II. 5. Additional information on the association of zbc2 for zoned leaf with chromosome 5 in barley. (1)
A. Shahla, A. and T. Tsuchiya, Department of Agronomy, Colorado State University, Fort Collins, Colorado 80523 U.S.A.
(1) Supported in part by USDA/SEA Competitive Research Grant 82-CRCR-1-1020 and CSU Hatch project.
The zoned leaf or Zebra striped leaf in the stock Utah T 41 (zbc2) (Tsuchiya, 1983) were associated with chromosome 5 by primary trisomic analysis (Furst and Tsuchiya, 1983). In order to confirm previous results on Triplo 5 - zbc2 relationship, the trisomic Fl plants between Triplo 5 and zbc2, were backcrossed with zbc2 homozygotes as the female. Since there is almost no pollen transmission in primary trisomics of barley, chromosome numbers in the progenies of trisomic backcrosses were counted. The segregation ratio is shown in Table 1.
Table 1. Segregation ratio in the backcross generation (BC1) between Triplo 5 and zbc2 homozygotes.
The result showed that segregation ratio was trisomic in the backcross generation, confirming the previous results by Furst and Tsuchiya (1983) that the incomplete dominant gene zbc2 is located on chromosome 5, although the zbc2 homozygote is less than expected.
References:
Furst, E. and T. Tsuchiya. 1983. Primary trisomic analysis of three
mutant genes in barley. BGN 13:44-46.
Tsuchiya, T. 1983. Proposed new gene symbol for two different mutant types for zebra Colorado or zoned leaf. BGN 13:84.
