Telotrisomic analysis of a chlorina mutant gene (f3) in barley

S. Wang
Department of Agronomy, Colorado State University
Fort Collins CO 80523, USA

The mutant stock, BGS220 (T18-16), is a chlorina mutant. The gene was first described by Immer and Henderson (1943). The gene is simply recessive showing light yellow green color, and viability in homozygous condition (f3 f3). This gene has been tested and showed no allelic relationship with other mapped chlorina or light green mutant genes (fc, f, f2, f4, f5, f6, f7, f8, f9, and Ig5) (Tsuchiya, 1974; Tsuchiya et al., 1973). Previous studies by Shahla and Tsuchiya (1980) determined that this gene was located at chromosome 5, but the arm association has not been confirmed.

Telotrisomic analysis with Triplo SL was conducted by Shahla and Tsuchiya (1985) (Table 1). The results indicated that the f3 gene was not associated with the long arm of chromosome 5 even though the number of F₂ plants tested were small. Telotrisomic analysis with Triplo 5S confirmed that the gene is located on the short arrn of chromosome 5 (Table 1). With 126 F₂ plants tested in the disomic portion and total population, the ratio of wild type vs f3 type deviated from 3:1. There was no homozygous mutant observed in trisomic portion of the 34 triplo 5S plants studied.

This result adds a useful marker gene f3 to the short arm of chromosome 5. The f3 gene is the first conventional marker gene confirmed to be on the short arm of chromosome 5. Most genes mapped on this arm are mildew resistance and hordein genes.

Acknowledgements:

I wish to thank Dr. T. Tsuchiya for his support and assistance with this study and for providing the genetic stocks.

References:

Immer, F. R., and M. T. Henderson. 1963. Linkage studies in barley. Genetics 28:419-440.

Shahla, A., and T. Tsuchiya. 1980. Trisomic analysis of the gene (f3) for chlorina 3 in barley. J. Heredity 71:359-361.

Shahla, A., and T. Tsuchiya. 1985. Further information on telotrisomic analysis in barley. BGN 15:26-27.

Tsuchiya, T. 1974. Further results of allelism testing in barley. BGN 4:82-85.

Tsuchiya, T., D. A. Jensen, and T. E. Haus. 1973. Allelism test of some chlorina mutant genes of barley. BGN 3:71-72.

Table 1. F₂ data from telotrisomic analyses of crosses to BGS220, which contained the f3 gene for mutant gene chlorina 3.

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