mlg resistance locus to powdery mildew in barley. Theor. Appl. Genet. 102:53-60.
Larson, S.R., K.A. Young, A. Cook, T.K. Blake and V. Raboy. 1998. Linkage mapping of two mutations that reduce phytic acid content of barley grain. Theor. Appl. Genet. 97:141-146.
Laurie, D.A., N. Pratchett, C.Romero, E.Simpson and J.W. Snape. 1993. Assignment of the denso dwarfing gene to the long arm of chromosome 3 (3H) of barley by use of RFLP markers. Plant. Breed. 111:198-203.
Laurie, D.A., N. Pratchett, J.H. Bezant and J.W. Snape. 1994. Genetic analysis of a photoperiod response gene on the short arm of chromosome 2 (2H) of Hordeum vulgare (barley). Heredity 72:619-627.
Laurie, D.A., N. Pratchett, R.A. Allen and S.S. Hantke. 1996. RFLP mapping of the barley homeotic mutant lax-a. Theor. Appl. Genet. 93:81-85.
Li. M., D. Kudrna and A. Kleinhofs. 2000. Fine mapping of a Semi-dwarf gene Brachytic1 in barley. p. 72-74. In: S. Logue (ed.) Barley Genetics VIII. Volume III. Proc. Eigth Int. Barley Genet. Symp. Adelaide. Dept. Plant Science, Waite Campus, Adelaide University, Glen Osmond, South Australia.
Müller, K.J., N. Romano, O. Gerstner, F. Gracia-Maroto, C. Pozzi, F. Salamini and W. Rhode. 1995. The barley Hooded mutation caused by a duplication in a homeobox gene intron. Nature 374:727-730.
Netsvetaev, V.P. 1997. High lysine mutant of winter barley - L76. BGN27:51-54.
Pratchett, N. and D.A. Laurie. 1994. Genetic map location of the barley developmental mutant liguleless in relation to RFLP markers. Hereditas 120:35-39.
Saeki, K., C. Miyazaki, N. Hirota, A. Saito, K. Ito and T. Konishi. 1999. RFLP mapping of BaYMV resistance gene rym3 in barley (Hordeum vulgare). Theor. Appl. Genet. 99:727-732.
Schmierer, D., A.Druka, D.Kudrna and A.Kleinhofs. 2001. Fine Mapping of the fch12 chlorina seedlig mutant. BGN31:12-13.
Schweizer, G.F., M. Baumer, G. Daniel, H. Rugel and M.S. Röder. 1995. RFLP markers linked to scald (Rhynchosporium secalis) resistance gene Rh2 in barley. Theor. Appl. Genet. 90:920-922.
Smilde, W.D., A. Tekauz and A. Graner. 2000. Development of a high resolution map for the Rh and Pt resistance on barley Chromosome 3H. p. 178-180. In: S. Logue (ed.) Barley Genetics VIII. Volume II. Proc. Eigth Int. Barley Genet. Symp. Adelaide. Dept. Plant Science, Waite Campus, Adelaide University, Glen Osmond, South Australia.
Soule, J.D., D.A. Kudrna and A. Kleinhofs. 2000. Isolation, mapping, and characterization of two barley multiovary mutants. J. Heredity 91:483-487.
Sun, Y., B.J. Steffenson and Y. Jin. 1996. Genetics of resistance to Puccinia graminis f. sp. secalis in barley line Q21861. Phytopathology 86:1299-1302.
Tohno-oka, T., M. Ishit, R. Kanatani, H. Takahashi and K. Takeda. 2000. Genetic Analysis of photoperiotic response of barley in different daylength conditions. p.239-241. In: S. Logue (ed.) Barley Genetics VIII. Volume III. Proc. Eigth Int. Barley Genet. Symp. Adelaide. Dept. Plant Science, Waite Campus, Adelaide University, Glen Osmond, South Australia.
Toojinda, T., L.H. Broers, X.M. Chen, P.M. Hayes, A. Kleinhofs, J. Korte, D. Kudrna, H. Leung, R.F. Line, W. Powell, L. Ramsey, H. Vivar and R. Waugh. 2000. Mapping quantitative and qualitative disease resistance genes in a doubled haploid population of barley (Hordeum vulgare). Theor. Appl. Genet. 101:580-589.
Warner, R.L., D.A. Kudrna and A. Kleinhofs. 1995. Association of the NAD(P)H-bispecific nitrate reductase structural gene with the Nar7 locus in barley. Genome 38:743-746.
Williams, K.J., A. Lichon, P. Gianquitto, J.M. Kretschmer, A. Karakousis, S. Manning, P. Langridge and H. Wallwork. 1999. Identification and mapping of a gene conferring resistance to the spot form of net blotch (Pyrenophora teres f. maculata) in barley. Theor. Appl. Genet. 99: 323-327.
Williams, K., P. Bogacki, L. Scott, A. Karakousis and H. Wallwork. 2001. Mapping of a gene for leaf scald resistance in barley line ’B87/14’ and validation of microsatellite and RFLP markers for marker-assisted selection. Plant Breed. 120:301-304.
Coordinator’s report: Barley Genetic Stock Collection
USDA-ARS, National Small Grains
Germplasm Research Facility,
Aberdeen, Idaho 83210, USA
Over 100 barley genetic male sterile stocks were planted in the greenhouse for seed increase. One hundred seventy-eight barley translocation stocks were increased in the field. In collaboration with Dr. Jerry Franckowiak, over 780 barley genetic stocks derived from crossing with cultivar ‘Bowman’ were planted in the field at Aberdeen in two-rowed single plot for seed increase and for agronomic evaluation.
Over one hundred-thirty barley genetic stocks were shipped to researchers in 2003.
Thirty-eight nitrate reductase deficient mutants obtained from Dr. Andy Kleinhofs (Table 1) were increased in the field in 2003.
Eleven globosum mutants from Dr. G. Fischbeck: glo-b.1 (W1), glo-b.2 (W2), glo-b.3 (W3), glo-b.4 (W4), glo-b.5 (W5), glo-d.1 (W6), glo-d.2 (W7), glo-d.3 (W8), glo-c (W9), glo-a (W10), and glo-e (W11) were increased in the field in 2003.
Ten translocation lines from Dr. Andreas Houben: T1-al, T1-6ai, T1-7ao, T2-5ah, T2-6aq, T2-7aj, T3-4ae, T3-7ax, T3-7aaa, and T5-6 ap, are being increased in the greenhouse, 2004.
Table 1. Nitrate reductase deficient mutants received from Dr. Andy Kleinhofs.
|
Entry |
Gene Symbol |
Line No. |
Function or Chemical Pathway |
|
|
|
nar1a |
AZ12 |
84-151 |
NADH NR structural gene |
|
2 |
nar1b |
AZ13 |
Spillman 6758 |
NADH NR structural gene |
|
3 |
nar1c |
AZ23 |
84-155 |
NADH NR structural gene |
|
4 |
nar1d |
AZ28 |
85-101 |
NADH NR structural gene |
|
5 |
nar1e |
AZ29 |
77-5-2 |
NADH NR structural gene |
|
6 |
nar1f |
AZ30 |
85-102 |
NADH NR structural gene |
|
7 |
nar1g |
AZ31 |
85-103 |
NADH NR structural gene |
|
8 |
nar1h |
AZ32 |
85-104 |
NADH NR structural gene |
|
9 |
nar1i |
AZ33 |
85-105 |
NADH NR structural gene |
|
10 |
nar1j |
Xno29 |
G84-1020-1034 |
NADH NR structural gene |
|
11 |
nar1k |
EMS29 |
86-117 (1-4) |
NADH NR structural gene |
|
12 |
nar1l |
EMS31 |
86-128-6 |
NADH NR structural gene |
|
13 |
nar1m |
AZ56 |
87-160-4 |
NADH NR structural gene |
|
14 |
nar1n |
AZ57 |
87-161-14 |
NADH NR structural gene |
|
15 |
nar1p |
AZ63 |
87-162-11 |
NADH NR structural gene |
|
16 |
nar1q |
AZ64 |
87-163-4 |
NADH NR structural gene |
|
17 |
nar1r |
AZ65 |
89-144-5 |
NADH NR structural gene |
|
18 |
nar1t |
AZ67 |
88-324-3 |
NADH NR structural gene |
|
19 |
nar1ab |
AZ76 |
87-165-3 |
NADH NR structural gene |
|
20 |
nar1ac |
AZ77 |
87-166-6 |
NADH NR structural gene |
|
21 |
nar1ai |
AZ79 |
87-167-3 |
NADH NR structural gene |
|
22 |
nar1aj |
AZ80 |
87-168-7 |
NADH NR structural gene |
|
23 |
nar1ao |
BSMV1 |
88-272-1 |
NADH NR structural gene |
|
24 |
nar2a |
AZ34 |
85-106 |
molybdenum cofactor |
|
25 |
nar2ad |
R9401 |
91-393-2 (het) |
molybdenum cofactor |
|
26 |
nar2ag |
R9201 |
92-195-2 (het) |
molybdenum cofactor |
|
27 |
nar3a |
Xno18 |
G84-996-1004 (het) |
molybdenum cofactor |
|
28 |
nar3b |
Xno19 |
G84-1005-1019(het) |
molybdenum cofactor |
|
29 |
nar3x |
AZ71 |
92-29-2 |
molybdenum cofactor |
|
30 |
nar4y |
AZ72 |
90-43-6 (het) |
molybdenum cofactor |
|
31 |
nar5o |
AZ62 |
88-59-5 |
molybdenum cofactor |
|
32 |
nar5s |
AZ66 |
93-161-2 |
molybdenum cofactor |
|
33 |
nar5u |
AZ68 |
91-378-4 |
molybdenum cofactor |
|
34 |
nar6v |
AZ69 |
90-59-8 (het) |
molybdenum cofactor |
|
35 |
nar7w |
AZ70 |
86-230 |
NAD(P)H NR structural gene |
|
36 |
nar8z |
AZ73 |
90-86-10 (het) |
molybdenum cofactor |
|
37 |
nar9ap |
AZ94 |
90-113-6 (het) |
nitrate toxicity |
|
38 |
- |
AZ86 |
90-92-2 (het) |
nitrate toxicity |
Coordinator’s report: Trisomic and aneuploid stocks
A. Hang
USDA-ARS, National Small Grains
Germplasm Research Facility,
Aberdeen, Idaho 83210, USA
There is no new information about trisomic and aneuploid stocks. A list on these stocks are available in BGN 25:104. Seed request for these stocks should be sent to the coordinator.
Coordinator’s report: Autotetraploids
Wolfgang Friedt
Institute of Crop Science and Plant Breeding I.
Justus-Liebig-University, Heinrich-Buff-Ring 26-32
e-mail: wolfgang.friedt@agrar.uni-giessen.de
The collection of barley autotetraploids (exclusively spring types) described in former issues of BGN is maintained at the Giessen Field Experiment Station of our institute. The set of stocks, i.e. autotetraploids (4n) and corresponding diploid (2n) progenitors (if available) have last been grown in the field for seed multiplication in summer 2000. Limited seed samples of the stocks are available for distribution.
Coordinator’s report: The Genetic Male
Sterile Barley Collection
M.C. Therrien
Agriculture and Agri-Food Canada
Box 1000A, RR#3, Brandon, MB
Canada R7A 5Y3
E-mail: MTherrien@agr.gc.ca
The GMSBC has been at Brandon since 1992. If there are any new sources of male-sterile genes that you are aware of, please advice me, as this would be a good time to add any new source to the collection. For a list of the entries in the collection, simply E-mail me at the above adress. I can send the file (14Mb) in Excel format. We continue to store the collection at -20oC and will have small (5 g) samples available for the asking. Since I have not received any reports or requests the last years, there is absolutely no summary in my report.
Coordinator’s report: Translocations and balanced tertiary trisomics
06466 Gatersleben, Germany
email: houben@ipk-gatersleben.de
Different translocation lines have been used to evaluate the influence of recombinantly-elongated chromosome arms on nuclear divisions in barley. Hudokova et al., 2002 confirmed a rule according to which half the length of the average spindle axis defines the upper tolerance limit for chromosome arm length. A slightly longer chromosome arm caused incomplete separation of sister chromatids in similar to70% of mitotic telophase cells and >2.5% of daughter cells showing a micronucleus, due to disruption of non-separated sister chromatids by the newly forming cell wall. In homozygous condition, this elongated chromosome mediated a slower growth and reduced fertility of the carrier plants. Its meiotic transmission was not impaired because of the larger spindle dimensions in meiocytes as compared to those in mitotic cells.
PCR with the DNA of translocation chromosomes and marker-specific primers has been used to merge genetically mapped microsatellite (MS) markers into the physically integrated restriction fragment length polymorphism (RFLP) map of barley chromosome 3H. It was shown that the pronounced clustering of MS markers around the centromeric region within the genetic map of this chromosome results from suppressed recombination. This yielded a refinement of the physically integrated RFLP map of chromosome 3H by subdivision of translocation breakpoints (TBs) that were previously not separated by markers. The physical distribution of MS markers within most of the subchromosomal regions corresponded well with that of the RFLP markers, indicating that both types of markers are similarly valuable for a wide range of applications in barley genetics (Künzel and Waugh, 2002).
Ten barley translocation stocks have been sent to An Hang (USDA, Aberdeen, USA). There were no requests for samples of balanced tertiary trisomics stock collection.
The collection is being maintained in cold storage. To the best knowledge of the coordinator, there are no new publications dealing with balanced tertiary trisomics in barley. Limited seed samples are available any time, and requests can be made to the coordinator.
References:
Hudakova S, Kunzel G, Endo TR, Schubert I, 2002: Barley chromosome arms longer than half of the spindle axis interfere with nuclear divisions. Cytogenetic and Genome Research 98: 101-107.
Künzel G, Waugh R, 2002: Integration of microsatellite markers into the translocation-based physical RFLP map of barley chromosome 3H. Theor.Appl.Genet. 105:660-66
Coordinator’s report: Eceriferum Genes
Udda Lundqvist
SvalöfWeibul AB
e-mail: udda@ngb.se
No research work on gene localization has been reported on the collections of Eceriferum and Glossy genes since the latest reports in Barley Genetics Newsletter (BGN). All information and descriptions done in Barley Genetics Newsletter (BGN) Volume 26 are valid and still up-to-date. The databases of the Swedish collection has been updated during the last months and will soon be searchable within International European databases. As my possibilities in searching literature are very limited, I apologize if I am missing any important papers. Please send me notes of publications and reports to include in next year’s reports. Descriptions, images and graphic chromosome maps displays of the Eceriferum and Glossy genes are available in the AceDB database for Barley Genes and Barley Genetic Stocks, and they get currently updated. Its address is found by: www.untamo.net/bgs
Every research of interest in the field of Eceriferum genes, ‘Glossy sheath’ and ‘Glossy leaf’ genes can be reported to the coordinator as well. Seed requests regarding the Swedish mutants can be forwarded to the Nordic Gene Bank, nordgen@ngb.se, all others to the Small Grain Germplasm Research Facility (USDA-ARS), Aberdeen, ID 83210, USA, anhang@uidaho.edu or to the coordinator at any time.
Coordinator´s report: Nuclear genes affecting the chloroplast
Diter von Wettstein
Department of Crop and Soil Sciences,
Washington State University
Pullman WA 99164-6420, USA
E-mail: diter@wsu.edu
The stock list and genetic information presented in the Barley Genetics Newsletter 21: 102-108 is valid and up-to-date. The stocks have been transferred to the Nordic Gene Bank. Requests for stocks available for distribution are to be either sent to:
Dr. Mats Hansson
Department of Biochemistry
Center for Chemistry and Chemical Engineering
Lund University
P.O.Box 124
SE-221 00 Lund, SWEDEN
Phone: +46-46-222 0105
Fax: +46-46-222 4534
E-mail: Mats.Hansson@biokem.lu.se
or to
Nordic Gene Bank
P.O. Box 41
SE-230 53 Alnarp
Sweden
Phone: +46 40 536640
FAX: +46 40 536650
e-mail: nordgen@ngb.se
References:
A. Hansson, R.D. Willows, T.H. Roberts and M. Hansson 2002. Three semidominant barley mutants with single amino acid substitutions in the smallest magnesium chelatase sununit form defective AAA+ hexamers. Proc. Natl. Acad. Sci. USA 99: 13944-13949.
U. Olsson, N. Sirijovski and M. Hansson 2003. Characterization of eight barley xantha-f mutants, deficient in magnesium chelatase. In: U. Olsson: Ferrochelatase and Magnesium chelatase: Metal chelation studies with mutants. pp.146. Doctoral Dissertation, Department of Biochemistry, Lund University. (4: 1-13). ISBN 91-7422-031-4. E-mail: Mats.Hansson@biokem.lu.se
Coordinator’s report: Semidwarf genes
J.D. Franckowiak
Department of Plant Sciences
North Dakota State University
Fargo, ND 58105, U.S.A.
Zhang 2003 traced the pedigree history of more than 350 dwarf and semidwarf barley cultivars released in China since 1950. The results showed that 68.4% of the cultivars were derived from six semidwarf accessions. ‘Chibadamai’, ‘Xiaoshanlixiahuang’, and ‘Changzhouluodamai’ are landraces and are in the pedigrees of many semidwarf cultivars released between 1950 and 1980. They have the same temperature sensitive dwarfing gene, uzu (uzu dwarf), which is located in chromosome 3HL (Zhang 2000). The other three sources are in released cultivars since 1980. ‘Aiganqi’ contains the uzu dwarfing gene. The dwarfing genes in ‘Zhepi 1' and ‘Yanfu Aizao 3' were not identified. Zhepi 3 was selected from a cross to Zhaori 19 and released by the Zhejiang Academy of Agricultural Sciences in 1978. Yanfu Aizao 3, which was released in Jiangsu Province in 1980, is a gamma-ray induced mutant of the Japanese barley ‘Zaoshu 3'.
In another paper, Zhang and Zhang 2003 reported their results from inheritance and allelism tests of reduced plant height using 25 Chinese accessions. Twenty of the accessions showed monogenic recessive inheritance patterns and four had digenic recessive patterns. Eleven of the monogenic accessions and two of the digenic accessions contained alleles at the uzu locus. Accession ‘1974E’ had the uzu gene plus a dominant dwarfing gene. Two of monogenic accessions and one digenic accession had alleles to the mutant in India Dwarf. Based on the crosses the uzu stock and India Dwarf, eight potential new dwarfing genes were identified.
Zhang and Zhang 2003 demonstrated that the dwarfing gene in India Dwarf is not sdw1 (semidwarf 1). Studies on the dwarfing gene in India Dwarf are not complete. Thus, its relationship to the sld5.e (slender dwarf 5) gene, which was reported to have been derived from Indian Dwarf (CIho 13994) (Franckowiak 2002), is unknown. Because the sld5.e gene backcrossed into Bowman produces relatively weak plants that have little agronomic potential, different genes may be present in the dwarf accessions from India. Zhang (personal communications) reported that the dwarfing gene in Zhepi 1 is allelic to the one present in the Chinese India Dwarf accession.
The dwarfing gene in Zhepi 1 may be of agronomic interest because it does not seem to delay maturity like the sdw1 gene. The sdw1 gene delays maturity about three days in the Upper Midwest of the USA and has not been incorporated into cultivars recommended for malting and brewing (Hellewell et al., 2000). The alleles sdw1.a (Jotun) and sdw1.c (denso) at the sdw1 locus are used to reduce plant height in many semidwarf cultivars in North America and Europe, respectively (Hellewell et al., 2000).
References:
Franckowiak, J.D. 2002. BGS 144, slender dwarf 5, sld5. BGN 32:94.
Hellewell, K.B., D.C. Rasmusson, and M. Gallo-Meagher. 2000. Enhancing yield in semidwarf barley. Crop Sci. 40:352-358.
Zhang, J. 2000. Inheritance of agronomic traits from the Chinese dwarfing gene donors ‘Xiaoshan Lixiahuang’ and ‘Cangzhou Luodamai’. Plant Breed. 119:523-524.
Zhang, J. 2003. Inheritance of plant height and allelism tests of the dwarfing genes in Chinese barley. Plant Breed. 122:112-115.
Zhang, J., and W. Zhang. 2003. Tracing sources of dwarfing genes in barley breeding in China. Euphytica 131:285-293.
Coordinator’s report: Ear morphology genes
Udda Lundqvist
SvalöfWeibull AB
SE-268 81 Svalöv, Sweden
e-mail: udda@ngb.se
No new research on gene localization or descriptions on different morphological genes have been reported since the latest reports in Barley Genetics Newsletter (BGN). All descriptions made in the volumes 26, 28, 29 and 32 are still up-to-date and valid. The databases of the Swedish Ear morphology genes have been updated during the last months and will soon be searchable within International European databases. As my possibilities in searching literature are very limited, I apologize if I am missing any important papers. Please send me notes of publications or reports to include in next year’s reports. Descriptions, images and graphic chromosome maps displays of the Ear morphology genes are also available in the AceDB database for Barley Genes and Barley Genetic Stocks, and they get currently updated. Its address is found by : www.untamo.net/bgs
Every research of interest in the field of Ear morphology genes can be report32 are still up-to-date and valid. The databases of the Swedish Ear morphology genes have been updated during the last months and will soon be searchable within International European databases. As my possibilities in searching literature are very limited, I apologize if I am missing any important papers. Please send me notes of publications or reports to include in next year’s reports. Descriptions, images and graphic chromosome maps displays of the Ear morphology genes are also available in the AceDB database for Barley Genes and Barley Genetic Stocks, and they get currently updated. Its address is found by : www.untamo.net/bgs
Every research of interest in the field of Ear morphology genes can be reported to the coordinator as well. Seed requests regarding the Swedish mutants can be forwarded to the Nordic Gene Bank,nordgen@ngb.se, all others to the Small Grain Germplasm Research Facility (USDA-ARS), Aberdeen, ID 83210, USA, anhang@uidaho.eduor to the coordinator at any time.
Coordinator’s report: Early maturity genes
Udda Lundqvist
SvalöfWeibull AB
SE-268 81 Svalöv, Sweden
e:mail: udda@ngb.se
No new research work on gene localization has been reported on the Early maturity or Praematurum genes since the latest reports in Barley Genetic Newsletter (BGN). All information and descriptions made in Barley Genetics Newsletter (BGN) Volumes 26 and 32 are valid and up-to-date. The database of the Swedish Praematurum genes has been updated during the last months and will soon be searchable within International European databases. As my possibilities in searching literature are very limited, I apologize if I am missing any important papers. Please send me notes of publications or reports to include in next year’s reports. Descriptions, images and graphic chromosome maps displays of the Early maturity or Praematurum genes are available in the AceDB database for Barley Genes and Barley Genetic Stocks, and they get currently updated. Its address is found by: www.untamo.net/bgs
Every research of interest in the field of Early maturity or Preamaturum genes can be reported to the coordinator as well. Seed requests regarding the Swedish mutants can be forwarded to the Nordic Gene Bank, nordgen@ngb.se, all others to the Small Grain Germplasm Research Facility (USDA-ARS), Aberdeen, ID 83210, USA, anhang@uidaho.edu or to the coordinator at any time.
Coordinator’s report:Wheat-barley genetic stocks
A.K.M.R. Islam
Faculty of Agriculture & Wine
The University of Adelaide, Waite Campus,
Glen Osmond, S.A. 5064, Australia
The production of amphiploid of Hordeum marinum with both durum and common wheat has been reported earlier. The amphiploid with common wheat has been backcrossed onto the wheat parent to change the cytoplasmic background to wheat. It has recently been possible to select six different monosomic addition lines (1Hm, 2Hm, 4Hm, 5Hm, 7Hm and possibly 6Hm) from among the second backcross progeny (Islam and Colmer, unpublished).
Disease and pest resistance genes
Brian Steffenson
Department of Plant Pathology
University of Minnesota
495 Borlaug Hall
1991 Upper Buford Circle
St. Paul, MN 55108-6030, USA
(in preparation)
