|
Development of new STMS markers for sugarcane Govindaraj P. , Balasundaram N. , Sharma T.R. , Bansal K.C. , Koundal K.R. and Singh N.K. |
Sugarcane Breeding Institute, Coimbatore, Tamilnadu, India.
NRC on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, India.
|
Marker Name |
Source sequence |
EST/ Genomic |
SSR motif |
Forward primer (5'- 3') |
Reverse primer (5' - 3') |
Ta (Deg C) |
No of bands |
Product size range |
|
AA577668 |
EST |
(gaa)6 |
tggcatgtgtcatagccaat |
ccccaactgggacttttaca |
58 |
3 |
207 - 228 |
|
|
AA961302 |
EST |
(ga)13 |
gctgtcccgttccaagttac |
gcgaccggattatgatgatt |
58 |
4 |
185 - 216 |
|
|
AF062734 |
EST |
(tgc)5 |
cgtgttcctcttcaacaacg |
tgcttcgctatatatgggttca |
58 |
2 |
191,198 |
|
|
SHY293476 |
Genomic |
(gt)28 |
atagctcccacaccaaatgc |
ttggcaaaattgacccaaat |
54 |
5 |
148 - 182 |
|
|
SHY293477 |
Genomic |
(tg)32 |
tccaaattgcctgttgttttc |
cttacacatgcacaggcaca |
58 |
6 |
158 - 194 |
|
|
SHY293478 |
Genomic |
(cgg)9 |
ttacagcctggagctcgttt |
cgaagcctctcctctcctc |
58 |
3 |
174 - 186 |
|
|
SHY293479 |
Genomic |
(cgg)6 |
gtgacagcggcttgttcag |
ttaaacacgcagccattcag |
58 |
4 |
185 - 256 |
|
|
SHY293481 |
Genomic |
(cgc)6 |
ctttcagtggccatctccat |
gaatgcgcagggataggata |
58 |
4 |
147 - 162 |
|
|
SHY293483 |
Genomic |
(ct)17 |
caccactcacatccacttgc |
tatggagagatgctgctgct |
64 |
7 |
130 - 224 |
|
|
SHY293484 |
Genomic |
(ag)23 |
cagccacgtgatgctttct |
ccgatccatcagtttcaggt |
58 |
5 |
134 - 164 |
|
|
SHY293487 |
Genomic |
(ga)22 |
ttccaccagtgacattcagc |
ccaacagcagcttcttcctt |
58 |
6 |
172 - 206 |
|
|
SHY293488 |
Genomic |
(ag)19 |
aacccattgaccagatccag |
tagtggccctaggcgtaaaa |
58 |
4 |
155 - 183 |
|
|
SHY293489 |
Genomic |
(ag)23 |
gacagaatatgccatggataacaa |
cgttctctggtcctattgagc |
58 |
4 |
187 - 210 |
|
|
SHY293490 |
Genomic |
(ag)24 |
gctcgccatgaatagaaagg |
accgaggtaggagggagtgt |
64 |
3(Gel) |
207 - 226 |
|
|
SHY293494 |
Genomic |
(ga)18 |
cagccaagggtgagaaaaag |
ttttactatgcaccaagatacacg |
56 |
6 |
136 - 350 |
|
|
SHY293495 |
Genomic |
(ga)20 |
taagccattgggaagaggtg |
ctgatgcctgggaatctttc |
58 |
5 |
168 - 191 |
|
|
SHY293497 |
Genomic |
(ga)17 |
gctaagttgccggatgagaa |
gtgatggcgtgaacaatgac |
49 |
8(Gel) |
169 - 343 |
|
|
SHY293498 |
Genomic |
(ga)18 |
taaacccccgaaaaagaacc |
tccggaggtagatccatttg |
56 |
9(Gel) |
113 - 498 |
|
|
SHY293499 |
Genomic |
(ga)34 |
tatatggcgaggacagatgc |
gggttcagaattagagcaatcg |
64 |
7 |
141 - 183 |
|
|
SHY293500 |
Genomic |
(ag)27 |
tccatgcatgcgtgtagttt |
agtgcacaacgttcttgctg |
58 |
5(Gel) |
163 - 198 |
|
|
SHY293501 |
Genomic |
(tg)18 |
gttctcgacatgggcctact |
ctgcactttcggtccttttt |
54 |
7(Gel) |
194 - 264 |
|
|
SHY293502 |
Genomic |
(ga)20 |
tggatttgggtaaggatgga |
taatgcctctgggctcaaat |
58 |
7 |
184 - 260 |
|
|
SHY293503 |
Genomic |
(ag)27 |
gtgctgggattctgagcttc |
gcaagttcttggcctttgtt |
58 |
11 |
81 - 194 |
|
|
SHY293505 |
Genomic |
(ga)20 |
tagtggtccaaaagcccaag |
cggttagcgcactctctctc |
59 |
5 |
145 - 174 |
|
|
SHY293561 |
Genomic |
(cgg)7 |
ctccttctccttcgcatcct |
cacctttctggagcacgtta |
58 |
4 |
183 - 305 |
|
|
SHY293562 |
Genomic |
(cgg)8 |
aaccaccactcatcgtcctc |
caccgagttcccattgttct |
58 |
4 |
191 - 234 |
|
|
SHY293563 |
Genomic |
(tc)36 |
ccaactcactcaccccagtt |
atgagagtgcagatgcatgg |
52 |
5 |
160 - 190 |
|
|
SHY293564 |
Genomic |
(tgt)6 |
acaggagcgcttggagatta |
gagcagaagggctagaagca |
57 |
3 |
111 - 178 |
|
|
SHY293573 |
Genomic |
(gt)18 (ga)31 |
cgtcttgtggattggattgg |
tggattgctcaggtgtttca |
58 |
9 |
131 - 214 |
|
|
SHY401316 |
Genomic |
(ag)15 |
tgaactcggcaacagttttt |
cccaccaagtcgttctgaat |
55 |
9(Gel) |
92 - 292 |
|
|
SHY401318 |
Genomic |
(tg)36 |
gatggaggctttgcaatgat |
gcatgtcccactgaactgaa |
55 |
7 |
204 - 335 |
|
|
SHY401320 |
Genomic |
(tg)35 |
accgattgttcagtgggaag |
aacctagcaatttacaagagaattaga |
57 |
4 |
138 - 158 |
|
|
SHY401321 |
Genomic |
(tg)20 |
ctgatgggaggttgaaggaa |
ataagcaccaaaagcgtggt |
57 |
4 |
143 - 508 |
|
|
SHY401325 |
Genomic |
(tg)35 |
gtcggtcgtgagaaggaaag |
cacgtataaaggccctgtgg |
58 |
5 |
132 - 155 |
|
|
SHY401326 |
Genomic |
(tg)24 |
acaataaccccgcagacatc |
taatgcgtcatttggagcag |
58 |
3 |
182 - 203 |
|
|
SHY401328 |
Genomic |
(tg)27 |
ggaagcaagcaagaagaacc |
tctctgcagtgtgtgtgctg |
55 |
5 |
135 - 204 |
|
|
SHY401330 |
Genomic |
(tg)19 |
ctcacgtcctgttggtgcta |
tacatgggacacatgcttgc |
55 |
5 |
115 - 161 |
|
|
SHY401331 |
Genomic |
(tg)41 |
cgaaggaccagttgaaagga |
atggaacaggacacaccaca |
58 |
8 |
119 - 206 |
|
|
SHY401332 |
Genomic |
(tg)31 |
tttggagagcaaggagcaat |
ccaccgtatgcatgaaagaa |
53 |
6 |
136 - 230 |
|
|
SHY401333 |
Genomic |
(gt)24 |
ggcctatggaacgaagttca |
cagccttttcttcgcaaaac |
58 |
5 |
176 - 250 |
|
|
SHY401334 |
Genomic |
(gt)28 |
agctcacgtgtgtgtgtgtg |
gtgcagtgtcaggggaccta |
58 |
6 |
126 - 177 |
|
|
SHY401337 |
Genomic |
(tg)19 |
ctatacggcaaacgcaacct |
tatacgtcgcatgcaccatc |
58 |
6 |
155 - 201 |
|
|
SHY401338 |
Genomic |
(gt)27 |
cgagcctccctccatagatt |
accaccaccaacctcatctc |
55 |
3 |
185 - 223 |
|
|
SHY401340 |
Genomic |
(tg)23 |
gttagcattgctgggaggag |
tctagctgggcaattccaaa |
53 |
2 |
128 , 147 |
|
|
SHY401342 |
Genomic |
(gt)16 |
ttggacatggcaagtctttg |
aggaacctcccaagaacaca |
55 |
9 |
114 - 218 |
