II. 7. Breakpoints localized to chromosome arm or region in 26 translocation lines of barley using Giemsa C-banding.
Ib Linde-Laursen, Agricultural Research Department, Riso National Laboratory, DK-4000 Roskilde, Denmark. "R"
Identification of barley chromosomes and chromosome arms by means of
Giemsa C-banding (Linde-Laursen 1975) renders it possible to localize
translocation breakpoints reliably to arm based either on changes in
banding patterns or chromosome morphology. The breakpoints have been localized
to chromosome arms or more narrow regions in 26 translocation lines (Table
1) comprising nine of the eleven lines of the translocation tester set
proposed by Persson (1969a). The chromosome morphology of the two remaining
lines, T1-5f and T6-7i, were not sufficiently changed to assign breakpoints
reliably to arm by the method used. The use of the C-banding technique
verified the position of questionable breakpoints or identified changes
of locations in fourteen lines, in which the previous information had been
obtained by other methods; however, some of the changes in designation
may derive from the changes of arm designations undertaken in the course
of time.
References:
Hagberg, A., L. Lehmann, and P. Hagberg. 1978. Segmental interchanges
in barley. II. Translocations involving chromosomes 6 and 7. Z. PflanzenzHchtg.
81:89-110.
Linde-Laursen, I. 1975. Giemsa C-banding of the chromosomes of 'Emir' barley. Hereditas 81:285-289.
Linde-Laursen, I. and 0. A. Olsen. 1976. Identification of an interchange between chromosome 2 and 5 in Riso mutant 56 - a high lysine line. BGN 6:43-44.
Persson, G. 1969a. An attempt to find suitable genetic markers for dense ear loci in barley I. Hereditas 62:25-96.
Persson, G. 1969b. An attempt to find suitable genetic markers for dense ear loci in barley II. Hereditas 63:1-28.
