TABLE 1
Integration of 35 translocation breakpoints of chromosome 1H (5) into the Igri/Franka-derived RFLP linkage map
|
Genetic map |
Physical map |
||||
|
TBb |
Position (mGN) |
Position (FL) |
|||
|
Markera |
Position (cM) |
Obtained by karyotyping |
Refined by PCR-mappingc |
||
|
MWG2021.a |
4.4 |
||||
|
Ris45.b |
8.8 |
||||
|
MWG896 |
20.3 |
||||
|
T54ai |
S 38-50 |
S 38-50 |
S 0.88 |
||
|
T56ap |
S 10-46 |
S 31-43 |
S 0.74 |
||
|
ABG500.a |
30.3 |
||||
|
MWG913 |
34.6 |
||||
|
T57ac |
S 24-50 |
S 31-43 |
S 0.74 |
||
|
MWG2056 |
36.0 |
||||
|
T51ak |
S 31-50 |
S 31-35 |
S 0.66 |
||
|
T57aj |
S 19-40 |
S 23-40 |
S 0.63 |
||
|
T56x |
S 10-32 |
S 23-32 |
S 0.55 |
||
|
T56ab |
S 10-50 |
S 23-28 |
S 0.51 |
||
|
T51am |
S 23-32 |
S 23-27 |
S 0.50 |
||
|
cMWG758 d |
37.4 |
||||
|
T57ab |
S 23 |
S 23 |
S 0.46 |
||
|
T53ae |
S 10-40 |
S 10-26 |
S 0.36 |
||
|
T57ad |
S 11-17 |
S 11-17 |
S 0.28 |
||
|
T56aj |
S 10-12 |
S 10-12 |
S 0.22 |
||
|
T54aq |
S 10-11 |
S 10-11 |
S 0.21 |
||
|
T51a |
0 |
0 |
0 |
||
|
CENTROMERE |
(41.0) |
(0) |
|||
|
T52ak |
L 9-24 |
L 9-24 |
L 0.23 |
||
|
T53af |
L 9-24 |
L 9-24 |
L 0.23 |
||
|
T56aa |
L 9-24 |
L 9-24 |
L 0.23 |
||
|
T57ap |
L 9-24 |
L 9-24 |
L 0.23 |
||
|
T51v |
L 9-24 |
L 9-24 |
L 0.23 |
||
|
T52af |
L 12-22 |
L 12-22 |
L 0.24 |
||
|
T54ah |
L 11-24 |
L 11-24 |
L 0.24 |
||
|
T54e |
L 9-24 |
L 15-24 |
L 0.27 |
||
|
T54ac |
L 9-24 |
L 22-24 |
L 0.32 |
||
|
T54ap |
L 13-24 |
L 22-24 |
L 0.32 |
||
|
T56ah |
L 29-48 |
L 29-31 |
L 0.42 |
||
|
T54ad |
L 29-41 |
L 29-35 |
L 0.44 |
||
|
T54af |
L 29-62 |
L 29-35 |
L 0.44 |
||
|
T57ao |
L 29-62 |
L 29-35 |
L 0.44 |
||
|
MWG800 d |
44.6 |
||||
|
T57ag |
L 33-49 |
L 33-35 |
L 0.47 |
||
|
MWG506 d |
50.5 |
||||
|
Ris13 d |
50.5 |
||||
|
MWG2217 |
61.0 |
||||
|
T56z |
L 29-59 |
L 44-59 |
L 0.72 |
||
|
MWG943 |
61.0 |
||||
|
MWG2077 |
63.9 |
||||
|
T52ad |
L 42-72 |
L 53-62 |
L 0.80 |
||
|
cMWG649b |
80.3 |
||||
|
T57af |
L 29-62 |
L 53-62 |
L 0.80 |
||
|
cMWG706a |
86.6 |
||||
|
cMWG676 |
86.6 |
||||
|
MWG733 |
101.0 |
||||
|
MWG954 |
102.4 |
||||
|
MWG984.b |
108.2 |
||||
|
T52as |
L 46-72 |
L 57-66 |
L 0.86 |
||
|
MWG2028 |
111.2 |
||||
|
cMWG701b |
112.7 |
||||
|
T52ah |
L 66-72 |
L 66-67 |
L 0.92 |
||
|
MWG632.b |
118.3 |
||||
|
MWG912 |
129.7 |
||||
|
T57an |
L 29-72 |
L 66-67 |
L 0.92 |
||
|
ABG387a |
132.6 |
||||
a
Markers representing reference points to the NABGMP Steptoe/Morex map (Graner et al. 1993) are shown in bold. Single-copy markers according to Andreas Graner (personal communication) are underlinedb
Translocation breakpoints (TBs) used for defining chromosomal subregions for comparison of the physical and genetic maps are marked in boldc
The refined TB positions are based on a final analysis considering 240 genetically mapped TBs of 120 T-linesd
Arm-specificity of STS derivatives confirmed by PCR with DNA of microisolated telosomes of barley telotrisomic stocks (Singh and Tsuchiya 1982)e
Arm-specificity of STS derivatives confirmed by PCR with DNA of barley-wheat telosome addition lines (Islam et al. 1983), based on amplified polymorphic fragments between wheat (cv. Chinese Spring) and barley (cv. Betzes)