Fig. 10. Dehydrin localization in mitochondria of winter wheat seedlings. Mitochondria were isolated from control (1, 2) and acclimated (3, 4) to 4 C for 7 days wheat seedlings. Mitochondria treated with pronase E at 37 C for 1 h (2, 4). Dehydrins were revealed using antibody against dehydrins (1:1000) and secondary antibody conjugated with alkaline phosphatase. Molecular weights of dehydrins are indicated on the right.