Summary report on cooperative evaluation of the wild Triticum and Aegilops species.

W.J. Raupp, D.L. Wilson, and B.S. Gill.

The table below includes an update of all screening by the WGRC with recognition to the following collaborators: J.H. Hatchett, USDA-ARS, Kansas State University-Hessian fly; S.L. Jones, University of Washington-stem rust; S.A. Harrison, Louisiana State University-bacterial streak;.S.L. Leath, North Carolina State University-powdery mildew; T.L. Harvey, Kansas Agricultural Experiment Station-greenbug and Russian wheat aphid; R. Eastwood, University of Victoria-cereal cyst nematode; and J. Thomas, Agriculture Canada, Leftbridge-wheat curl mite.

_______________________________________________________________________________

# Lines % of total % of total

Pest/species tested # resistant tested resistant tested

_______________________________________________________________________________

Bacterial streak Xanthomonas campestris

Ae. tauschii 253 40 15.8 73.0

Fusarium head blight Fusarium tritici

Ae. tauschii 87 0 0.0 23.5

Leaf rust PRTUS6 (Resistance based on a scale of 0-9 with plants rating < 2 scored as resistant)

T. monococcum

ssp. aegilopoides 579 184 31.8 98.5

T. urartu 194 64 33.0 99.5

T. armeniacum 306 94 30.7 99.7

T. dicoccoides 378 9 2.4 98.2

Other Triticum1 134 30 22.4 95.0

Ae. tauschii 341 63 18.5 98.3

Other Aegilops2 260 149 57.3 41.6

Powdery mildew (Resistance based on a scale of 0-9 with plants rating < 3 scored as resistant)

Isolate ABK/127

T. dicoccoides 3 1 33.3 0.1

Ae. tauschii 116 15 12.9 34.0

Other Aegilops2 194 153 78.9 31.0

_______________________________________________________________________________

# Lines % of Total % of Total

Pest/species tested # Resistant tested resistant tested

_______________________________________________________________________________

Powdery mildew (continued)

Isolate YumaCC/Asosan

Ae. tauschii 115 12 10.4 33.7

Other Aegilops2 169 133 78.7 27.1

Isolate Quincy/Mo10

T. dicoccoides 3 1 33.3 0.1

Other Aegilops2 35 22 62.9 5.6

Steve Leath's Isolate #2

Ae. tauschii 177 21 11.9 51.2

Steve Leath's Isolate 7-12

Ae. tauschii 177 24 13.6 51.2

Steve Leath's Isolate #9

T. timopheevii

ssp. armeniacum 265 75 28.3 88.0

Scab Fusarium gramineum

Ae. tauschii 100 0 0.0 27.0

Septoria leaf blotch Septoria tritici (Resistance based on a scale of 0-9 with plants rating

< 3 scored as resistant)

T. timopheevii

ssp. armeniacum 110 110 35.8 100.0

Stem rust (Resistance based on a scale of 0-5 with plants rating < 3 scored as resistant)

Isolate TMN

T. timopheevii

ssp. armeniacum 324 20 6.2 6.2

Tan spot (Resistance in T. dicoccoides determined on field-grown plants. Plants with no lesions

scored as resistant. Other species screened in the greenhouse using a Hagborn device to inject the

tan spot toxin into the leaves. Resistance based on a scale of 1-5, with plants rating a 1 scored as

resistant.)

T. timopheevii

ssp. armeniacum 110 91 82.7 35.8

T. dicoccoides 18 0 0.0 4.7

Ae. tauschii 326 208 63.9 94.0

Wheat streak mosaic virus (Plant score based on symptomology and ELISA ratings)

T. monococcum

ssp. aegilopoides 12 0 0.0 42.7

T. timopheevii

ssp. armeniacum 136 0 0.0 45.8

T. dicoccoides 174 0 0.0 16.6

_______________________________________________________________________________

# Lines % of total % of total

Pest/species tested # resistant tested resistant tested

_______________________________________________________________________________

Wheat streak mosaic virus (continued)

Ae. tauschii 80 0 0.0 46.0

Other Aegilops2 36 0 0.0 5.7

Hessian fly (Plants scored as resistant, segregating, and susceptible. The number of resistant

plants includes those lines segregating for resistant and susceptible plants.)

Biotype D

T. monococcum

ssp. aegilopoides 258 73 28.3 42.6

T. urartu 72 45 62.5 36.9

T. timopheevii

ssp. armeniacum 141 128 90.8 45.9

T. dicoccoides 181 12 6.6 47.0

Other Triticum1 7 0 0.0 5.0

Ae. tauschii 316 188 59.5 91.1

Other Aegilops2 207 68 32.9 33.1

Biotype GP

Ae. tauschii 54 28 51.9 15.8

Greenbug biotype E (Resistance based on a scale of 0-9 with plants rating < 2 scored as

resistant)

T. monococcum

ssp. aegilopoides 407 48 11.8 67.2

T. urartu 130 1 0.8 66.2

T. timopheevii

ssp. armeniacum 194 4 2.1 63.2

T. dicoccoides 298 6 2.0 77.4

Other Triticum1 86 0 0.0 61.0

Ae. tauschii 269 162 60.2 77.5

Other Aegilops2 234 28 12.0 37.5

Russian wheat aphid (PI372179 was the resistant control. TAM107 was the susceptible

control and is the cultivar with the most resistance known at this time. Resistant plants rated

< 4.5 on a 1-9 scale.)

T. monococcum

ssp. aegilopoides 149 17 11.4 24.8

T. urartu 169 2 1.2 86.7

T. timopheevii

ssp. armeniacum 116 22 19.0 37.9

T. dicoccoides 96 0 0.0 24.9

T. monococcum 22 8 36.4 78.6

Ae. tauschii 233 1 0.4 68.3

_______________________________________________________________________________

# Lines % of total % of total

Pest/species tested # resistant tested resistant tested

_______________________________________________________________________________

Wheat curl mite

T. urartu 142 2 1.4 72.8

T. dicoccoides 299 1 0.3 77.7

T. monococcum

ssp. aegilopoides 96 2 2.0 15.8

T. timopheevii

ssp. armeniacum 241 20 8.3 80.3

Ae. tauschii 323 63 19.5 92.3

Cereal cyst nematode (Resistance based on a scale of 0-5 with plants rating < 2 scored as

resistant)

Ae. tauschii 302 3 0.01 86.0

Winterhardiness (Evaluation for winter damage was in late March at two Manhattan

locations. Winterhardiness with > 80 % survival of each accession were considered cold

tolerant.)

T. monococcum

ssp. aegilopoides 20 19 95.0 3.3

T. urartu 20 20 100.0 10.2

T. timopheevii

ssp. armeniacum 20 20 100.0 6.7

T. dicoccoides 19 11 58.0 5.0

Ae. tauschii 214 77 36.0 61.8

Other Aegilops2 157 93 59.2 25.1

_______________________________________________________________________________

1This category includes T. monococcum, T. carthlicum, and other tetraploid and hexaploid wheats.

2This category includes all other species classified as Aegilops, excluding Ae. tauschii.

The Wheat Genetics Resource Center on the World Wide Web.

W.J. Raupp.

The Wheat Genetics Resource Center now has a site on the World Wide Web. The address is: http://www.ksu.edu/~raupp. Currently, the site includes data on all of the WGRC germ plasm releases, personnel records and lists of publications from 1993-96. Many of the listed publications from 1995-96 include abstracts. We hope to have pertinant data on the WGRC germ plasm collection and pest evaluations accessible there in the near future.

Publications.

Badaeva ED and Gill BS. 1995. Spontaneous chromosome substitutions in hybrids of Triticum aestivum with T. araraticum detected by C-banding technique. Wheat Inf Serv 80:26-31.

Badaeva ED, Gill BS, Badaev NS, Kawahara T, and Filatenko AA. 1995. Chromosomal rearrangements and the process of intraspecific diversity in Triticum araraticum. In: Proc 8th Inter Wheat Genet Symp (Li ZS and Xin ZY eds), Beijing, China. China Agriculture Scientech Press, Beijing. pp. 293-298.

Badaeva ED, Jiang J, and Gill BS. 1995. Detection of intergenomic translocations with centromeric and noncentromeric breakpoints in Triticum araraticum: mechanism of origin and adaptive significance. Genome 38:976-981.

Boyko E, Gill KS, Hassawi D, Fritz AK, Namuth D, Lapitan NLV, Cox TS, Sears RG, and Gill BS. 1995. A diploid genetic map of wheat based on Triticum tauschii (2n = 14), the D genome progenitor of bread wheat. Plant Genome III Abstracts. p. 27.

Cabrera A, Friebe B, Jiang J, and Gill BS. 1995. Characterization of Hordeum chilense chromosomes by C-banding and in situ hybridization using highly repeated DNA probes. Genome 38:435-442.

Cox TS. 1995. Simultaneous selection for major and minor resistance genes. Crop Sci 35(3):1337-1346.

Cox TS, Gill BS, and Sears RG. 1995. Notice of release of KS94WGRC32 leaf rust-resistant hard red winter wheat germplasm. Fall Cereals Conf, 5-6 August, 1994, Manhattan, KS. p. 26.

Cox TS, Bockus WW, Gill BS, Sears RG, Heer WF, and Long JH. 1995. Notice of release of KS95WGRC33 hard red winter wheat germplasm resistant to Septoria leaf blotch. Fall Cereals Conf, 3-4 August, 1995, Manhattan, KS. (In press).

Cox TS, Gill BS, Sears RG, Harvy TL, Hatchett JH, Hulbert SH, Boyko EV, Brown-Guedira, GL, Delaney DE, Friebe B, Gill KS, Jiang J, Knackstedt MA, Raupp WJ, and Van Metteren ND. 1995. Research summary. Ann Wheat Newslet 41:241-246.

Delaney DE, Friebe B, Hatchett JH, Gill BS, and Hulbert SH. 1995. Targeted mapping of a Hessian fly-resistance gene from rye by representational difference analysis. Plant Genome III Abstracts. p. 33.

Delaney DE, Friebe B, Hatchett JH, Gill BS, and Hulbert SH. 1995. Targeted mapping of a Hessian fly-resistance gene from rye by representational difference analysis. Genome 38:458-466.

Delaney DE, Nasuda S, Endo TR, Gill BS, and Hulbert SH. 1995. Cytologically based physical maps of the group-2 chromosomes of wheat. Theor Appl Genet 91:568-573.

Delaney DE, Nasuda S, Endo TR, Gill BS, and Hulbert SH. 1995. Cytologically based physical maps of the group-3 chromosomes of wheat. Theor Appl Genet 91:780-782.

Deol GS, Wilde GE, and Gill BS. 1995. Host plant resistance in some wild wheats to the Russian wheat aphid, Diuraphis noxia (Mordvildo) (Homoptera: Ahididae). Plant Breeding 114.

Endo TR and Gill BS. 1995. Production of deletion stocks in wheat. In: Proc 8th Inter Wheat Genet Symp (Li ZS and Xin ZY eds), Beijing, China. China Agriculture Scientech Press, Beijing. pp. 211-216.

Endo TR. and Gill BS. 1995. The deletion stocks of common wheat. J Hered (In press).

Friebe B, Badaeva ED, and Gill BS. 1996. Standard karyotypes of Triticum uniaristatum, T. tripsacoides, T. comosum, and T. heldreichii (Poaceae). Euphytica (In press).

Friebe B and Gill BS. 1996. Chromosome banding and genome analysis in diploid and cultivated polyploid wheats. In: Methods of Genome Analysis (Jauhar PP ed). CRC Press, Boca Raton, FL. pp. 39-60.

Friebe B, Gill BS, Tuleen NA, and Cox TS. 1995. Registration of KS93WGRC28 powdery mildew resistant T6BS-6RL hard red winter wheat germplasm. Crop Sci 35:1237.

Friebe B, Gill KS, Tuleen NA, and Gill BS. 1996. A new Agropyron intermedium-derived source for wheat streak mosaic virus resistance and its transfer to common wheat. Euphytica (In press).

Friebe B, Jiang J, Tuleen NA, and Gill BS. 1994. Standard karyotype of Triticum umbellulatum and the characterization of derived chromosome addition and translocation lines in common wheat. Theor Appl Genet 90:150-156.

Friebe B, Jiang J, Badaeva ED, Raupp WJ, McIntosh RA, and Gill BS. 1996. Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica (In press).

Friebe B, Jiang J, and Gill BS. 1995. Detection of 5S rDNA loci and other repetitive DNA sequences on supernumerary B chromosomes of Triticum species. Pl Syst Evol 196:131-139.

Friebe B, Jiang J, Raupp WJ, and Gill BS. 1995. Molecular cytogenetic analysis of radiation-induced alien genetic transfers in wheat. In: Proc 8th Inter Wheat Genet Symp (Li ZS and Xin ZY eds), Beijing, China. China Agriculture Scientech Press, Beijing. pp. 519-529.

Friebe B, Tuleen NA, and Gill BS. 1995. Standard karyotype of Triticum searsii and its relationship with other S-genome species and common wheat. Theor Appl Genet 91(2):248-254.

Friebe B, Zhang W, Raupp WJ, Gill BS, and Porter D. 1995. Non-homologous wheat-rye chromosomal translocations conferring resistance to greenbug. Euphytica 84:121-125.

Fritz AK, Cox TS, Gill BS, and Sears RG. 1995. Molecular marker-facilitated analysis of introgression in winter wheat x Triticum tauschii populations. Crop Sci 35:1691-1695.

Fritz AK, Cox TS, Gill BS, and Sears RG. 1995. Molecular-based analysis of quantative traits in winter wheat x Triticum tauschii populations. Crop Sci 35:1696-1699.

Gill BS. 1995. Molecular cytogenetics: integration of C-banding, in situ hybridization, and RLFPs in genome mapping and crop improvement of wheat. In: Proc 8th Inter Wheat Genet Symp (Li ZS and Xin ZY eds), Beijing, China. China Agriculture Scientech Press, Beijing. pp. 41-47.

Gill BS. 1995. Molecular cytogenetics: an integrated approach for evolutionary, structural and functional analysis of plant chromosomes. In: Plant Genome and Plastome: their structure and evolution (Tsunewaki K ed). Kodansha Scientific Ltd., Tokyo, Japan. pp. 23-32.

Gill BS. 1995. The molecular cytogenetic analysis of economically important traits in plants. In: Kew Chromosome Conf. III. (In press).

Gill BS, Gill KS, and Friebe B. 1995. Cytogenetic laddermaps and cereal chromosome structure, function, and manipulation. In: Classical and Molecular Cytogenetic Analysis. Proceedings of a U.S.-Japan Symposium (Raupp WJ and Gill BS eds). Department Report, Dept. Plant Pathology, Kansas State Univ., Manhattan. pp. 129-135.

Gill BS, Friebe B, Wilson DL, Martin TJ, and Cox TS. 1995. Registration of KS93WGRC27 wheat streak mosaic virus-resistant hard red winter wheat germplasm. Crop Sci 35:1236-1237.

Hetrick BAD, Wilson GWT, Gill BS, and Cox TS. 1995. Chromosome location of mycorrhizal responsive genes in wheat. Can J Bot 73:891-897.

Hohmann U, Endo TR, Herrmann RG, and Gill BS. 1995. Characterization of deletions in common wheat induced by an Aegilops cylindrica chromosome: detection of multiple chromosome rearrangements. Theor Appl Genet 91:611-617.

Hohmann U, Graner A, Endo TR, Gill BS, and Herrmann RG. 1995. Comparison of wheat physical maps with barley linkage maps for group 7 chromosomes. Theor Appl Genet 91:618-626.

Hsam SLK, Cerme-o MC, Friebe B, and Zeller FJ. 1995. Transfer of Amigo wheat powdery mildew resistance gene Pm17 from T1AL-1RS to the T1BL-1RS wheat-rye translocated chromosome. Heredity 74:497-501.

Jiang J, Friebe B, and Gill BS. 1994. Chromosome painting of Amigo wheat. Chromosoma 89:811-813.

Jiang J, Gill BS, Wang GL, Ronald PC, and Ward DC. 1995. Metaphase and interphase FISH mapping of the rice genome using bacterial artificial chromosomes. Proc Nat Acad Sci USA 92:4487-4497.

Liu DJ, Chen PD, and Raupp WJ. 1995. Determination of homoeologous groups of Haynaldia villosa chromosomes. In: Proc 8th Inter Wheat Genet Symp (Li ZS and Xin ZY eds), Beijing, China. China Agriculture Scientech Press, Beijing. Pp. 181-185.

McIntosh RA, Friebe B, Jiang J, The D, and Gill BS. 1995. Cytogenetical studies in wheat. WVI. Chromosome location of a new gene for resistance to leaf rust in a Japanese wheat-rye transloction line. Euphytica 82:141-147.

Martin TJ and Harvey TL. 1995. Notice of release of Russian wheat aphid resistant wheat germplasms. Fall Cereals Conf, 5-6 August, 1994, Manhattan, KS. p. 25.

Mickelson-Young LA, Endo TR, and Gill BS. 1995. Cytogenetic laddermaps of the group 4 chromosomes of wheat. Theor Appl Genet 90:1007-1011.

Raupp WJ, ed. 1995. Annual Wheat Newsletter. Department Report, Dept. Plant Pathology, Kansas State Univ., Manhattan. 358 pp.

Raupp WJ and Gill BS, eds. 1995. Classical and Molecular Cytogenetic Analysis. Proceedings of a U.S.-Japan Symposium. Department Report, Kansas State Univ., Manhattan. 217 pp. Price $25 US.

Raupp WJ, Friebe B, and Gill BS. 1995. Suggested guidelines for the nomenclature and abbreviation of the genetic stocks of wheat, Triticum aestivum L. em Thell. and its relatives. Wheat Inf Serv 81:51-55.

Raupp WJ, Gill BS, Friebe B, Wilson DL, Cox TS, and Sears RG. 1995. The Wheat Genetics Resource Center: germ plasm conservation, evaluation, and utilization. In: Proc 8th Inter Wheat Genet Symp (Li ZS and Xin ZY eds), Beijing, China. China Agriculture Scientech Press, Beijing. pp. 459-466.

Sieffers DL, Martin TJ, Harvey TL, and Gill BS. 1995. Temperature sensitivity and efficacy of wheat streak mosaic virus resistance derived from Agropyron intermedium. Plant Dis 79:1104-1106.

Singh S, Gill KS, Cox TS, and Gill BS. 1995. An RFLP-based inter- and intra-chromosomal mapping of disease resistance gene(s) in wheat. In: Proc 8th Inter Wheat Genet Symp (Li ZS and Xin ZY eds), Beijing, China. China Agriculture Scientech Press, Beijing. pp. 557-562.

Sutka J, Farshadfar E, Köszegi B, Friebe B, and Gill BS. 1995. Drought tolerance of disomic chromosome additions of Agropyron elongatum to Triticum aestivum. Cereal Res Commum 23:351-357.

Van Deynze AE, Dubcovsky J, Gill KS, Nelson JC, Sorrells ME, Dvorak J, Gill BS, Lagudah ES, McCouch SR, and Appels R. 1995. Molecular-genetic maps for group 1 chromosomes of Triticeae species and their relation to chromosomes in rice and oat. Genome 38:45-59.

Zhang J, Friebe B, Zhang W, and Gill BS. 1995. Detection of maize-wheat cross-hybridizing DNA sequences in wheat chromosomes. Genome 38:946-950.


U.S. GRAIN MARKETING AND PRODUCTION RESEARCH CENTER

USDA, Agricultural Research Service, Manhattan, KS 66502, USA.

O.K. Chung, G.L. Lookhart, J.L. Steele, L.M. Seitz, C.S. Chang, I.Y. Zayas, S.R. Bean, Y.S. Kim, J.B. Ohm, D.B. Bechtel, D.W. Hagstrum, D.B. Sauer, P.W. Flinn, J.E. Baker, K.J. Kramer, A.K. Dowdy, F.H. Arthur, C.R. Martin, R.W. Howard, C.E. Lang, H.S. Park, J.H. Brower, M.S. Caley, B.W. Seabourn, J.D. Hubbard, W.H. McGaughey, T.D. Morgan, J.E. Throne, and D.E. Walker