Wheat microsatellite WMC 25/06/01

wmc(15h4) Plate reference : 15h4
WMC locus number : WMC Length of sequence : 30
Last updated : Jeu 9 nov 2000 at 14:11
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Apparently no insert. Bases 1 to 30 (complement) show homology to 1F2 bases 1 to 30 (30 in longest run, maxScore = 22). Bases 1 to 30 show homology to 15d5 bases 1 to 30 (30 in longest run, maxScore = 22). No substantial microsatellite.
PCR Comments :



Length of sequence : 30  Consensus sequence is :

..

GGATCCCTCG AGGCGCGCCT CGAGGAATTC

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wmc(15a5) Plate reference : 15a5
WMC locus number : WMC Length of sequence : 212
Last updated : Mar 28 Nov 2000 at 14:13
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 4 to 140 (complement) show homology to 14f4 bases 61 to 197 (137 in longest run, maxScore = 81). (CA)30 93 to 152. 15a5 is a duplicate of 14f4. 14f4 has priority.
PCR Comments :



Length of sequence : 212  Consensus sequence is :

..

CATAAGATGC ATGTCCACCG ACCGCTTTGG TTTTTCTCTC GAGAGTCCTC 
CTACCATCAC GAAACCTTTT CCTCCTCCAC ACCGCTGCAG TCCACACACA 
CACACACACA CACACACACA CACACACACA CACACACACA CACACACACA 
CAAATTCGCC GTGGACCACA CCGTCAGTGT CGCGCGTTAC CCGGAGCATT 
GCTAGCATGT AC

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wmc(15b5) Plate reference : 15b5
WMC locus number : WMC Length of sequence : 190
Last updated : Jeu 9 nov 2000 at 14:26
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to insert at both ends (NB 3' linker corrupted) . (CAA)11 7 to 39, (CAA)32 55 to 150. no room for primers.
PCR Comments :



Length of sequence : 190  Consensus sequence is :

..

CGATAACAAC AACAACAACA ACAACAACAA CAACAACAAT AGCAACAACA 
ACAGCAACAA CAACAACAAC AACAACAACA ACAACAACAA CAACAACAAC 
AACAACAACA ACAACAACAA CAACAACAAC AACAACAACA ACAACAACAA 
CACGGGGCGA CGCAGCAGCA GCTCGATGCT CGATCGGCGG

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wmc(15c5) Plate reference : 15c5
WMC locus number : WMC Length of sequence : 286
Last updated : Jeu 9 nov 2000 at 14:11
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 127 to 224 (complement) show homology to 25f6 bases 98 to 195 (98 in longest run, maxScore = 73). No substantial microsatellite.
PCR Comments :



Length of sequence : 286  Consensus sequence is :

..

GTACTGCTGC TAGCTGCCCC TTGTGCCGTC GCTGCTGTTT GCCGCTGCCG 
TCGCCGCCTT CTGCGTCGCT CCTGTTGCTG CCGCTGCTCG CTGCCGCCGC 
TTCTACGGCC GGCCATGGCC GAGCTGTGAG TGTAGTGTGT GTGTGTTCTG 
CATAGTGTGT GTCCGTGCAG CTGGACATGG CCGTGTGCGC CCGTGTGTGC 
TGAGAGCAGC GTGGTGTGTG TGTGTGTGCA GCGTGGTGTG TGAGTGCGCC 
GTGCGTGTGG CCGGCTGGGC TATGGCCAGT AGTGGC

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wmc(15d5) Plate reference : 15d5
WMC locus number : WMC Length of sequence : 30
Last updated : Jeu 9 nov 2000 at 14:11
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Apparently no insert. Bases 1 to 30 show homology to 15h4 bases 1 to 30 (30 in longest run, maxScore = 22). No substantial microsatellite.
PCR Comments :



Length of sequence : 30  Consensus sequence is :

..

GGATCCCTCG AGGCGCGCCT CGAGGAATTC

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wmc(15e5) Plate reference : 15e5
WMC locus number : WMC Length of sequence : 232
Last updated : Jeu 9 nov 2000 at 14:26
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 1 to 232 (complement) show homology to 15h2 bases 1 to 232 (232 in longest run, maxScore = 184). (GGC)6 132 to 149. 15e5is a duplicate of 15h2. 15h2 has priority.
PCR Comments :



Length of sequence : 232  Consensus sequence is :

..

GGACCGTGGT CCAGAATTTT TTGTGTTCCG TAGCTGTGTG CGTGCCCGTT 
GGCTAGGGAA AAAAAGCGTG TGCCCTGTCA GAGGCTGGTT GACGCCTCAG 
AGTGTTGTAT TCCGTGGCGG AGAGCATGCA TGGCGGCGGC GGCGGCGGCG 
AGGGGAGTAT GGAGAATGCC CCTGTCTCAT CTGGGGAGTC GGACAGCCGG 
ACCCGAGATC GCCCGGCTGG ATTGGAAAGT AC

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wmc(15f5) Plate reference : 15f5
WMC locus number : WMC Length of sequence : 144
Last updated : Jeu 9 nov 2000 at 14:11
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 1 to 144 show homology to 14d6 bases 1 to 144 (144 in longest run, maxScore = 115). No substantial microsatellite.
PCR Comments :



Length of sequence : 144  Consensus sequence is :

..

GTACGACGCG AGAGGGGAAG GGAGGAAGGG AGAAGCTCAG CGAGCGGCTA 
CGAAAAGCCT GTGCGAGCTT AGGAGCAACA GAGGTCGACA GAGACGGTGG 
CGGGCGGCGG CGGCGGGGAA GAAGTGGTCG GGTCGGTGCT GTGG

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wmc(15g5.1) Plate reference : 15g5.1
WMC locus number : WMC Length of sequence : 151
Last updated : Jeu 9 nov 2000 at 14:26
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the first insert. Trimmed to Rsa1 at both ends of insert. (GT)36 73 to 144. No room for primers.
PCR Comments :



Length of sequence : 151  Consensus sequence is :

..

GTACCATGTT CTTCAATTTA ACCTACCGAC CTGTGTGTAT GAACTGCATT 
GAACTAAATT TAATTGAAAA AGGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTTCTGTA 
C

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wmc586(15g5.2) Plate reference : 15g5.2
WMC locus number : WMC586 Length of sequence : 293
Last updated : Mar 28 Nov 2000 at 14:32
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the second insert. Trimmed to insert at both ends. Bases 177 to 209 show homology to 17e12 bases 171 to 203 (33 in longest run, maxScore = 52). (GT)13 57 to 82, (GT)18 195 to 230, (GT)9 233 to 250. Reverse primer has multiple sites of homology with 17e12, though each with several mismatches, forward primer is unique.
PCR Comments : Polymorphic at 61C anneal.
Forward primer details :
cgTgTcggATAATcgAcTTgc at 26 length 21 bases, Tm 65
Reverse primer details :
gTTgTgAgTcgATgcTTggc at 113 length 20 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 107 bases.



Length of sequence : 293  Consensus sequence is :

..

TGTGTGTGTT GATGGTCTCC AATTGCGTGT CGGATAATCG ACTTGCAACT 
AGAGGGGTGT GTGTGTGTGT GTGTGTGTGT GTCTTTCTGT CAAGGAGGGT 
CCCCAGTTGC ATGCCAAGCA TCGACTCACA ACTAGGGGTG TGTGTGCTTT 
GTGGAGGACC CCCAGTTGCA AGTTGCCCAT CGACTCGCAA CTAGGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT CTGTGTGTGT GTGTGTGTGT 
TTATTCATGT CCCCTAGTTG CAAGTTGACC ATCAACTCGT AAC

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wmc(15h5.1) Plate reference : 15h5.1
WMC locus number : WMC Length of sequence : 417
Last updated : Jeu 9 nov 2000 at 14:11
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the first insert. Trimmed to insert at both ends. Bases 16 to 40 show homology to 25f1 bases 45 to 69 (25 in longest run, maxScore = 23). No substantial microsatellite, multiple small microsatellites, though with not enough room for primers.
PCR Comments :



Length of sequence : 417  Consensus sequence is :

..

GAGAGAGAGA TGGACTCTTA GTTTGTGTGT GTGTGTGAGA AAGAGAGAGA 
GAGAGATGGA TTCTTAGTGT GTGTGGGGGT GTGTGCGTGC GTGTGTGTGT 
GTGTTTGTGT GTGTGCGTGT GAGAGAGAGA GAGATGGAGT CTTAGTGTGG 
TAGTTTTGGC CGATTCTGAC CCGTTTCGTG GACTTTTACT CACCGTTTTG 
GGGTCCCAAA GTGATTTCCA TTCTTGTTGA ACCCCAAGGT GCGCTTACGT 
GTCGGTCATC AACAGTCATA GTTTTGGCCG ATTCTGGCCC GTTTCGTGGA 
CTATTACTCA CTGTTTTGGG GTCCCGGAGT GATGTCCACG AGTAACGAAC 
CCTGTGGTGC ATTTACGTGT CGGTCATCAA CACTCGTAGT TTTGGCCGAT 
TCTGGCCCGT TTCGTGG

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wmc(15h5.2) Plate reference : 15h5.2
WMC locus number : WMC Length of sequence : 597
Last updated : Mar 28 Nov 2000 at 14:40
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Multiple inserts in this clone, this is the second insert. Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 340 to 462 (complement) show homology to 11b7 bases 35 to 157 (123 in longest run, maxScore = 165). (GGC)6 159 to 176. Close enough to classify as a duplicate of 11b7, 11b7 has priority.
PCR Comments :



Length of sequence : 597  Consensus sequence is :

..

CCAAGCAGCC AAGCAGGCGT ATCCACAACC GACGCGGGAG ATTATTCGGC 
CCGCCATGGC CACGCCAACG GAGAGGAGGA GAAAGGTGGC GTGTAAACTC 
ACAGGCATAC GCAGCACTGG AACCGCGGGT CCTCCAGGTC GTCGAAGCCA 
GCTCCACCGG CGGCGGCGGC GGCGGCCTCC TCCTTCTTCT CCGTCGGGTG 
GCTCGAGCTG CTGTCGTCGG AGGCCATCCT CCGCCTCGGT GGAAGTGGAA 
GGCGGATGGG GATTCTGCAG GTGCCGGGGG GGGTGAGCAG CCAAGATGCG 
ATGACTATGC GAGTGCCGAG TGCGCGAGAA AAGGGCGGCG AGGAAGGGGC 
CGAGCCGCAC CCCGCGGTTT GGGCCTTGGG GACGAAGGAA GGAACTGACC 
ATTCACGCTC CGTGTCCGGT TCGTCCGGTC GCGTTCGGGT GCCGTGTTTT 
AGTGCGTCCA GTTCACCCAC AAAATTGTCT CAAAAGAAAA AAGTTCACCC 
ACAAAACCAT ATCCTTCCCA GTTTGTATTG TTTTTTTTAA CATAATCCTT 
CACGATTTGG ATCGCGTGTC ATCGAGAGAG TTCGACGGAG ACGGTAC

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wmc(15a6) Plate reference : 15a6
WMC locus number : WMC Length of sequence : 542
Last updated : Jeu 9 nov 2000 at 14:26
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to Rsa1 at both ends of insert. (GT)8 18 to 33. Too difficult to make primers.
PCR Comments :



Length of sequence : 542  Consensus sequence is :

..

GTACTCTCTC TCTCCCTGTG TGTGTGTGTG TGTGCGTGTG TGTGTGTTCC 
CATTGTTTCC TGACTTGTGT TTTCCTGTCC AGATGGTGAA TATGCCAACC 
TTCTTTCAAA TCGAAGGGGG ACTGGTTATC AGGTCCAACC AATGGATGCA 
GTCTATGCCC CTTACTTGCA GGCAAACCCC AACTCCCCCC TTGGTGCAGC 
TGGAAGCATG AGTCCTTTCC GGGGAAGTAA CTTTTCTGGT TCAGGCCACA 
TGGATAATGC TGCATATCAA AAGGCTTATC TTGCATCATT ACTTGCTCAG 
CAGAAGCTAC AGTATGGAAT GCCATACATG GGCAAATCTG GTGGTCTTAG 
CCCAACTCTT TATGGCAGTG AACAAGCATA TGGCATGGAC ATGGCATATT 
TATCAAGTCC AACATCTAGC CATTTTATCC CATCTCCCCA GGGTCATGCC 
AGGCAAGGGG ATAGGCTAAC AACACGTATT CCATCCATGG CAAGGAGCAC 
CACTGGAGGA GCTATGGGAT CATGGAGTTC AGAGAATGGT AC

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wmc(15b6) Plate reference : 15b6
WMC locus number : WMC Length of sequence : 275
Last updated : Mar 28 Nov 2000 at 15:04
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 1 to 50 show homology to 9d1 bases 11 to 60 (50 in longest run, maxScore = 116). (GT)21 163 to 204.15b6 is a duplicate of 9d1. 9d1 has priority.
PCR Comments :



Length of sequence : 275  Consensus sequence is :

..

CTATTTTAAG TGAATCCTTA TTATAACATG CACACAAATT TTGGGCACTT 
TTATTCGACT TAAGTCAGTG TGCCACCGTA TCTAGTATAT TTACTACTCC 
CTCCGTCTAG GTGTAATAAG TCACATTAGA AGGTGCAACG GGACCAAGGT 
GCGTGCGTGT GCGTGTGTGT GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT 
GTGTTTAACA ACATGTGTGC GTTAGAGAGA GCGACATATC GACCTACTCC 
TATAGAGAGA TGGTGTGTAT TGTAC

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wmc(15c6) Plate reference : 15c6
WMC locus number : WMC Length of sequence : 243
Last updated : Jeu 9 nov 2000 at 14:27
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 135 to 243 (complement) show homology to 8a11 bases 1 to 109 (109 in longest run, maxScore = 150). (CA)10 42 to 61. Too difficult to make primers.
PCR Comments :



Length of sequence : 243  Consensus sequence is :

..

CCCCCCACAC ACACACACTA AGACTCCATC TCTCTCTCTC TCACACACAC 
ACACACACAC ATACACACAC ACATCACACA CACACTAAGA CTCCATCTCA 
CACACACATG CTCTGGGCGG AGCATTTGTT CCTGCCACCC TTCATCCAAC 
CTTACCCATA TGATAAACAA TCACCTTTAT TTACTTGCAT AACATGGACA 
AATTCCACTT TACTTTAGTA GTCAGCAAAC TTATCATCTG TAC

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wmc587(15d6) Plate reference : 15d6
WMC locus number : WMC587 Length of sequence : 273
Last updated : Mar 28 Nov 2000 at 15:28
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to insert at both ends. (GT)26 79 to 130.
PCR Comments : No amplification at 61C anneal. Monomorphic several bands at 51 C anneal.
Forward primer details :
gAgcTcgcAgTTTgcTgAcA at 9 length 20 bases, Tm 65
Reverse primer details :
AcgTgcAAgTgcAcgTAAgg at 217 length 20 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 228 bases.



Length of sequence : 273  Consensus sequence is :

..

GTACCTACGA GCTCGCAGTT TGCTGACATA TTCACTAAGC TCAACGTCGG 
GGGCACCACG TTTCAGCTGG GGGGGGGGGT GTGTGTGTGT GTGTGTGTGT 
GTGTGTGTGT GTGTGTGTGT GTGTGTGTGT TGGAATATGC CCACGCATGC 
TACTACTCCT GGCTAACTCC CCACGACCGG ATAGGTCGGG GCTCAACTAC 
ACGCGCACTC CGTGCGCCTT ACGTGCACTT GCACGTCCTG CATGCAAGGG 
ATAACCCAGC CATGCCGTTG TAC

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wmc(15e6) Plate reference : 15e6
WMC locus number : WMC Length of sequence : 269
Last updated : Jeu 9 nov 2000 at 14:11
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 1 to 269 show homology to 12c5 bases 1 to 269 (269 in longest run, maxScore = 222). No substantial microsatellite.
PCR Comments :



Length of sequence : 269  Consensus sequence is :

..

GCGGAGCAGG TGCTCTATAG CCTTCTTCGA GATGACAATG CCGAAGAGGT 
CGAGCTTCTT GAGTTGAGGA AGAAGAAGGG CGGGCGCGGC ATTAATCTGG 
GGAAGATAGT AGGAGCTGAA GCTGGCGCGG CGCAGCGTTG GCGTGAGGCG 
GAGTGCGGAC GGCGGCAGAG AGCGACATCG TCCAGCTTCA AAGCTGAGCT 
CCTCGAGCTG ATCTAGGGCG GCGGATAAGA ACCACTCGTC GAACTTGGGT 
TGGACCTTGC AGTTGGTAC

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wmc588(15f6) Plate reference : 15f6
WMC locus number : WMC588 Length of sequence : 295
Last updated : Mar 28 Nov 2000 at 15:36
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to Rsa1 at 5' end and to insert at 3' end. Bases 145 to 163 (complement) show homology to 2c12 bases 227 to 245 (19 in longest run, maxScore = 77). (GT)13 75 to 100. Reverse primer has 5 mismatches to 2c12, forward primer is unique.
PCR Comments : Polymorphic at 61C anneal.
Forward primer details :
agtgagatgTcAgAccTAccTATTTgg at 5 length 27 bases, Tm 64
Reverse primer details :
gcgTgcgAcTcATcTgcTATT at 272 length 21 bases, Tm 64
Expected size of amplified fragment (Chinese Spring=) 288 bases.



Length of sequence : 295  Consensus sequence is :

..

GTACAGTGAG ATGTCAGACC TACCTATTTG GGGGTATGTG TTCATAGCTA 
GGATTCCCCT CCCGGCAACA TGAAGTGTGT GTGTGTGTGT GTGTGTGTGT 
TTGGCGGGGG GGGCTAGCAA TAATAACACG CGCTTTGTGC GACCGGTGCC 
ACATCAAAGT TGTGCATTGG GTGTTTGAAC ATCAAACCAC CCTTTTCATA 
CATATACTTG GTCCGCATGC AAGTATGTTC GTGTTCTGAC CCTTTCCCAC 
ATTTTTCTGA CCAAATTTAT AAATAGCAGA TGAGTCGCAC GCAGC

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wmc(15g6) Plate reference : 15g6
WMC locus number : WMC Length of sequence : 216
Last updated : Jeu 9 nov 2000 at 14:27
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : Trimmed to insert at both ends. (CA)14 9 to 36. No room for primers.
PCR Comments :



Length of sequence : 216  Consensus sequence is :

..

GTACCAAACA CACACACACA CACACACACA CACACAGAGA GAGAGAAGAT 
GAGCTCCATA TTAGTCAATT TTGCCGCTAC CACGTTGATT TGTATCTGTG 
CAACCATCTC CATGTTGTTT GTATTGTTTG GATTGCAAGA AATACAGTTT 
TGGGTGACCT ACACTTAGTT TAACTTCTCT AAAAAATCAA CCATTTTACA 
TAAATATGTT GTGTAC

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wmc(15h6) Plate reference : 15h6
WMC locus number : WMC Length of sequence : 6
Last updated : Jeu 9 nov 2000 at 12:22
Owner : Aventis Date at Agrogene : 27.10.00 Public Domain : Yes
Comments : No sequence 27.10.00.
PCR Comments :
No sequence available for this clone
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