Fig. 5. Changes in aquaporin content in the membrane proteins of winter wheat plants after a 1-month deadaptation. The microsomal fraction was separated from leaves (1, 2) and crowns (3, 4). Plants were taken from field (1, 3) or left for 30 days under ambient conditions (2, 4). Proteins were fractionated on 12 % SDS­PAGE gels. Aquaporins were identified by specific antibodies against TIP (tonoplast-intrinsic protein, A) and PIP (plasmalemma-intrinsic protein, B). Arrows and numerals between the images indicate the molecular size markers.