This is an attempt to update the monoclonal antibody situation in barley since the last report (BGN 17:117-19). It appears there has been relatively little monoclonal antibody production activities in barley per se and it has been concentrated in a few laboratories. Table I lists monoclonal antibodies raised to barley antigens reported in the literature since 1987.
Monoclonal antibodies have been useful to investigate homology among plant components from different species, and therefore, phylogenetic relationships, through cross-reactivity experiments. For example, monoclonal antibodies to hordeins of cultivated barley (Hordeum vulgare L.) have been used to study hordein variation in the genus Hordeum (Pelger and von Bothmer, 1992). Monoclonal antibodies have been raised to components of cereal species of other genera and tested for cross-reactivity to barley as well. Table 2 lists such cases as reported in the relatively recent literature.
References:
Brett, G.M., E.N.C. Mills, S. Parmar, A.S. Tatham, P.R. Shewry, and M.R.A. Morgan. 1990. Monoclonal antibodies that recognize the repeat motif of the S-poor prolamins. J. Cereal Sci. 12:245-255.
Brown, P.H., R.P. Mecham, and T.-H. David Ho. 1988. Hormonal regulation of barley nuclease: investigations using a monoclonal antibody. Plant, Cell and Environ. 11:747-753.
Dawood, M.R., N.K. Howes, and W. Bushuk. 1989. Preparation of monoclonal antibodies against specific glaidin proteins and preliminary investigation of their ability to discriminate cereal cultivars. J. Cereal Sci. 10:105-112.
Høj, P.B., N.J. Hoogenraad, D.J. Hartman, H. Yannakena, and G.G. Fincher. 1990. Identification of individual (1-3, 1-4)-beta-D-Glucanase isoenzymes in extracts of erminated barley using specific monoclonal antibodies. J. Cereal Sci. 11:261268.
Masojc, P., J. Zawistowski, U. Zawistowska, and N.K. Howes. 1993. A combined monoclonal and polyclonal antibody sandwich ELISA for quantification of the endogenous alpha-amylase inhibitor in barley and wheat. J. Cereal Sci. 17:115124.
Pelger, S. and R. von Bothmer. 1992. Hordein variation in the genus Hordeum as recognized by monoclonal antibodies. Genome 35:200-207.
Skerritt, J.H. 1988. Hydrolysis of barley endosperm storage proteins during malting. I. Analysis using monoclonal antibodies. J. Cereal Sci. 7:251-263.
Skerritt, J.H. and A.S. Hill. 1990. Monoclonal antibody sandwich enzyme immunoassays for determination of gluten in foods. J. Agric. Food Chem. 38:1771-1778.
Skerritt, J. H. and P.Y. Lew. 1990. Homologies between grain storage proteins of different cereal species. 1. Monoclonal antibody reaction with total protein extracts. J. Cereal Sci., 11:103-121.
Søgaard, M., F.L. Olsen, and B. Svensson. 1991. C-terminal processing of barley aamylase I in malt, aleurone protoplasts, and yeast. Proc. Nat'l. Acad. Sci. USA 88:8140-8144.
Warner, R. L. 1994. Personal Communication. Washington State University, Pullman, U. S. A.
Whitelam, G.C., M.L. Anderson, E.E. Billett, and H. Smith. 1985. Immunoblot analysis of cross-reactivity of monoclonal antibodies against oat phytochrome with phytochrome from several plant species. Photochern. and Photobiol. 42:793-796.
Zawistowski, J., M. Ansell, U. Zawistowska, and N.K. Howes. 1992. A monoclonal antibody to endenous alpha-amylase inhibitor of barley. J. Cereal Sci. 15:1-4.
Zawistowski, J. and N.K. Howes. 1990. Production of monclonal antibodies against high M