ITEMS FROM SPAIN

 


UNIVERSIDAD POLITECNICA DE MADRID
Departamento de Biotecnologia, E.T.S. Ing. Agronomos.- C. Universitaria, 28040, Madrid, Spain.

A. Delibes, I. López-Braña, M. J. Montes, and C. González-Belinchón.

UNIVERSITY OF LLEIDA AND
INSTITUTE FOR FOOD AND AGRICULTURAL RESEARCH AND TECHNOLOGY (UdL-IRTA)

Center of R&D, Rovira Roure 177, 25198 Lleida, Spain.

J.A. Martín-Sánchez, E. Sin, C. Martínez, and A. Michelena.

JUNTA DE EXTREMADURA. SERVICIO DE INVESTIGACION AGRARIA.
Departamento de Fitopatología. Ap. 22 CP. 06080 Badajoz, Spain.

J. del Moral, F. Pérez-Rojas, F. J. Espinal, and M. Senero.

 

Studies in relation to the Hessian fly-resistance gene (H30) transferred from the wild grass Aegilops triuncialis to hexaploid wheat. [p. 142-144]

The transfer line TR-3531 (42 chromosomes), derived from the cross 'T. turgidum/Ae. triuncialis//T. aestivum' and carrying the H. avenae resistance gene Cre7 (Romero et al. 1998), showed a high level of resistance to the M. destructor biotype prevailing in southwestern Spain. A single, dominant gene (H30) determines the Hessian fly resistance in this introgression line (Delibes et al. 2001), and its linkage with an isozyme marker (Acph-U1) has been studied. A phosphatase marker, resolved into two components, is present in the TR-3531 line, Ae. triuncialis (UC), Ae. umbellulata (U), and the amphiploid Chinese Spring/Ae. umbellulata (ABDU), but is absent in Ae. caudata (C). Linkage between H30 and the Acph-U1 marker (associated with the U genome) was determined by analyzing 126 individual (TR-3531/H-10-15) F2 plants. The kernels were cut transversely and the halves without embryos were used to obtain phosphatase zymograms, an enzymatic system associated in wheat with homoeologous group 4 (Delibes et al. 1997a). The linkage in this cross is not very tight, which would be consistent with the recombination expected of the ability of the C genome to suppress the Ph-diploidization mechanism of wheat (Romero et al. 1998).

F2 progeny, derived from crosses between different wheat cultivars from Uniform Hessian Fly Nursery (UHFN and H-93-33 transfer line) and with other sources of resistance, and TR-3531 were tested for resistance in field conditions in order to determine if the new resistance gene was allelic with the H3, H5, H6, H12, H13, H18, H21, or H27 genes. Although the cultivars from UHFN are effective against Hessian fly in the United States, there is no evidence that the selected genes confer resistance to biotype present in Azuaga (southwestern Spain). The results and summarized in Table 1. All UHFN cultivars tested with different genes were resistant to this biotype, except the cultivar Abe with the gene H5, which showed a inconsistent reaction. The resistance gene H30 in line TR-3531 is nonallelic with respect to the genes H3, H6, H12, H13, H18, and H21 present in wheat cultivars from UHFN and H27 in the introgression line H-93-33 (Delibes et al. 1997a and b). Previously, we demonstrated that H30 in TR-3531 line was not allelic with respect H9 and H11 present in cultivars Ella and Kay, respectively (Delibes et al. 2001).

Table 1. Hessian fly reactions of the parents, F1, and F2 populations from crosses between wheats with different resistance genes and the resistant line TR-3531, a carrier of the H30 gene. Hessian fly reaction of line R-3531 is 25R:0S. R = resistance and S = susceptibility to Hessian fly. UHFN = Uniform Hessian Fly Nursery.
 Cultivar or UHFN line  Gene  Chromosome

 Hessian fly reaction

Crosses between cultivars of the UHFN / TR-3531

 UHFN line  No. F1 plants  No. F2 plants  X2(1:d.f.) 15:1 ratio
 R:S  R:S  R:S  Value  Probability (P)
 Howell  H3  5A  18:0  7:0  154:8  0.46  0.5
 Monon  H3  5A  19:0  9:0  187:6  3.25  0.05<P<0.1
 Caldwell  H6  5A  29:0  8:1  218:14  0.02  0.9
 841453 H15-1-1-2-5-2  H12  5A  26:1  2:0  95:1  4.45  0.01<P<0.05
 86925 RA1-16  H13  6DL  18:2  3:0  140:7  0.55  0.3<P<0.5
 Brule  H18  ---  15:4  7:0  141:2  5.74  0.01<P<0.05
 KS86HF012-23-6  H21  2BS  19:0  7:0  243:6  6.27  0.01<P<0.05
 H-93-33  H27  4M^v^  21:0  7:0  50:5  0.76  0.3<P<0.5

Advanced lines with the H30 gene were obtained by backcrossing the transfer line and different commercial wheats (cultivars Anza, Betres, Cajeme, Cartaya, Marius, Rinconada, and Osona) as recurrent parents. In all advanced lines, the infestation level was higher, but in the same range, than the donor. Several agronomic characteristics were studied in 16 advanced lines and the results of three of the lines are summarized in the Table 2. The best results were achieved with the Ma-6 line, which displayed good agronomic characteristics, in comparison to the susceptible controls, for the three traits studied. Another fact that increases the importance of this line is that it also carries the CCN resistance gene Cre7.

Table 2. Agronomic characteristics of three advanced lines with the H30 resistance gene in comparison to three bread wheat cultivars.
 Cultivar or line  Yield (g/m^2^)  Kernels/spikelet  1,000-kernel weight (g)
 T. aestivum cv. Osona  1,240.57  2.71  29.49
 T. aestivum cv. Astral  897.55  1.64  30.17
 T. aestivum cv. Adalid  1,687.51  2.65  33.14
 Ma6: TR/OS//OS/3/RN/4/OS/5/RN/6/AZ 3  1,914.12  3.00  43.37
 Ma4: TR/BT//AL/3/MA/4/3*BT 2  1,336.08  2.84  27.65
 Ma3: TR/3*OS//4*CYÄ/3/CJ 4  1,486.86  2.64  32.50
 Least significant difference (LSDP<0.05)  409.16  0.47  5.05
 Abbreviations used: Ma = advanced lines, TR = TR-353 line, AZ = Anza, BT = Betres, CJ = Cajeme, CY = Cartaya, MA = Marius, OS = Osona, RN = Rinconada, = selfing.

Cooperation with other institutions. We are cooperating with Acorex (Cooperative of Extremadura farmers).

Financial support. This work was supported by grants AGL2001-3824-CO4, PTR 95-0496-OP CO1021001 from CICYT "Comision Interministerial de Ciencia y Tecnologia" of Spain and IPR99A042 (Junta de Extremadura).

References.

Publications. [p. 143-144]