IV. l9. Coordinator's Report. The genetic male sterile barley collection.
E. A. Hockett, USDA-ARS, Plant and Soil Science Dept., Montana State University, Bozeman, Montana 59717. U. S. A.
One new numbered locus ha6 been assigned to the genetic male sterile series (Table 1). These results of crossing a homozygous male sterile plant with a heterozygous male sterile plant show that the spontaneous mutant msg34av is not allelic with any of the previously numbered loci. The msg34av, male sterile stock number 314 (5) behaves as a single recessive gene and did not set any selfed seed when bagged at Bozeman (4). Although the 5:0 ratio testing msg34av and msg30c for allelism is low, these mutants are undoubtedly not allelic since msg30c has some selfing and female sterility at Bozeman while msg34av does not (4).
The heading date, segregation ratios, selfing behavior, and anther morphology of 149 new mutants are shown in Table 2. These mutants originated as follows: ms,,ec, spontaneous mutant collected by D. R. Biggerstaff and R. F. Eslick; msg2ed and ms,,ee, spontaneous mutant collected by R. T. Ramage (7); msg,,ef, spontaneous mutant collected by E. A. Hockett; msg,,eg, spontaneous mutant collected by H. Ahokas (1); msg,,eh through msg,,ix, spontaneous mutants collected by C. A. Foster and coworkers (2,3) (msg,,ew and msg,,ex derived through diethyl sulphate treatment); and msg,,iy through msg,,ju, spontaneous mutants collected by P. Hagberg in Sweden (6). Almost all the mutants behaved as single recessive genes for male sterility segregating 3:1 for fertile:sterile plants when a heterozygous plant was selfed (Table 2). The stock msg2ed had a shortage of male sterile plants in the Bozeman planting, but results previously published (7) indicates it behaves as a single recessive gene. The gene stocks from Wales which did not fit a 3:1 segregation ratio were as follows: too many sterile plants - msg,,ep, msg,,fp, msg5fr, and msg,,hk; too few sterile plants - msg,,fj, msg,,gq, msg,,hl, msg,,ic, msg,,ir, and msg,,ix. All these stocks may be single genes for male sterility and further data could confirm this, with the exception of msg,,ic which had 152 partially sterile plants: 2 sterile plants in the segregating rows. The mutants msg,,fp and msg5fr satisfactorily fit a 3:1 ratio in Wales (2) although the numbers given are smaller than those reported here. Five of the stocks from Sweden, ms,,jk through msg,,jn and msg,,jq had fewer male sterile plants than expected (Table 2). Although these stocks behaved as single recessive genes in Sweden (6) it may be that the seeds containing the msgmsg gene had reduced viability in Bozeman.
References:
1. Ahokas, H. 1978. Male sterile mutants of barley. VI. Tests of restoration
by the cytoplasm of Hordeum bulbosum, and reports of new mutants.
Barley Genet. Newsletter 8:12-15.
2. Dawi, D. A. and C. A. Foster. 1983. Allelism studies of new genetic male sterile barley stocks in the WPBS collection. Barley Genet. Newsletter 13:9-11.
3. Foster, C. A., M. Fothergill and A. D. Hale. 1983. The WPBS genetic male sterile barley collection. Barley Genet. Newsletter 13:6-8.
4. Hockett, E. A. 1972. Coordinator's report on the genetic male sterile barley collection. Barley Genet. Newsletter 2:139-144.
5. Hockett, E. A. and D. A. Reid. 1981. Spring and winter genetic male-sterile barley stocks. Crop Sci. 7.1:655-659.
6. Lehman, L. 1982. Unpublished data of Per Hagherg. Personal Communication.
7. Ramage, R. T. and J. E. Flora. 1981: Allele tests and chromosome location of two male sterile mutants in ingrid. Barley Genet. Newsletter 11:36-37.
