Fig. 11. Association of dehydrins with membranes of winter wheat cells during cold treatment. Lanes 1 and 3, membrane dehydrins from control wheat seedlings; lanes 2 and 4, from cold hardened those. Membrane proteins of tonoplast (lanes 1 and 2) and plasma membrane (lanes 3 and 4) were extracted with sample buffer at 50-60°C for 20 min and separated in 12 % SDS-PAAG. Proteins were transferred to the nitrocellulose membrane and detected with antibody against dehydrins. Molecular weights of dehydrins are indicated on the right.