Location on barley chromosome 1 of loci Estl and Est2, coding esterases 1 and 2

V. P. Netsvetaev
Biochemical Genetics Laboratory, Plant Breeding and Genetics Institute
Odessa-36, 270036, Ukraine

The leaf esterases were analysed by using polyacrylamide gel electrophoresis, pH 8.3 (Netsvetaev, 1992a,b). Esterases 1, 2, 4, and 5 were identified by means tester set of varieties (Table 1) received from H. B. Johansen in 1989. The data of addition wheat-barley lines (obtained from A. K. M. Islam in 1988) analyses confirmed that Estl, Est2, and Est4 are located on barley chromosome 3 and EstS on chromosome 1.

Differences in esterase isozymes composition between varieties having the fastest and slowest electrophoretic mobility were controlled by new loci designated as Estl l and Estl2, respectively.

Recombination value frequencies between genes Estl, Est2, f2 (chlorina 2), al (albino lemma), cu2 (curly 2), and uz (semi-brachytic) were calculated on results of the cross cu × al+f2. P1 (=cu) had alleles F2, Al, cu2, uz, Est1Af, est2 (absent esterase 2 activity, and P2 (=al+f2) had alleles f2, al, Cu2, Uz, Est1Ca, Est2. The data obtained is presented in Table 2.

The arrangement of these six genes on chromosome 3 are as follows

al-uz-cu2-f2-Est2

Est1

and are shown in Figure 1.

Loci Estl - Est2 are tightly linked and no recombinants in this region were found.

The information reported here is additional to data previously obtained by Nielsen and Hejgaard (1985), Konishi and Matsuura (1989), and Netsvetaev (1992b) concerning Est location on chromosome 3.

Table 1. Tester set of varieties using for identification esterase loci in barley (Designation according to Hvid and Nielsen, 1977; Nielsen and Johansen, 1986; Linde-Laursen et al., 1987; *Kahler and Allard, 1970; ** Netsvetaev, 1992b).

Table 2. Linkage values for cross cu × al+f2 involving six genes chromosome 3.

Figure 1.

References:

Hvid, S., and G. Nielsen. 1977. Esterase isoenzyme variants in barley. Hereditas 87: 155- 162.

Kahler, A. L., and R. W. Allard. 1970. Genetics of isozyme variants in barley I. Esterases. Crop Sci. 10:444-448.

Konishi, T., and S. Matsuura. 1989. Chromosomal localization of two esterase isozyme loci on chromosome 3 in barley. BGN 19:46-48.

Linde-Laursen, I., G. Nielsen, and H. B. Johansen. 1987. Distribution of enzyme markers at 37 loci in a pedigree of European spring barley. Hereditas 106: 241-251.

Netsvetaev, V. P. 1992a. Use of double ditelosomics for gene location barley. Cytology and Genetics (Kiev). 1:26-30.

Netsvetaev, V. P. 1992b. Identification of the barley leaf esterase 11, 12 and their genetic control. Genetika control. Genetika (USSR). 27: 105-119.

Nielsen, G., and J. Heigaard. 1985. Linkage of a tripsin inhibitor locus (Itc1) and two esterase loci (Esll and Est4) with marker loci on barley chromosome 3. BGN 15:16-18.

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