II.18. Allelism testing of some chlorina mutants.
A. Kleinhofs and B. J. Millham. Department of Agronomy and Soils, Washington State University, Pullman, Washington, 99163, U.S.A.
The mutants BHM 93 and BHM 95 have been located on chromosome 4 and BHM 78 on chromosome 2 (Tuleen, 1971). The mutant dES 20 also appears to be located on chromosome 4 (Tuleen et al., unpublished).
BHM 78 was crossed with lg. The F1 seedlings were normal green, indicating non-allelism. BHM 93, BHM 95, and dES 20 were crossed with f9 and lg4. The results are summarized in Table 1.
BHM 95 is allelic to lg4. The possibility that all 6 chlorina seedlings were from selfed seed was eliminated by examining esterase patterns of the F1 seedlings and parents. dES 20 is not allelic to either of the two light green genes presently located on chromosome 4 and thus represents a new locus on this chromosome.
References:
Tuleen, N. A. 1971. Translocation-gene linkages from F2 seedlings in barley. Barley Genetics II:208-212. R. A. Nilan (Editor).
Tuleen, N. A., A. Kleinhofs, and B. J. Millham (unpublished).
