Fig. 5. Mitochondrial dehydrins from the crowns of winter wheat grown in the field. Proteins were fractionated in 11 % SDS-PAAG (left, gel) and transferred to nitrocellulose membrane (right, blot), which was incubated in the solution of antibody against dehydrins (1:1,000). Immunoreactive groups were detected with secondary antibody conjugated with alkaline phosphatase. Time of sampling: (1) Sept.; (2) Oct.; (3) Nov.; (4) Dec.; (5) Feb.; (6) March; (7) Apr.; and (8) May. Molecular weights (kD) of dehydrins are indicated on the right.