The mutant stock, CSU B8-9 (zb2, previously assigned as zb_c), is a zebra striped leaf mutant in CSU barley genetic stock collection. The gene zb2 is a simple recessive and has been studied by Tsuchiya (1974), Tsuchiya and Haines (1975), Seip and Tsuchiya (1978), and Furst (1983). They genetically analyzed this mutant with all trisomic stocks except Triplo 2. Primary trisomic analysis using Triplo 2 was conducted in 19891990. The mutant for zb2 (CSU B8-9) was used as pollen parent and crossed with Triplo 2. F[2] segregation results and results from others were shown in Table 1. The result from Table 1 indicated that zb2 gene is not associated with chromosome 2. Seven out of twenty trisomic F₂ plants were mutants, and the total ratio was very close to 3:1. Data from Table 1 also suggested that zb2 could not be associated with chromosomes 3, 4, 5, 6, and 7. The only suspicious one is chromosome 1, since only 17 F₂ plants have been studied. Further investigation is now underway.

References:

Furst, E. C. 1983. Primary trisomic analysis of three mutant genes in barley. M.S. Thesis, Colorado State University.

Seip, L., and T. Tsuchiya. 1978. Trisomic analysis of lg4 and zb_c. BGN 8:86-89.

Tsuchiya, T. 1974. Report for genetic stock center in Fort Collins, Colorado. BGN 4:107-110.

Tsuchiya, T., and R. L. Haines. 1975. Trisomic analysis of nine mutant genes in barley. BGN 5:67-69.