NEW YORK

CORNELL UNIVERSITY

Department of Plant Breeding and Biometry, Ithaca, NY 14853-1901, USA.

M.E. Sorrells.

1995 winter wheat production.

The 1995 soft white winter wheat crop for New York was 125,000 acres, slightly below average for the state. Yield was estimated to be 55 b/acre, the second highest average yield on record. For 1995, temperatures were near normal, but precipitation was 13 cm below normal for April through July. Precipitation was well below normal for every month.

Soft white winter wheat.

Batavia, Harus, Geneva, and Pioneer Brand 2737W are soft white winter cultivars currently recommended to New York farmers. Limited quantities of certified seed of Cayuga (NY262-37-422) were available this past fall. Geneva Reselect (reselection of Geneva) has been approved for release. This line has shown exceptionally high yield for the past 4 years. Although it was found in a Foundation seed field of Geneva, it is much shorter and has white instead of bronze chaff.

Soft red winter wheat.

Soft red cultivars that are performing well in New York state trials include Hoffman 89 and Pioneer Brands 2548, 2545, and 2510. The soft red winter market class continues to gain in popularity, especially following years when considerable damage was caused by preharvest sprouting in the white wheats. We tested our soft red entries for sprout resistance this past year for the first time. Those entries showing above-average sprouting damage were Cardinal, CL860434, C4227, and Casey. Also, Presto triticale showed more damage than most reds. Spelt varieties ranged from very resistant to very susceptible. We have initiated a fee testing program for private varieties. The trials are grown in four locations, and the fee is $200 per entry.

Hard red spring wheat.

Production of hard red spring wheat continues at a low level. Stoa continues to be the most popular variety.

RFLP mapping.

Our research program currently is focussed on comparative mapping. We developed consensus maps for members of the Triticeae tribe (T. aestivum, T. tauschii, and Hordeum spp.) and compared them to maps for rice, maize, and oat. The aneuploid stocks in wheat have been invaluable to comparative mapping, because almost every DNA fragment can be allocated to a chromosome arm, thus preventing erroneous conclusions about probes that could not be mapped because of the lack of polymorphism between mapping parents. The orders of markers detected by probes mapped in rice, maize, and oat were conserved for 93, 92, and 94 % of the length of Triticeae consensus maps, respectively. The duplicated chromosome segments within the maize genome, because of ancient polyploidization, were identified by homoeology of segments from two maize chromosomes to regions of one Triticeae chromosome. Homoeologous segments conserved across Triticeae species, rice, maize, and oat were identified for each Triticeae chromosome.

We have identified a set of cDNA probes to map as anchor loci across rice, wheat, oat, and maize. These probes hybridized across several grass genera and demonstrated their efficacy for comparative mapping. The anchor probes were used to extend the existing RFLP linkage maps of rice, maize, wheat, oat, sorghum, and sugarcane and to compare map structure between these crops. Comparisons based on the map position of anchor probes demonstrated the collinearity of cDNA markers across the genera and revealed conserved rearrangements and duplications. Correspondence of QTL with qualitative genes in homoeologous chromosome segments of other species indicate that the genes underlying many of these traits may be orthologous.

Wheat spindle streak mosaic virus resistance.

In collaboration with Dr. G. Bergstrom, we developed a recombinant inbred population from the cross `Geneva/Augusta' for the purpose of mapping resistance to WSSMV. We have completed 3 years of evaluation of visual symptoms of this virus, and it appears to segregate as a single gene. RFLP mapping has been initiated.

Milling and baking.

We have completed evaluation of the `Clark's Cream/NY6432-18' mapping population for milling and baking quality traits with the goal of mapping those genes. We have identified genes controlling flour protein concentration, alkaline water retention capacity, and flour yield. We also are analyzing mixing strength. This is a colloborative project with the Wooster Soft Wheat Quality Lab, Jim Anderson, and Kim Campbell.

Publications.

Autrique E, Nachit MM, Monneveux P, Tanksley SD, and Sorrells ME. 1996. Genetic diversity in durum wheat based on RFLPs, morphophysiological traits and coefficient of parentage. Crop Sci 36:735-742.

Autrique E, Singh RP, Tanksley SD, and Sorrells ME. 1995. Molecular markers for four leaf rust resistance genes introgressed into wheat from wild relatives. Genome 38:75-83.

da Silva J and Sorrells ME. 1996. Linkage analysis in polyploids using molecular markers. In: Methods of Genome Analysis in Plants: Their Merits and Pitfalls (Jauhar P ed). CRC Press, Inc., Boca Raton, FL.

Ma AQ, Zhao Y-H, and Sorrells ME. 1995. Inheritance and chromosomal location of a male fertility restoring gene transferred from Aegilops umbellulata Zhuk. to Triticum aestivum L. Mol Gen Genet 247:351-357.

Ma ZQ, Roder MS, and Sorrells ME. 1996. Frequencies, and sequence characteristics of microsatellites in wheat. Genome (In press).

Ma ZQ and Sorrells ME. 1995. Genetic analysis of fertility restoration in wheat using restriction fragment length polymorphism. Crop Sci 35:1137-1143.

Nelson JC, Van Deynze AE, Autrique E, Sorrells ME, Lu YH, Merlino M, Atkinson M, and Leroy P. 1995. Molecular mapping of wheat. Homoeologous group 2. Genome 38:517-524.

Nelson JC, Van Deynze AE, Autrique E, Sorrells ME, Lu YH, Negre S, Bernard M, and Leroy P. 1995. Molecular mapping of wheat. Homoeologous group 3. Genome 38:525-533.

Nelson JC, Sorrells ME, Van Deynze AE, Lu YH, Atkinson M, Bernard M, Leroy P, Faris JD, and Anderson JA. 1995. Molecular mapping of wheat. Homoeologous groups 4, 5, and 7. Genetics 141:721-731.

Roder MS, Plaschke J, Konig SU, Boner A, Sorrells ME, Tanksley SD, and Ganal MW. 1995. Abundance, variability, and chromosomal location of microsatellites in wheat. Mol Gen Genet 246:327-333.

Sorrells ME, Van Deynze A, Nelson JC, and McCouch SR. 1995. Comparative mapping in the Gramineae. In: Classical and Molecular Cytogenetic Analysis. Proc US-Japan NSF Symp (Raupp WJ and Gill BS eds). Department Report, Dept. Plant Pathology, Kansas State Univ. Manhattan Pp. 76-84.

Sorrells ME and Anderson JA. 1996. Quantitative trait loci associated with preharvest sprouting in white wheat. In: Seventh Inter Symp on Preharvest Sprouting in Cereals, July 2-7, 1995, Abashiri, Japan.

Van Deynze AE, Dubcovsky J, Gill KS, Nelson JC, Sorrells ME, Dvorak J, Gill BS, Lagudah ES, McCouch SR, and Appels R. 1995. Molecular-genetic maps for group 1 chromosomes of Triticeae species and their relation to chromosomes in rice and oat. Genome 38:45-59.

Van Deynze AE, Nelson JC, Yglesias ES, Harrington SE, Braga DP, McCouch SR, and Sorrells ME. 1995. Comparative mapping in grasses. Wheat relationships. Mol Gen Genet 248:744-754.

Van Deynze AE, Nelson JC, O'Donoughue LS, Ahn SN, Siripoonwiwat W, Harrington SE, Yglesias ES, Braga DP, McCouch SR, and Sorrells ME. 1995. Comparative mapping in qrasses. Oat relationships. Mol Gen Genet 249:349-356.