II.3. Induced multinoded mutants in barley.
G. Prasad, and D.K. Tripathi, Department of Agricultural Botany, S.D.J. Post-Graduate College, Chandesar, Azamgarh 276128 (U.P.), India. "R"
During the study of chemical mutagenesis in a 6-rowed barley cultivar DL 36, two multinoded mutants were isolated from the M2 generation. One mutant 'multinoded branched' (mnb) (CMS 10) was induced by 0.3% diethylsulphate in 12 hours water presoaked seeds, the frequency being 0.123% among the viable mutants. Plant height of this mutant was 66.2 cm which was 81% of the mother cultivar. It produced a large number of tillers and the main shoot and some tillers were branched (Fig. 1). Number of internodes in this mutant was 16, where as in DL 36, there were 5 to 6 internodes. The other mutant 'multinoded' dwarf (mnd) (CMS 75) was induced by 10-3M sodium azide in 12 hours water presoaked seeds. Its frequency was 0.209% among the viable mutants. It was dwarf, the plant height being 69% of DL 36 and produced 10 to 11 internodes. Significant differences were observed between the mother cultivar and the mutants regarding many characters (Table 1). Both of the mutants were late in flowering. Such mutants may be utilized for fodder purpose as suggested by Ram (1982).
F1 phenotype of the crosses between the multinoded mutants and the cultivar DL 36 was normal. In F2, segregation occurred in 3 normal:1 mutant ratio (Table 2) which indicated that they were governed by single recessive genes.
Table 2. Inheritance of induced multinoded mutants.
Figure 1. Multinoded branched mutant (some tillers and leaves removed).
Reference:
Ram, M. 1982. Gene revolution in barley. All India Coordinated Barley
Impr. Project (ICAR). IARI Regional Stn., Karnal, Ed. Km. Neelam Rani,
pp. 96.
