II. 21. Callus induction and plant generated from some diploid and tetraploid barley. (1)
A. Hang (2) and J.D. Franckowiak, Department of Agronomy, North Dakota State University, Fargo, North Dakota 58105 U.S.A.
(1) Supported in part by Competitive Research Grant No. 59-2381-0-1-460-0
from USDA/ARS. (2) Presently Research Associate, Department of Agronomy,
Colorado State University, Fort Collins, Colorado 80523.
Tissue culture of barley has been successfully reported by several workers (Cheng and Smith, 1975; Wilson et al., 1976; Dale and Deambrogio, 1979; Miller and Brinkman, 1980).
In this report, immature embryos 8 to 11 days after pollination of several diploid and tetraploid barleys have been used as explants for callus induction. B5 medium (Gamborg and Wetter, 1975) with 1 mg of 2,4-D was used. All the cultures were kept in the dark for 3 to 5 weeks for callus induction. For plant regeneration, calli were transferred to B5 with reduced concentration of 2,4-D to 0.5 mg. Results are shown in Table 1.
Table 1. Tissues initiated from young embryos of diploid and tetraploid barley.
Some genotypes have shown more potential for callus induction than others in the same medium. The response of different genotypes to media has been reported by Miller and Brinkman (1980).
Of 51 plants regenerated from the diploid barley, 50 plants had 2n = 14, one plant was trisomic with 2n = 15.
References:
Cheng, T.Y. and H.H. Smith. 1975. Organogenesis from callus culture of Hordeum vulgare. Planta 123:307-310.
Dale, P.J. and E. Deambrogio. 1979. A comparison of callus induction and plant regeneration from different explants of Hordeum vulgare. Z, pflanzenphysiol. 94:65-77.
Gamborg, O.L. and L.R. Wetter. 1975. Plant tissue culture methods. Published by the National Research Council of Canada. Prairie Regional Laboratory, Saskatoon, Saskatchewan, Canada.
Miller, J.P, and M.A. Brinkman. 1980. Progress in barley tissue and cell culture. Barley Newsl. 24:22.
Wilson, H.M., B. Boroughi-Wehr, G. Mix and H. Gaul. 1976. Callus cultures of Hordeum vulgare: initiation, growth, and organogenesis. Barley Genet. Newsl. 6:86-87.
