Additional information on the primary trisomic and telotrisomic analysis
of the chlorina mutant B2-4 or f13 in barley.
A. Hang and K. Satterfield
USDA-ARS, University of Idaho at Aberdeen, Idaho, USA.
E-Mail A03AHANG@attmail.com

Based on the results of a previous study; the chlorina mutant B2-4 or f13 was tentatively associated with chromosome 2 (Hang, 1992). However, when this mutant was crossed to telotrisomic 2S and to telotrisomic 2L for chromosome arm localization, many recessive mutants were found in the trisomic portion (Table 1). This indicated that this gene is not located on the short or the long arm of chromosome 2. In a recent study of a F2 population from crosses between f13 and primary trisomic for chromosome 2 (triplo 2), three recessive mutants were also found in the trisomic portion (Table 1). Therefore, it is unlikely this gene is located on chromosome 2 as previously reported.

Table 1. F2 segregation from crosses between f13 and Triplo 2L, Triplo 2S, and Triplo 2.

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                     2x             2x + 1            Total 
Cross           ______________   ______________   ______________               
Combination      +  f13  Total    +  f13  Total   +  f13  Total 
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Triplo 2L/f13   67   27   94     44   21   65    111  48   159 
Triplo 2S/f13   71   18   89     35   13   48    106  31   137 
Triplo 2/f13    63   22   88     24    3   27     90  25   115 
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Reference :

Hang, A. 1992. Trisomic analysis of the chlorina mutant B2-4, BGN 22:23-24.