Fig. 1. The effect of different respiratory chain inhibitors on state-4 oxygen uptake by winter wheat mitochondria isolated from control, stressed (short-term cold stress), and hardened (hardening) shoots. The inhibitors were added in the sequence shown reading left to right.

• A. 10 mM malate in the presence of 10 mM glutamate used as a substrate of oxidation (M+SE, n = 3-32).
• B. 8 mM succinate in the presence of 5 mM glutamate used as a substrate of oxidation (M+SE, n = 3-19).
• C. 1 mM NADH used as a substrate of oxidation (M+SE, n = 5-14).