Chromosomal position of the superoxide dismutase locus, Sod1 (=Sod B), in barley

V. P. Netsvetaev¹ and I. S. Krestinkov²
Plant Breeding and Genetics Institute¹
Odessa, 270036 and Technological Institute of Food Industry²
Sverdlova St., 112, Odessa, 270039, Ukraine

Superoxide dismutase of ripening seeds was separated in PAGE by the method of Beachamp and Fridovich (1971). Analysis of a spring barley collection showed polymorphism for five zymogramme areas designated as F (Fast), Ma (Middle a), Mb, Mc, and Md (Krestinkov et al., 1987). Isozymes in SOD F, SOD Mb, and SOD Md regions were inherited independently (Netsvetaev and Krestinkov, 1989).

SOD Mb was controlled by locus Sod1 (= Sod B). Linkage values of Sod1 with other genetic markers were calculated from data from a T1-5a X MK 6827 cross. The reciprocal translocation T1-5a (var. Gull) involving chromosome 1 and 5 had a normal type of isozyme, SOD Mbl, and MK 6827 had the exotic (much more slower) SOD Mb2. MK 6827 has gene sex6 (shrunken endosperm xenia 6) orientated proximally in chromosome 1 as proposed by Netsvetaev (1990, 1992). The data from the cross are shown in Table 1.

The locus Sod1 was found to be linked with the breakpoint in T1-Sa and the gene sex6. All other marker loci in both short and long arms of chromosome 5 were inherited independently of Sod1. The gene sex6 located proximally in chromosome l has been linked only with the locus Hrd A.

All the p-values of this cross indicate that Sod1 is orientated distally in barley's chromosome 1.

Table 1. Results from cross of T1-5a × MK6827 involving Sod1 and genetic factors in chromosome 1 and 5

References:

Beachamp, C. O., and I. Fridovich. 1971. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analyt. Biochem. 44:276.

Krestinkov, I. S., S. V. Biryukov, V. P. Netsvetaev, A. A. Linchevsky. 1987. Polymorphism of spring barley for superoxide dismutase isozymes of seed. (In Russian). Nauchno-Tekhnichesky Bulleten' VSGI, Odessa. No. 1(63):22-25.

Netsvetaev, V. P. 1990. Location of a shrunken endosperm gene, sex6, in barley. (In Russian). Nauchno-Tekhnichesky Bulleten' VSGI, Odessa. No. 1(75)31-35.

Netsvetaev, V. P. 1992. Use of double ditelosomics for gene location in barley. (In Russian). Cytology and Genetics (Kiev) 26:26-30.

Netsvetaev, V. P., and I. S. Krestinkov. 1989. Isoenzyme composition of grain superoxide dismutase and productivity of spring barley plants. (In Russian). Cytology and Genetics (Kiev). 23:33-37.

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