QTL scans and thresholds from a sCIM analysis with MQTL for winterhardiness
The simplified Composite Interval Mapping (sCIM) approach available in the MQTL software package (Tinker and Mather, 1995) was used for QTL detection in conjunction with the skeleton linkage map. Each data set was analyzed with 1,000 permutations, a 5 cM walk speed, and a Type I error rate of 5%. Eighteen background markers were used, and these markers were spaced evenly across the seven linkage groups. QTL analyses were conducted for eleven phenotypes. QTL for nine of these phenotypes - heading date at 8, 16, and 24 h with and without vernalization; field heading date; and Montana and Oregon field survival - were described by Pan et al., (1994) based on a MAPMAKER/QTL analysis and a 78-point map. QTL for crown fructan content and LT50 (temperature lethal to 50% of a test population) were described by Hayes et al. (1992) using a 33-point map and QTL-STAT. Thus, this report describes QTL for previously-reported phenotypes using a more complete linkage map and contemporary QTL detection procedures.
Hayes PM, Chen THH, Blake TK (1992). Marker-assisted genetic analysis of cold tolerance in winter barley. In: PH Li and L Christersson eds, Advances in Plant Cold Hardiness. CRC Press, Boca Raton, USA
Pan A, Hayes PM, Chen F, Chen THH, Blake T, Wright S, Karsai I, and Bedo Z (1994). Genetic analysis of the components of winterhardiness in barley (Hordeum vulgare L.). Theor Appl Genet 89:900-910
Tinker N, and Mather D (1995) Methods for QTL analysis with progeny replicated in multiple environments. JQTL: (http://probe.nalusda.gov:8000/otherdocs/jqtl)