The Dicktoo x Morex Barley Mapping Population

K. Meszaros, Martonvasar Research Institute of the Hungarian Academy of Sciences, POB 19, H2462, Martonvasar, Hungary, klara@fsnew.mgki.hu

P. Hayes, Dept. of Crop and Soil Science, Oregon State University, Corvallis, OR USA hayesp@css.orst.edu

A lot of folks have worked with the Dicktoo x Morex population over the years, and there is still a lot to be learned! Our thanks to the many cooperators who appear throughout the DxM publication list. If we can be of any assistance in supplying you with seed, data, or addresses of cooperators, don't hesitate to contact us.

Winterhardiness in cereals is the final expression of a number of interacting component traits, including, but not limited to, low temperature tolerance, growth habit, photoperiod response, and crown fructan content (Hayes et al., 1996). Responses to selection for winterhardiness have been limited, leading Fowler and Gusta (1979) to conclude that genetic variation may be exhausted. Thomashow (1990) summarized over 20 reports that, cumulatively, assign cold tolerance genes to every chromosome in each of the three genomes of wheat. Based on homoeology, this would mean a cold tolerance gene on each of the seven chromosomes of barley. Given this combination of complexity and poor prospects, progress in developing superior winter habit barley varieties (and in understanding the genetic mechanisms determining winterhardiness) may best be achieved by locating the genetic determinants of each component trait. Quantitative trait locus (QTL) analysis is a useful tool for dissecting complex phenotypes. Like Becker et al. (1995), we were interested in determining the utility of AFLP based markers for linkage map construction in barley. Our longer-term goal was using the map for detection of winterhardiness related QTL. Our model was the Dicktoo x Morex population. This doubled haploid (DH) population is the subject of extensive collaborative mapping of winterhardiness-related traits, including low temperature tolerance under field and controlled environment conditions, photoperiod response, vernalization response and maturity. All previous reports were based on a map that provided partial genome coverage (Blake et al. 1993; Chen et al. 1996; Hayes et al. 1992; Hayes et al. 1993; Hayes et al. 1996; Oziel et al. 1996; Pan et al. 1994; van Zee et al. 1995). The objective of this investigation was to use an enhanced, complete genome map and a new tool for QTL detection to fully characterize the Dicktoo x Morex barley population for winterhardiness-related traits.

This page provides access to:

Winterhardiness-related phenotypes

A base map and genotype data used to construct that map

QTL scans and thresholds from a sCIM analysis with MQTL for winterhardiness

Detailed analysis of a subset of lines

A continuously updated list of publications based on Dicktoo x Morex data

Seed of the Dicktoo/Morex doubled haploid germplasm, including parental stocks, may be obtained from Patrick Hayes, Dept. of Crop and Soil Science, Oregon State University, Corvallis, OR 97331. hayesp@css.orst.edu

REFERENCES

Becker B, Vos P, Kuiper M, Salamini F, and Heun M (1995) Combined mapping of AFLP and RFLP markers in barley. Mol Gen Genet 249:65-73.

Blake T, Tragoonrung S, Walton, M, Wright S, Jones, B, Chen, T, and Hayes P (1993) Mapping the genes for cold tolerance in barley. In: TJ Close, EA Bray, eds, Responses of Plants to Cellular Dehydration During Environmental Stress. American Society of Plant Physiologists, Rockville, MD, pp 202-210

Chen THH, Hayes PM, Pan A, Chen FQ, vanZee K, Blake, TK, Karsai I, Close TJ (1996) Marker-assisted genetic analysis of winter hardiness in barley. In: Nover L, and A Leone eds, Genes and their products for tolerance to physical stresses in plants. European Sci Foundation (in press)

Fowler DB, and Gust LV (1979) Selection for winterhardiness in wheat: I. Identification of genetic variability Crop Sci 19:769-772

Hayes PM, Chen THH, Blake TK (1992). Marker-assisted genetic analysis of cold tolerance in winter barley. In: PH Li and L Christersson eds, Advances in Plant Cold Hardiness. CRC Press, Boca Raton, USA

Hayes PM, Blake T, Chen, THH, Tragoonrung S, Chen F, Pan A, and Liu B (1993) Quantitative trait loci on barley (Hordeum vulgare) chromosome 7 associated with components of winterhardiness. Genome 36:66-71.

Hayes PM, Chen FQ, Kleinhofs A, Kilian A, and Mather D (1996) Barley genome mapping and its applications In: PP Jauhar ed, Methods of Genome Analysis in Plants. CRC Press, Boca Raton, USA

Oziel A, Hayes PM, Chen FQ, and Jones B (1996) Application of quantitative trait locus mapping to the development of winter-habit malting barley. Plant Breeding 115:43-51

Pan A, Hayes PM, Chen F, Chen THH, Blake T, Wright S, Karsai I, and Bedo Z (1994). Genetic analysis of the components of winterhardiness in barley (Hordeum vulgare L.). Theor Appl Genet 89:900-910

Thomashow MF (1990) Molecular genetics of cold acclimation in higher plants. Adv Genet 28:99-131

van Zee K, Chen FQ, Hayes PM, Close TJ, and Chen THH (1995) Cold-specific induction of a dehydrin gene family member in barley. Plant Physiol 108:1233-1239

Thanks to Lois Gangle and Ann Corey for getting this document off the ground on onto the Net.