II.44. Allelism tests of some chlorina mutant genes of barley.
T. Tsuchiya, Debra A. Jensen* and T. E. Haus. Department of Agronomy, Colorado State University, Fort Collins, Colorado 80521, U.S.A.
*Undergraduate student. Part of this experiment was conducted by Miss Debra A. Jensen as a part of her project for Special Studies in Genetics (AG 495C).
Allelic relationships have been studied for 13 chlorina mutants which are shown in Table 1.
Table 1. Chlorina mutants used in allelism tests.
The genes f, fc, f7, f8, and f9 have been found to be separate loci (2,3). The gene f4 was located on the long arm of chromosome 1 (1L), and f5 on 1S by telotrisomic analysis (1,4). The gene f6 was associated with chromosome 6 (Tuleen, personal communication). The results obtained from this experiment are shown in Table 2.
Table 2. Phenotype of F1 hybrids and the results of F2 tests.
From the results shown in Table 2, the following conclusions are derived:
1. f3 is not allelic to f4, f6, f7, f8,
and f9.
2. f4 is not allelic to f5 or f8.
3. Chlorina gene in a variety Ko-C is not allelic to f even
though their character expressions are similar.
4. Chlorina gene in Colsess VII is not allelic to fc
in Colsess V, and does not show maternal inheritance.
5. Chlorina gene in 28-3117 is allelic to the genes in 28-3117-2 and
the gene in Nepal chlorina.
6. The possible maternal inheritance of these last 3 genes has not
yet been determined.
References:
1. Fedak, G. 1969. Ph.D. Thesis, University of Manitoba, Mimeo.
2. Nilan, R. A. 1964. The cytology and genetics of barley. Washington State University Press. 278 p.
3. Robertson, D. W. 1971. Recent information of linkage and chromosome mapping. Barley Genetics II:220-242.
4. Tsuchiya, T. 1972. Cytogenetics of telotrisomics in barley. BGN 2:93-98.
(Supported partly by NSF Research Grant, GB30493 to T. Tsuchiya).
