J.D. Franckowiak1 and L.S. Dahleen2
1Department of Plant Sciences,
North Dakota State University and
2 Northern Crops Sciences Laboratory, USDA-ARS
Fargo, ND 58105, USA
Additional markers have been added to the barley molecular marker map developed for Steptoe-Morex population by the North American Barley Genome Mapping Project (NABGMP) (Kleinhofs et al., 1993; 1994; Kleinhofs, 1996). Thus, data for the mapping subset of 15 lines selected (Mgonja et al., 1995) from the 150 doubled haploid lines in the Steptoe-Morex population can be updated.
The large number of markers studied in the Steptoe-Morex population permits localization of the exchange points or breakpoints in each of the 150 lines of the population. The number of breakpoints between two markers, DNA exchanges between the original Steptoe and Morex chromosomes, provides an estimate of their linkage distance. These breakpoints in a selected group of lines or subset can be used to divide each of the seven barley chromosomes into segments. Each segment, over the 15 lines in the subset, has a specific and unique pattern regarding the original donor parent of that segment. If A is used for segments from Steptoe and B for those from Morex, the unique pattern for each segment can be represented as a different, but specific, sequence of A and B letters (Table 1).
Lines for a Steptoe-Morex subset were selected and the first listing of chromosomal segments was published in 1995 (Mgonja et al., 1995). The A-B data were used to divide the barley genome into 124 unique segments. There were 15 to 23 segments associated with each of the seven barley chromosomes. In the current listing of segments using A-B sequences, the genome could be divided into 134 segments using additional data on the Steptoe-Morex population. The added segments are presented in bold print in Table 1. Also, corrections of several errors, which were printed in Table 1 of Mgonja et al. (1995) are in bold print. Names of individual chromosomes and order of presentation were changed based on the Triticeae scheme for identifying barley chromosomes (Linde-Laursen, 1997).
If Steptoe and Morex differed for an unmapped marker, the response data collected on the 15 lines in the subset could be used to associate the marker with one, and only one, of the 134 segments. This subset from the Steptoe-Morex population has been used successfully to associate 127 RAPD markers with specific segments of the barley genome (Dahleen et al., 1997).
Table 1. Configurations by origin of barley chromosome segments in a selected subset of lines from the Steptoe-Morex population of the doubled haploid lines.
Marker locus Doubled haploid line
SM number* 007 031 041 045 065 074 126 144 150 164 165 179 182 184 200
Consecutive number 7 24 29 33 47 55 86 102 107 120 121 133 136 138 150
Chromosome 1H+ SM
ABA305** A§ B A - B - A A B B A A - B -
Aga6 - - - B - - B A B B A A A B -
Act8A B B B A B A B A B B A A A B A
Hor1 B B B A B B B A B A A A A B A
BCD98 B B B A B B B A B A A B A B A
ABG053 - B B A B B B - B A B B - B -
Ica1 - B B A A B B A B A B B A B A
ABG500A B A A A A B B A B A B B A B B
ABC160 B A A A A B B A A A B B A A B
Phy1 B A A A A B B A A A B A A A B
ABG464 B A A B A B B A A A B A A A A
BCD330 - A A B A - B B A A B A B A -
ABC307A B B A B B B B B A A B A B A A
cMWG733 B B A A B B B B A A B A B A A
AtpbA A B A A B B B B A A B A B A A
ABC322B A B A A B B A B A A B A B A A
ABC261 A B A A B B A B A A A A B A A
Aga7 A B A A A B A B A A A A B A -
ABG387A A B A B A B A - A A A A B A A
Chromosome 2H SM
ABG313A A B B B B A B B - A A B A A A
cMWG682 A B B B B A B B B A A B B A A
ABG58 A B B B B A A B B A A B B A A
ABG703B A B B B B B A B B A A B B A A
RbcS A B B B B B A B B A B B B A A
ABC156A A B B B B B A B A A B B B A A
ABG002 B B A B B B A B - A B B B A A
ABG358 B B A B A B A B A A B B B A A
Pox B B A A A B A B A A B B B A A
B15C A B A A - B A A A A - - B A A
CDO537 A A A A A B A A A A B B B A A
WG996 A A A A A B A A A A B B B A B
ABC451 A A A A A B A A A A B B A A B
ABC152D A B A A A B B A A A B B A A B
MWG503 B B A A A B B A A B B A A A B
BG072 B B B A A B B A A B B A A A B
ABC252 B B A B A B B A A B B A A A B
ABC157 A B A B A B B A A B B A A A B
ABC165 A B A B A B A A A B B A A B B
Pcr1 A A A B A B A A A B B A A B B
cMWG720 A A A B A B A A B B B A A B A
BG123A A A A B A B A A B B B A B B -
ABA005 A A A B A A A A B B B A B B A
Chromosome 3H SM
ABG316A B A B B B B A B B B B A B B A
ABC171 B A B B B B A A B B B A B B A
ABG460 B B B B B B A A - B B A B B A
ABG471 - B B B B B A A B B B A B B B
ABG398 B B B A B A A A B B B A B B B
BCD828 B B B B B A A A B B B A B B B
PSR156 B B B B B A A A B B B B B B B
MWG555B B B B B B B A A B B B B B B B
ABG453 B B B B A B A A B B B B B B B
ABR320 B B B B A B A A B A B B B B -
ABG499 B B A - - B A A A A B B B B B
His4B - - - - A B A - - A B - B A B
ABG004 A A A B A B A B A A B B A A B
ABC174 A A A B B B A B A A B B A A B
ABG495B A A A B - B A B - A A B A A B
ABC172 A A A A B B A B A A A B A A B
Chromosome 4H SM
WG622 A B B A B A A A A B B A B A A
MWG077 A A A B B A A A A B B A B A A
CDO669 A A A B B A A A A B B A B B -
BCD402B - - A B B A A A A B A A B B -
MWG635A A A A B B B A A A B A A B B A
Dhn6 A A A B B B A A A B A A B B B
Adh4 B A A B B B A A A B A A B B B
MWG058 B A A B B B B A A B A A B B B
WG464 B A A B B B B A A B A A B A B
bBE54A A A A B B B B A A B A A B A A
ABG472 A A A B A B B B A B A A B A A
KFP221 B A B B A B A B A B A A B A A
iAco2 B A B B A B A B A A A A B A A
ABG366 B A B B A B A B A A A A A A -
ABG319C B B B B A B A B A A A B B A A
Bmy1 B B B B A B B B A A A B B B -
Chromosome 5H SM
ABC483 B B B B - A A A B B A A A B A
ABG316B B B B B A A A A B B A A B B A
Phy3 B - - - A - - - - B A A A A B
ABG705 B B B B A A A B B A A A A A B
CDO669B - - - - - A B B B A A - A A B
Dor5 B B B B A A B B B A B A A A B
Ubi2 B B B B A A B B A A B A A A B
Ltp1 A B B B A A B B A A B A A A B
WG530 A A B B A A B B A A B A A A B
WG889 A A A B A A B B A A B A A A B
CDO348B A B A B A A B B A A B A A A B
ABC324 A B A B A A B B A A A A A A B
ABC302A A B A B A A A B A A A A B A B
CDO57B A B A A A A B B B A A A B A A
mSrh A B A A A A B B B A A A A A A
MWG514B A B A B B B B B B A B B A B A
ABG712 A B A B A B B A B A B B A B A
WG908 A B A B B B B A B A B B A B A
Cab1 A B B B B B B A B A B B B B -
ABC482 A B B B B B B A B A B B B A A
ABG391 A B B B B B B B B A B B B A A
ABG463 B B B B B B A B B A B B B A A
ABC309 B B B B B A A B A A B B B A -
MWG851C B B B B B A A B A A B B A A A
ABA304 B B B B A A A B A A B B A A A
Chromosome 6H SM
PSR167A B A B A A A B - B A B A A B -
Nar1 B A B A A A B A A A B A B B A
cMWG652A B B B A A A A A A A B B B B A
DD1.1C B B B B A A A A A A B B B B A
PSR106A B B B B A A A A A A B B B B B
PSR167B B B A B A A A A A A A B B B -
WG223B B B A B A A A A A A A B B A B
ABG474 B B B B A A A A A A A B B A B
ABC175 B B B B A A A B A A A B B A B
ABC170B A B B B A A A B A A A B B A B
MWG684D A B B B A A A B A B A B B A B
Nar7 A B B B B - A B A B A B B A B
Amy1 A B B B B B A B A B A B B B -
MWG934 A B B B A B A B B B A B B B B
MWG684C A A B B A B A B B B A B B B B
ABC170A A A B A A B A B B B A B B B B
MWG798A A B A A A B A B B B A B B B A
Chromosome 7H SM
Plc A A A A B A A A B A B B B A B
ABG077 A A A A B A A A B A B A B A B
BCD129 A A A A B A A A B B B A B A B
ABG320 A A A A B A A A B B B A B B B
iEst5 A B - A B A A A B B B A B B B
ABC169A B B A A B A A A B B B A B B B
ABG380 B B B A B A B A B B B A B B B
ABC154A B B B B B B B A B A B A B B B
ABC465B B B B B B B B A B A A A B B A
MWG003 B A B B A B B A B A A A A B A
VAtp57A B A A B A B B A A A A A A B A
Ubi1 B A A B B B B A A A A A B A -
RisP103 B A A B B B B A A A B A B A A
ABC305 B A A B B B B A A A B B B A A
ABG461 B B A B B B B A A A B B B B A
WG420 B B A B B B B A A A B B B B B
Cat3 A B A B B B B A A B B B B B B
* The 150 doubled haploid lines used in the mapping population are identified by a noncontinuous series of numbered between SM001 and SM200. Here, we present a "consecutive number" for each line to indicate its position in the series of lines for the complete data set of the Steptoe-Morex population. For example, SM031 is the 24th line in the population and is in the 24th position in the data set posted on GrainGenes.
+ The chromosome designation based on the Triticeae scheme for identifying barley chromosomes.
** Markers and letters in bold print and underlined have been changed or added since publication of first subset AB table for the Steptoe-Morex population (BGN 24:14-23).
§ Steptoe has the A allele at each locus and Morex has the B allele; a dash indicates missing data.
References:
Dahleen, L.S., D.L. Hoffmanm, J. Dohrmann, R. Gruber, and J. Franckowiak. 1997. RAPD marker placement on the barley Steptoe-Morex linkage map. (submitted to Genome).
Kleinhofs, A. 1996. NABGMP mapping update. BGN 25:62-70.
Kleinhofs, A., A. Kilian, D. Kudrna and the North American Barley Genome Mapping Project. 1994. The NABGMP Steptoe x Morex mapping progress report. BGN 23:79-83.
Kleinhofs, A., A. Killian, M.A. Saghai Maroof, R.M. Biyashev, P. Hayes, F.Q. Chen, N. Lapitan, A. Fenwick, T.K. Blake, V. Kanazin, E. Ananiev, L. Dahleen, D. Kudrna, J. Bollinger, S.J. Knapp, B. Liu, M. Sorrells, M. Heun, J.D. Franckowiak, D. Hoffman, R. Skadsen, and B.J. Steffenson. 1993. A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. Theor. Appl. Genet. 86:705-712.
Linde-Laursen, I. 1997. Recommendations for the designation of the barley chromosomes and their arms. BGN 26:1-3.
Mgonja, M.A., L.S. Dahleen, and J.D. Franckowiak. 1995. Subsets from mapping populations for localization of new genes in barley. BGN 24:14-23.