The male sterile stock number, heading date, gene symbol, segregation ratios, selfing behavior and anther morphology of 124 new mutants are shown in Table 1. These mutants originated as follows: msg,,jv and msg,,jw, spontaneous mutants collected by J.D. Franckowiak from drillstrips at Fargo, ND; msg37jx (a semidwarf), locus symbol assignment and origin is described by Franckowiak and Hockett (1988); msg,,jy, spontaneous mutant from yield plots and msg,,jz and msg,,ka, spontaneous mutants from breeders seed field found by E.A. Hockett; msg,,kb through msg,,oo are spontaneous mutants collected in Wales in 1977 through 1982 by C.A. Foster (1986). The stocks sent by Foster numbered 154 but only 118 are assigned symbols in Table 1. Many of these stocks were lost due to poor seed viability. This resulted in failure to segregate for male sterility because of low plant numbers in the Bozeman plantings.
Almost all the mutants behaved as single recessive genes for male sterility segregating 3:1 for fertile:sterile plants when a heterozygous plant was selfed (Table 1). More male sterile plants than are expected were found for msg37jx, but the number of plants is low and this stock segregated in a 3:1 ratio at Fargo (Franckowiak, 1987). The stocks msg,,ma and msg,,oc also had more male sterile plants than expected but Foster (1986) indicated they segregate 3 fertile : I sterile plant in Wales. However, the segregation of msg,,oc is almost a 1:1 ratio at Bozeman indicating possible gamete lethality or inviability of one of the two fertile genotypes. The mutants with fewer male sterile plants than expected were msg,,kk, msg,,ko, msg,,kq, msg,,le, msg,,ln, msg,,ly, msg,,mp, msg,,ms, msg,,na, msg,,nn, and msg,,oo. All these stocks segregated in a 3:1 ratio in Wales according to Foster (1986), and only the probability for the ratio for msg,,ln, msg,,na, and msg,,oo was less than 1%.
In general the anther size note taken at Bozeman and at Wales are in agreement (Table 1). Several mutants classified as rudimentary anther size at at least one of the locations (nsg,,lu, msg,,mm msg,,of) had some selfed seed. Further investigation of the behavior of these stocks shows segregation for partially sterile plants as well as plants with 0% selfing. The classification for anther size must have been made on the 0% selfing plants. One other stock, msg,,lw, shows 1% selfing even though the anthers were classified as rudimentary in Wales (Foster, 1986).
References:
Foster, C.A. 1986. (personal communication)
Franckowiak, J.D. 1987. (personal connunication)
Franckowiak, J.D. and E.A. Hockett. 1988. Identification of three new loci which control male sterility of barley. Barley Genet. Newsl. 18:
