OKLAHOMA

OKLAHOMA STATE UNIVERSITY

Plant Pathology Department, 110 Noble Research Center, Stillwater, OK 74078-9947, USA.

R.M. Hunger and J.L. Sherwood (Plant Pathology Department), and M.E. Payton (Department of Statistics).

Barley yellow dwarf virus.

BYDV occurs in Oklahoma every year and has been a serious problem during each of the past several years. During 1995-96, Gaucho 480F (Gustafson, Inc., Dallas, TX) seed treatment was evaluated for control of aphids and BYDV near Stillwater, OK. Plots consisted of three, 3-ft rows of each HRWW cultivar planted 0.50 to 0.75 in. deep on 6 October, 1995. The experiment was performed as a split-plot in a randomized complete block design with six replications. Insecticide seed treatment (Gaucho 480F @ 3 oz/cwt) was the main-plot treatment, and cultivar was the subplot treatment. Aphid populations were determined three times during the growing season by counting the number of aphids in 1 ft of row randomly designated in each plot. BYDV incidence (the percentage of flag leaves exhibiting BYDV symptoms) was assessed on 15 May, 1996. Presence of BYDV in foliage was confirmed by ELISA. Aphid counts were transformed with the square root transformation due to the count nature of the variable, and multiple comparisons (LSD a = 0.05) were made on the simple effects of insecticide and cultivar because of the presence of interaction between cultivar and insecticide treatment. BYDV incidence was transformed using the arcsine­square root transformation due to the percentage nature of the variable. No variety by insecticide interactions were detected for the variables BYDV incidence, height, 1,000-kernel weight or yield, so main effects of these factors were examined.

The 1995-96 season was conducive to aphids, and populations in fields surrounding the trial were extremely high. Aphid populations 70 days after planting (15 December, 1995) were significantly lower on plants from seed treated with Gaucho 480F. By 158 days after planting (12 March, 1996) and 168 days after planting (22 March, 1996), aphid populations remained significantly greater on plants from seed not treated with Gaucho. There was no difference between aphid incidence on the varieties when not treated with Gaucho (controls) except for Karl, which had significantly more aphids on all three dates. Cultivars treated with Gaucho had a lower BYDV incidence, were significantly taller, and yielded significantly more than untreated controls. There was no significant difference in 1,000-kernel weight between treated and control plots. The cultivars 2137, 2163, and Custer exhibited similar BYDV incidence, with 2137 having the greatest yield and Custer having the highest 1,000-kernel weight. This trial demonstrated that Gaucho 480F used as a seed treatment at 3 oz/cwt significantly reduced aphid populations and BYDV incidence on wheat. The cost of this treatment is approximately $20.00/bu of seed. In years when aphid pressure and subsequent BYDV incidence are low, use of Gaucho at the 3 oz rate probably would not provide a positive economic return on the investment.

Table 1. Effect of Gaucho 480F insecticide treatments on winter wheat and its effect on the control of aphid populations and the incidence of barley yellow dwarf virus.

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Aphids (no/ft of row)

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15 December, 1995. 12 March, 1996. 22 March, 1996.

_________________________ _________________________ ________________________

Gaucho Gaucho Gaucho

Cultivar Control 3 oz/cwt Control 3 oz/cwt Control 3 oz/cwt

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2137 43.2 a1 ***2 0.0 a 117.7 a *** 58.2 a 216.2 a *** 115.2 a

2163 44.3 a *** 0.0 a 125.5 a *** 64.2 ab 222.2 a *** 115.3 a

Custer 46.5 a *** 0.0 a 125.7 a *** 81.3 c 230.5 a *** 144.5 b

Jagger 46.3 a *** 0.0 a 123.8 a *** 71.8 bc 212.0 a *** 129.5 ab

Karl 71.3 b *** 0.3 a 162.5 b *** 72.2 bc 276.7 b *** 122.8 ab

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Plant 1,000-kernel

BYDV incidence height (cm) Yield (bu/acre) weight (gm)

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Gaucho 3 oz/cwt 23.1 a1 66.4 a 77.0 a 25.2 a

Control 50.8 b 59.1 b 64.4 b 24.8 a

Cultivar

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2137 27.5 a1 64.9 ab 81.7 a 25.2 b

2163 34.3 a 62.5 bc 70.0 b 22.6 c

Custer 29.7 a 59.8 c 71.0 b 28.3 a

Jagger 42.9 b 65.7 a 66.9 b 23.2 c

Karl 50.4 b 60.8 c 63.6 b 25.6 b

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1 Means followed by the same letter within a column are not significantly different (LSD, a = 0.05).

2 *** indicates significant difference between aphid counts within dates (LSD, a = 0.05).

Wheat leaf rust.

The incidence of all foliar wheat diseases including wheat leaf rust was nearly zero, because of extremely dry conditions for most of the spring in Oklahoma during 1995-96. No leaf rust ratings of breeder lines or cultivars were made in the field in 1996, because of the low incidence of leaf rust. Seedling reaction to wheat leaf rust of entries in regional breeder nurseries (1996 Southern Regional Performance Nursery, 1996 Northern Regional Performance Nursery, and 1996 Regional Germplasm Observation Nursery) were conducted and are available in the annual USDA publication that summarizes testing of regional nurseries (Dr. Jim Peterson, USDA-ARS, Lincoln, NE) or by contacting R.M. Hunger (e-mail: rmh@okway.okstate.edu) at Oklahoma State University.

Table 2. Visual assessment (VA), ELISA values, yield, and 1,000-kernel weight of entries from the

1996 Southern and Northern Regional Performance Nurseries evaluated for reaction to wheat soilborne

mosaic virus.

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VA 1 Yield 1,000-kernel

Entry and selection number 1-4 ELISA +/- sd 2 (gm) weight (gm) 3

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1996 Southern Regional Performance Nursery.

01 CI1442 ('Kharkof') 3.2 1.10 +/- 0.21 1.8 13.0*

02 CI13996 ('Scout 66') 3.5 1.11 +/- 0.22 5.1 13.7

03 PI495594 ('TAM-107') 3.3 1.09 +/- 0.18 10.2 18.3

04 KS93U206 1.8 1.17 +/- 0.12 24.8 21.0

05 OK91P648 1.0 0.72 +/- 0.62 44.4 21.7

06 OK93P735 1.0 1.07 +/- 0.13 36.2 20.0

07 OK93P634 1.6 1.08 +/- 0.09 28.3 23.7

08 OK92403 1.0 0.03 +/- 0.04 36.9 16.7

09 TX91D6913 1.0 0.01 +/- 0.03 67.8 24.3

10 TX91D6991 1.0 0.03 +/- 0.01 84.1 25.3

11 TX92V3108 3.7 1.02 +/- 0.20 19.1 19.3

12 HBI0531-A2 3.8 1.05 +/- 0.23 14.9 19.7

13 TX93V5919 1.0 0.47 +/- 0.57 30.1 19.0

14 TX93V5922 4.0 1.02 +/- 0.21 2.3 14.7

15 TX93V4927 4.0 1.02 +/- 0.27 1.7 11.3

16 TX92V2519 1.6 0.46 +/- 0.61 13.5 19.0

17 TXGH12588-105 3.7 1.10 +/- 0.16 17.3 21.3

18 CO910424 1.0 0.05 +/- 0.02 39.3 24.3

19 CO910748 4.0 1.02 +/- 0.23 2.6 12.5*

20 KS91H153-2 3.3 1.03 +/- 0.22 12.8 20.3

21 KS941064-3 1.0 0.79 +/- 0.50 50.2 21.7

22 KS941064-6 1.0 1.10 +/- 0.18 52.7 22.3

23 KS940935-7-2-1 1.0 0.05 +/- 0.04 69.0 23.7

24 KS940935-125-5-2 1.0 0.46 +/- 0.28 57.1 23.0

25 KS85W663-11-6-MB 1.0 1.10 +/- 0.15 41.0 22.0

26 KS84W963-9-39-3-MBX 1.0 0.06 +/- 0.06 46.1 17.7

27 NE90476 3.8 1.08 +/- 0.20 10.4 18.3

28 NE92458 1.2 0.76 +/- 0.63 40.9 21.7

29 NE92646 1.3 0.67 +/- 0.50 39.6 21.0

30 NE93405 3.2 0.98 +/- 0.03 13.8 23.0

31 NE93427 1.0 0.56 +/- 0.46 35.2 20.3

32 WX92-0408 1.0 0.37 +/- 0.50 21.2 17.7

33 W93-460 1.0 1.03 +/- 0.09 40.2 23.3

34 T702 1.0 0.76 +/- 0.58 46.8 21.3

35 T834 1.0 1.07 +/- 0.02 49.5 24.0

36 T812 3.0 1.04 +/- 0.04 22.3 19.3

37 T861 2.8 0.96 +/- 0.09 22.2 21.7

38 T89 2.0 1.02 +/- 0.08 33.3 22.3

39 G1878 1.0 0.05 +/- 0.05 46.4 20.0

LSD (P = 0.05) 19.1 4.1

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Table 2 (continued). Visual assessment (VA), ELISA values, yield, and 1,000-kernel weight of entries

from the 1996 Southern and Northern Regional Performance Nurseries evaluated for reaction to wheat

soilborne mosaic virus.

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VA 1 Yield 1,000-kernel

Entry and selection number 1-4 ELISA +/- sd 2 (gm) weight (gm) 3

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1996 Northern Regional Performance Nursery.

01 CI1442 ('Kharkof') 3.8 1.06 +/- 0.09 0.9 14.0*

02 CI17439 ('Roughrider') 3.0 1.06 +/- 0.05 0.7 15.0*

03 PI511307 ('Abilene') 1.3 0.54 +/- 0.65 23.7 19.7

04 SD89153 3.0 1.06 +/- 0.08 8.5 11.7

05 SD91192 3.2 1.18 +/- 0.08 4.9 14.7

06 SD92107 2.5 1.16 +/- 0.08 11.0 15.7

07 SD92124 1.2 0.50 +/- 0.61 40.7 20.3

08 SD92174 1.0 0.40 +/- 0.48 19.0 13.0

09 SD92191 3.2 1.11 +/- 0.09 3.1 16.0*

10 SD92227 3.3 1.06 +/- 0.09 7.4 18.0*

11 SD92266 3.7 1.09 +/- 0.11 9.9 14.3

12 ND9257 3.0 1.03 +/- 0.06 11.4 15.7

13 ND9272 4.0 1.10 +/- 0.06 0.8 -.*

14 ND9274 4.0 1.08 +/- 0.10 0.9 12.0*

15 NE92628 2.3 1.10 +/- 0.05 18.4 22.7

16 NE92662 1.0 0.07 +/- 0.09 24.1 21.3

17 NE92652 1.0 0.52 +/- 0.71 34.5 23.7

18 NE93554 3.3 1.31 +/- 0.42 8.4 23.7

19 NE93613 3.7 1.15 +/- 0.07 7.0 13.3

20 XNH1798 3.0 1.21 +/- 0.03 6.7 8.3*

21 XNH1800 3.2 1.17 +/- 0.02 2.5 15.7

22 MT88046 3.3 1.19 +/- 0.03 5.8 15.0

23 MTS92042 3.8 1.00 +/- 0.10 1.5 16.5*

24 ID0467 3.3 1.21 +/- 0.01 4.3 13.0*

25 ID0483 4.0 1.13 +/- 0.08 1.7 15.0*

26 AMP3JP4A7A 1.0 0.41 +/- 0.65 28.9 17.7

27 AMQ3KC4C7B 1.0 0.79 +/- 0.67 9.3 13.3

28 AMQ3NQ4A7D 3.2 1.16 +/- 0.06 4.3 14.5*

29 AMQ3KF4B7A 3.3 1.15 +/- 0.07 0.2 -.*

LSD (P = 0.05) 10.3 3.9

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1 VA = visual assessment index.

2 ELISA +/- sd = absorbance value from the enzyme-linked immunosorbent assay +/- standard deviation.

3 1,000-kernel weights followed by an asterisk (*) are the average of < 3 reps. The LSD should not be

used to compare these values.

Wheat soilborne mosaic virus.

The reaction to WSBMV was determined in the field for wheat entries in the 1996 Southern Regional Performance Nursery (SRPN) and the 1996 Northern Regional Performance Nursery (NRPN) (Table 2, p. 330-331). The trial was conducted near Stillwater, OK. The experimental design for each nursery was a randomized complete block with three replications. Plots consisted of three, 2-ft rows per entry. Seed was planted about 0.75 in. deep at a rate of 24 seeds/2-ft row on 9 October, 1996. Rows of the wheat cultivars Vona (WSBMV-susceptible, wheat spindle streak mosaic (WSSMV)-susceptible), Sierra (WSBMV-resistant, WSSMV-susceptible), and Hawk (WSBMV-resistant, WSSMV-resistant) were planted between reps to monitor the presence and distribution of WSBMV and WSSMV. Entries were assessed for stunting and mosaic symptoms on 5 March and 2 April, 1996, using a visual assessment (VA) index of 1-4, where 1 = no stunting, no mosaic, 2 = slight stunting and/or slight mosaic, 3 = moderate stunting and/or slight mosaic, and 4 = severe stunting and/or severe mosaic. Young foliage was collected from each row of each entry on 5 March, for evaluation by the ELISA (Hunger et al. 1991, Crop Sci 31:900-905), and the trial was harvested on 7 June (SRPN) and the 11 June (NRPN).

No foliar diseases occurred, but symptoms indicative of root rot were scattered throughout the trial. Wheat spindle streak mosaic virus was detected by ELISA (absorbance values > 0.80) in seven of 16 Vona and Sierra samples and may have contributed to stunting and mosaic symptoms. WSBMV was uniformly distributed throughout the trial area. Vona plants (n = 28) randomly selected from the trial had VA indices of 3 or 4 and values from ELISA for WSBMV from 0.73 to 1.47 (mean = 1.22). Sierra and Hawk plants (n = 15) randomly assessed from the trial had VA indices of 1 or 2, and values from ELISA for WSBMV from 0.01 to 0.18 (mean = 0.04) with two exceptions (0.97 and 1.13). Based on VA, 24 SRPN entries and seven NRPN entries were resistant to WSBMV (VA < 2.0). Most of these entries had ELISA values indicative of no detectable virus or virus concentrations less than those of the susceptible check cultivar Vona. Higher yield and greater 1,000-kernel weight also were associated with the resistant entries.

Wheat streak mosaic virus.

Upon completing the serological analysis of several isolates of WSMV (Montana et al. 1996), the coat protein region of many of these isolates has been cloned and sequenced, and the sequence analysis is being completed. In addition, the coat protein open reading frame is being placed in a vector for transformation of wheat in cooperation with Dr. Arron Guenzi, Department of Agronomy, Oklahoma State University.

Breeding for disease resistance.

Backcrossing of germplasm lines to adapted HRWW cultivars was continued. These germplasm lines were obtained from crossing emmers (T. dicoccoides) resistant to leaf rust, WSBMV, and/or powdery mildew with adapted HRWW cultivars. Other germplasm lines obtained from crossing Eastern European cultivars with HRWW cultivars were evaluated in the field. However, the low incidence of leaf rust in the field in 1996 resulted in evaluations of limited value. These lines are being evaluated again in the field during 1996-97.

Personnel.

After serving for nearly 11 years as the Department Head of Plant Pathology, Dr. Larry J. Littlefield has stepped down to a faculty position. Larry will be expanding his research on documenting the life cycle of Polymyxa graminis, and initiate other research involving electron microscopy. Dr. John L. Sherwood currently is serving as the interim head.

Publications.

Chenault K, Hunger R, and Sherwood J. 1996. Comparison of the nucleotide sequence of the coat protein open reading frame of nine isolates of wheat streak mosaic rymovirus. Virus Genes 13:187-188.

Evans CK, Hunger RM, and Siegerist WC. 1996. Inoculum density and infection efficiency of conidia and conidiophores of isolates of Pyrenophora tritici-repentis. Plant Dis 80:505-512.

Hunger RM, Siegerist WC, Smith EL, Morgan G, and Payton ME. 1996. Reaction of the Southern Regional Performance Nursery (SRPN) to wheat leaf rust, 1995. Biol Cul Tests for Control of Plant Dis 11:86.

Montana JR, Hunger RM, and Sherwood JL. 1996. Serological characterization of wheat streak mosaic virus isolates. Plant Dis 80:1239-1244.


PLANT SCIENCE RESEARCH LABORATORY, USDA-ARS

1301 N. Western St., Stillwater, OK 74075, USA.

C.A. Baker, J.D. Burd, N.C. Elliott, M.H. Greenstone, D.B. Hays, S.D. Kindler, D.W. Mornhinweg, D.R. Porter, K.A. Shufran, and J.A. Webster.

Host plant resistance/germplasm enhancement.

Topcrossing of RWA-resistant wheat lines to high-performance recurrent parents continued. Efforts are ongoing to develop RWA-resistant wheat lines for all market classes that are grown in areas where RWA is a problem. Over 55 advanced lines derived from eight different RWA resistance sources were planted for seed increase and evaluation in preparation for germplasm release; these lines include hard red and hard white winter, hard and soft white spring, and hard red spring wheats. Over 400 winter wheat lines derived from crosses with seven different sources of RWA resistance were evaluated in the field in Stillwater. Approximately one quarter of these lines performed well enough in this stressful environment to warrant further field evaluations. These field-selected lines plus additional greenhouse selections made up the approximately 500 lines being planted in the field in Stillwater this fall. Genetic diversity studies continued. Results of genetic studies of topcross populations helped explain most of the conflicting reports in the literature concerning inheritance of RWA resistance. The 1997 Regional Germplasm Observation Nursery was tested for greenbug and RWA resistance. Resistance mechanisms were characterized for greenbug multibiotype-resistant GRS-1201. Bioassay experiments were developed to identify bird cherry-oat aphid resistance in seedling wheat and barley.

Biological control.

Modeling. Progress towards development of a computer model for simulation of the landscape and regional population dynamics of the RWA during 1996 included: 1) landcover classification and ground-truthing of Landsat MSS imagery covering eastern Colorado, the Oklahoma Panhandle, the Texas Panhandle, and western Kansas; 2) field studies of RWA population dynamics in Canada wild rye were conducted in southeastern Colorado; 3) acquisition and processing of USGS digital elevation data, NRCS Statsgo soils data, and USGS digital line graphs for the entire region of the Great Plains in which RWA populations typically occur; and 4) acquisition and processing of long-term NOAA meteorological data for over 500 sites distributed throughout the above-mentioned portion of the Great Plains.

The influences of prey abundance, within-field vegetation, and composition and diversity of the surrounding landscape matrix on communities of aphidophagous insects were studied in wheat fields. Within field variables such as aphid abundance and crop density were important for explaining variation in the abundance of adult Coccinellidae in wheat fields. The density of herbaceous and grassy weeds in fields did not influence the abundance of any aphidophagous species. The proportion of cultivated land and diversity of the landscape mosaic in the section (1.5 km2) surrounding the sampled field influenced the abundance of some aphidophagous species, as did the proportion of cultivated land in the eight sections immediately adjacent to the section that included the sampled field. The influence of the mosaic extended beyond the eight surrounding sections and was detectable at a scale greater than 100 km2.

Evaluation of aphid natural enemy effectiveness. Initial production of monoclonal antibodies for serological gut analysis of aphid predators revealed a major cross-reacting protein in several cereal aphid species. New fusions are planned using greenbug and RWA homogenates from which the cross-reacting protein has been removed by precipitation. A postdoctoral fellow is being recruited to support the development of DNA hybridization probes to detect endoparasitoids within aphid hosts. A colony of Aphelinus albipodus was established to provide DNA for this effort, and colonies of A. asychis and A. varipes are being established.

A replicated study of spider species diversity and abundance in relation to cereal aphids was begun in autumn 1996, in Lamar, CO. Each replicate comprises two 1-ha wheat plots, the first planted to TAM 107 and the second to HALT. Aphids were counted by collecting tillers and multiplying the number per tiller by the number of tillers per square meter. Spider densities were obtained by sampling within one-half square meter sampling frames by D-vac for foliage-inhabiting spiders, followed immediately by hand search for those on the soil surface. Very small numbers of spiders were collected throughout the autumn, exclusively on the soil surface at first, but appearing later also on the foliage. The families Lycosidae and Gnaphosidae predominated. Aphids included the corn leaf aphid and bird cherry-oat aphid.

Integrated pest management.

Multitrophic interactions. Modified cultural practices and control of volunteer wheat and barley can be important in minimizing Russian wheat aphid infestations. However, chemical control is the primary method used. The effect of low concentrations of the newly developed systemic aphicides CGA 215944 and Gaucho[theta] (imidacloprid) on the feeding behavior of the RWA on wheat was evaluated using electronic feeding monitors. Aphids began surviving 15 days after treatment with CGA 215944 (soil drench, 10 g ai/l) and 45 days after planting on plants grown from seed treated with Gaucho (1 fluid oz/cwt). Following these periods of acute toxicity, CGA 215944 exhibited antifeedant effects on D. noxia that were expressed by changes in the frequency and duration of different feeding behaviors. Aphids on plants treated with CGA 215944 spent significantly more time in nonprobing activities and had a much higher frequency of leaf penetration, followed by significantly shorter periods of phloem ingestion. Immediately after the period of acute aphid toxicity, there were no substantial carryover effects from CGA 215944 or Gaucho on the tritrophic relationship between host plant, D. noxia, and Diaeretiella rapae McIntosh.

RWA genetics. Eleven RWA clones, collected from five states (Colorado, Kansas, Nebraska, Wyoming, and Washington) from 1994-95, were evaluated for biotypic variation on four wheat (Pavon, PI 372129, STARS-9302W, and STARS-9501W) and two barley (Wintermalt and STARS-9301B) germplasm entries. Although statistical differences were detected in measurements of chlorosis, leaf rolling, leaf number, tiller number, plant height, shoot weight, root weight, aphid numbers, and aphid weight among some clones, no clear differences in virulence properties were apparent. Host responses to RWA infestation among the 11 clones were similar. In addition, RAPD profiles of these 11 clones (plus several others from Colorado, Kansas, and Oklahoma) revealed no variation. We also assayed these clones for variation in the 18S region of the rRNA cistron, but found none. All the clones tested were genotypically and phenotypically equivalent. Ten years after its introduction to the U.S. and before the commercial release of resistant cultivars, the RWA has not exhibited any biotypic variation. Because of the threat of other introductions, RWA should be periodically monitored for biotypic variation, before and after the deployment of resistant cultivars.

Evaluation of alternate hosts and determination of economic injury levels. Greenbugs collected over 35 counties in Oklahoma from 1995-96 wheat crops were identified to biotype and also to susceptibility to phosphate insecticides. Biotype K, a recently identified greenbug biotype that will kill biotype I-resistant grain sorghum germplasm, was identified in greenbug collections from 12 Oklahoma counties. This is important information, because commercial grain sorghum companies are currently developing biotype I-resistant grain sorghum varieties. Several of the greenbug collections were resistant to phosphate insecticides. In cooperation with Kansas State University scientists, work will continue to determine the distribution of biotype K in the Midwest and to monitor the greenbug population for insecticide resistance.

We completed a one-season test on the economic threshold of greenbugs on winter wheat. Current thresholds are outdated, because none relate aphid numbers/tiller to yield loss, control costs, and monetary value of the wheat. Our studies utilized control cost, monetary value of the crops, and number of aphids/tiller. We will continue to evaluate the economic injury level of greenbugs and other cereal aphids.

Different species of wheatgrasses continue to be evaluated for resistance to the RWA. The mechanisms of the resistance identified in the wheatgrass species also are being identified. We recently evaluated all available crested wheatgrass entries and results have been submitted to the Journal of Economic Entomology. Western wheatgrass entries are currently being tested for resistance to the RWA.

The known greenbug biotypes, B, C, E, F, G, H, I, and K, are being evaluated against several species of grasses to determine if different levels of susceptibility occur among the grass species. Canada bluegrass is susceptible to biotype F, although resistant to biotypes B, C, and E. No information exists on the newer biotypes and their interaction with forage grasses.

Personnel.

Dr. Dirk B. Hays has recently joined us as postdoctoral research associate, and will be working on the biochemistry and physiology of aphid resistance in plants.

Publications.

Baker CA, Porter DR, and Webster JA. 1996. Inheritance and mechanisms of Russian wheat aphid (Homoptera: Aphididae) resistance in PI 225217. Proc 20th Hard Red Winter Wheat Work Conf p. 106.

Baker CA, Porter DR, and Webster JA. 1996. Identification of genetic diversity for Russian wheat aphid resistance in wheat. Agron Abstr p. 93.

Burd JD and Elliott NC. 1996. Changes in chlorophyll a fluorescence induction kinetics in cereals infested with Russian wheat aphid (Homoptera: Aphididae). J Econ Entomol 89:1332-1337.

Burd JD and Elliott NC. 1995. The effect of Russian wheat aphid feeding chlorophyll a fluorescence induction kinetics of resistant and susceptible cereals. Eur J Plant Pathol, Proc 13th Inter Plant Prot Cong, The Hague, Abstracts. Abstr 1005.

Burd JD, Elliott NC, and Reed DK. 1996. Effects of the aphicides 'Gaucho' and CGA-215944 on feeding behavior and tritrophic interactions of Russian wheat aphids. Southwest Entomol 21:145-152.

Burd JD, Webster JA, Puterka GJ, Hoxie RP, and Wellso SG. 1996. Effect of Russian wheat aphid on constituent nonstructural carbohydrate content in wheat seedlings. Southwest Entomol 21:167-172.

Elliott NC and Michels Jr, GJ. 1997. Estimating aphidophagous coccinellid populations in alfalfa. Biological Control 8:43-51.

Elliott NC, Burd JD, Butts RA, Lee JH, Shufran KA, Hein GL, Armstrong JS, Holtzer TO, Peairs FB, and Webster JA. 1997. Biology, ecology, and Management of the Russian wheat aphid. In: Ecologically-Based IPM in Dryland Cropping Systems, Proceedings of a Workshop (Holtzer TO and Peairs FB comp). Colorado State University, Fort Collins. (In Press).

Elliott NC, Kieckhefer RW, and Obrycki JJ. 1996. The influence of within patch and landscape factors on aphidophagous insect communities in wheat. Program and Abstracts 11th Ann U.S. Landscape Ecol Symp. p. 42.

French BW and Elliott NC. 1996. Permeability of grassland-wheat field borders to ground beetles (Carabidae). Program and Abstracts 11th Ann U.S. Landscape Ecol Symp. p. 49.

Gatschet MJ, Taliaferro CM, Porter DR, Anderson MP, Anderson JA, and Jackson KW. 1996. A cold-regulated protein from bermudagrass crowns is a chitinase. Crop Sci 36:712-718.

Greenstone MH. 1996. Serological analysis of arthropod predation: past, present and future. In: The ecology of agricultural pests-biochemical approaches (Symondson WOC and Liddell E eds). Chapman and Hall, London. Pp. 265-300.

Kindler SD and Hammon RW. 1996. Comparison of host suitability of western wheat aphid with the Russian wheat aphid. J Econ Entomol 89(6):1621-1630.

Michels GJ, Jr, Elliott NC, Romero RL, and Johnson TD. 1996. Sampling aphidophagous Coccinellidae in grain sorghum. Southwest Entomol 21:237-246.

Miller HL and Porter DR. 1996. A technique to quantitatively measure the leaf streaking symptom of Russian wheat aphid infestation. Crop Sci 37:278-280.

Mornhinweg DW, Porter DR, and Webster JA. 1996. Effect of constant Russian wheat aphid (RWA) infestation on yield and yield components of RWA-resistant and -susceptible barleys. Agron Abstr p. 93.

Porter DR, Burd JD, Shufran KA, and Webster JA. 1996. Effect of wheat and sorghum resistance genes on the development of new greenbug biotypes. Agron Abstr p. 79.

Webster JA, Porter DR, Burd JD, and Mornhinweg DW. 1996. Effect of growth stage of resistant and susceptible barley on the Russian wheat aphid, Diuraphis noxia. J Agric Entomol 13(4):283-291.

Wratten SD, Elliott NC, and Farrell J. 1995. In: Integrated pest management in Wheat (Dent D ed). Chapman & Hall, London. pp. 241-279.


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