Double haploid laboratory.

The laboratory specialises in cereals and delivered 11,000 dihaploid lines, in the form of seeds, to breeders in 1994. The techniques are well mastered at present, and the laboratory offers its services to outside breeders. The main advantages are reduction of time and increased ease of breeding.

Marketing.

Production of HYNO-PRECIA was scaled up significantly in 1994. This hybrid variety was multiplied with the use of our Chemical Hybridizing Agent (CHA), Croisor, on 10 fields. This represents a total area of 12 hectares (female part only). Good sterility was obtained with an average purity level of 98.2 %. (The average gross F1 seed yield/hectare was 5 tons).

In 1995, production of this hybrid will surpass 100 hectares. Two other varieties that have entered into second-year registration also will be multiplied. To reach our development goals, we have reinforced our production team, which now comprises four production technicians.

HYNO-PRECIA's registration, on October 19, 1994, has allowed us to sell 700 doses of 750,000 grains each. Commercialization of hybrid seed for 1994 constituted our first market test. It should help us define more precisely our market position next fall.

All development work is carried out in close relationship with agricultural cooperatives and private seed companies, which contribute their know-how and industrial equipment. Overall, 20 French companies are backing HYBRINOVA's project.

HYBRINOVA has achieved, this year, a new level in its development with the registration of hybrid wheat varieties and the beginning of a marketing strategy. Hybrid seed multiplication has begun and will increase in the following years to assume its rightful place in the business.


HYBRITECH SEED INTERNATIONAL, INC.

5912 N. Meridian, Wichita, KS, 67204 USA.

REORGANIZATION: The entire company has been reorganized to focus more clearly on our business objectives. We have moved to a team-based environment, with three teams devoted to specific market class objectives, i.e., Hard Winter, Soft Red Winter, and White Winter, and one Resource and Support Team for administration, business, and technical support functions. A realignment of people and resources was done to reflect the relative hybrid market potential of each of the market classes. Each market class team has Technology (breeding and CHA research), Marketing, and Production components.

New technology.

John Erickson - Wichita, KS, USA.

A new emphasis was initiated to incorporate new technology to assist in achieving greater efficiency in breeding, hybridization systems, and hybrid seed production. Current activities relate to incorporating molecular marker systems into the breeding program, new hybridization systems, end-use quality traits, and hybrid seed quality. The introduction of new traits from the Monsanto biotechnology program is also a focus.

White winter breeding.

Hal Lewis and Steve Scherer - Corvallis, OR, USA.

The white winter project to develop hybrids for the Pacific Northwest is being expanded to meet anticipated market potential. New facilities were acquired in western Oregon.

Hybrid yield testing was initiated with several public cooperators. Soft white winter hybrids have been entered in the USDA Regional Trial for the past 3 years and performed the best over all locations each year, exhibiting broad yield stability.

Soft red winter breeding.

Gordon Cisar, Jack Berg, Jamie Bobula, Chris Davis, Roger Leafgren, and Rollin Machtmes - Lafayette, IN, USA.

We are continuing to develop several hybrid wheat candidates for entry into the soft red winter wheat marketplace. The goals for commercial release are to have a 10 bushel per acre advantage over an average of four competitive check varieties and to have hybrid seed production yields of 40 bushels per acre for any candidate hybrid. Current data indicate that each of these commercial candidates approaches or exceeds these targets.

Hybrid testing of approximately 1,800 to 2,200 entries continues on an annual basis. We have made some attempts to monitor intra- and inter- gene pool heterosis and appear to be making some progress in driving the gene

frequencies in these pools apart. These pools are designated as the X, Y, and Z gene pools. Inter- and intra-pool heterosis for the earliest and latest 5-year periods are given below:

_______________________________________________________________________________________

Intra-pool Heterosis Inter-pool Heterosis

-XX- -YY- -ZZ- -XY- -XZ- -YZ-

_______________________________________________________________________________________

Average best-

parent heterosis 102.6 107.6 104.8 103.0 105.1 102.4

(First 5 years)

Average best-

parent heterosis 104.2 105.8 105.0 105.6 106.5 105.0

(Last 5 years)

Net change + 1.6 - 1.8 + 0.2 + 2.6 + 1.4 + 2.6

_______________________________________________________________________________________

Seed production of experimental hybrids in our crossing block nursery continues to average approximately half the yield of our elite line trial planted on the same farm. The standard deviation for the seed yield distribution has a long-term average of approximately 9 bushels per acre.

Long-term seed yield average: 29.0 bushels per acre

Long-term average, elite line trial: 58.8 bushels per acre

SRWW product development.

Dennis Delaney - Lafayette, IN, USA.

We have over 100 on-farm hybrid comparisons planted throughout Illinois, Indiana, and Ohio for harvest in 1995 of two hybrids proposed for release - Quantum 707 and Quantum 708. This will give us an opportunity to see our hybrids on a larger field scale and to collect and receive grower input.

Quantum 707 and Quantum 708 are both awnletted, soft red winter wheat hybrids, developed from HSI pure-line parents, and tested for 4 years in replicated yield trials at multiple locations throughout the Corn Belt. Both have excellent standability and winterhardiness, resistance to stem rust (Puccinia graminis), soilborne mosaic virus, and good tolerance to leaf rust (Puccinia recondita), common in the SRWW region. They express good tolerance to leaf blights and moderate tolerance to powdery mildew (Erysiphe graminis). Quantum 707 is approximately 2 days earlier in maturity than Cardinal, whereas Quantum 708 heads 1 day earlier.

These hybrids have demonstrated good soft wheat milling and baking quality, being equal to or better than most soft red winter wheat cultivars currently grown.

Another benefit of the hybrids has been good yield stability across locations over several years in the Corn Belt.

The following is a 4-year multilocation yield summary:

___________________________________________________________

Northern Southern Test

Entry corn belt corn belt weight

___________________________________________________________

QUANTUM 707 95.8 78.2 AVE

QUANTUM 708 98.8 81.0 GOOD

CARDINAL 82.7 69.8 AVE

PIONEER 2548 83.7 70.6 AVE

___________________________________________________________

We are also conducting further agronomic and management studies including: seeding rate, seed treatment, tillage, planting date, and fertility trials. These are being done at our research locations and in cooperation with several regional universities and our marketing/distribution partners.

To market our products, we are in the process of setting up partnerships with several organizations. This will allow HybriTech Seed to utilize existing strengths - our own research and technical program, plus those strengths already in our target markets, that is, sales and distribution networks.

Our partners are:

Illinois - GROWMARK

Indiana - Beck's Hybrids

Ohio - AGRA: The Andersons, and Wellman Seeds

- Vigoro

We are fortunate to have the opportunity to work with these established organizations, and look forward to a promising future.

Hard winter wheat breeding.

Steve Kuhr, Jerry Wilson, Randy Rich, Gary Greer, and Tony Ruder - Wichita, KS, USA; and Dudley Leaphart - Billings, MT, USA.

In general, 1994 was a good year for testing; we lost only three sites to adverse weather conditions. Because of frequent showers, we were late planting the nursery at Hereford, TX. Because the soil was sticky, the furrows were insufficiently covered and emergence was poor. Two sites in Montana were lost to blowing soil.

HybriTech entries in the SRPN performed well, with hybrids H1706 and H1693 looking especially strong across a wide area. However, because both are susceptible to leaf rust, their potential commercial areas would be somewhat smaller.

A number of experimental hybrids in our preliminary, intermediate, and advanced trials looked quite promising. The actual number of hybrids making it into our advanced trials has been decreasing over the last 4 years, because we have been tightening up the selection criteria.

HRWW product development.

Walter Bates - Mount Hope, KS, USA.

This year we have four hybrids proposed for release in the grower introduction phase of our testing program. Quantum 566, 579, 558, and 547. On-farm trials for Quantum 566 are being conducted in northwest Kansas, northeast Colorado, South Dakota, and Nebraska. Quantum 579 on-farm trials are being conducted in south-central and southwest Kansas, southeast Colorado, Oklahoma, and Texas. Quantum 558 on-farm trials are being conducted in New Mexico (high elevation), and Quantum 547 on-farm trials are being conducted in Montana. The size of the on-farm trials ranges from 1 to 20 acres.

Two experimental hybrids (XH 1706 and XH 1520) are being confirmed and positioned throughout the Hard Red Winter Wheat region. This fall, eight experimental hybrids will be advanced to our confirming and positioning program.

Agronomic and management studies include: seeding rate, seed treatment, planting date, and fertility trials. These trials are being conducted at HybriTech research locations and in cooperation with universities, Collingwood Grain, and our partners.

Production technology.

Dennis Dunphy and Brett Sowers - Lafayette, IN, USA;

Kent Baker and Kevin Jackson - Perryton, TX, USA;

Richard Evans - Corvallis, OR, USA; and

Sally Metz - St. Louis, MO, USA.

Monsanto received an Experimental Use Permit for the GENESIS hybridizing agent for the 1994 season. GENESIS was used on commercial-scale production fields in both the hard red winter and soft red winter production regions to produce seed of several precommercial hybrids. Seed production in both regions was very successful, with excellent yields of high quality seed being produced. Seed that was produced in 1994 has been distributed to growers for field evaluation.

HybriTech expects to treat additional acreage with GENESIS during the 1995 season, and we anticipate that full, unrestricted use of GENESIS will be approved in 1996. We will be working during the next 2 years to establish both production and marketing capability for QUANTUM hybrids.