Fig. 27. Western blotting with antibodies to dehydrins and RAB-proteins of winter wheat crowns heat-stable proteins. Proteins were extracted from freshly sampled crowns frozen in liquid nitrogen. Electrophoresis was in 10 % SDS-PAGE. Proteins were transferred to membranes and probed using anti-dehydrin and anti-RAB antibodies. The figure illustrates representative immunochemical membrane. Molecular weights of the RAB-proteins are indicated in the left and those of the dehydrins on the right.