TABLE 1

Integration of 35 translocation breakpoints of chromosome 1H (5) into the Igri/Franka-derived RFLP linkage map


 
Genetic map 
Physical map 
    TBb
Position (mGN) 
Markera Position (cM)   Obtained by karyotyping Refined by PCR-mappingc
MWG2021.a 4.4       
Ris45.b 8.8       
MWG896 20.3       
    T54ai  S 38-50 S 38-50
    T56ap S 10-46 S 32-43
ABG500.a 30.3       
MWG913 34.6       
MWG2056 36.0       
    T51ak  S 31-50 S 31-32
    T57ac  S 24-50 S 24-43
    T57aj  S 19-40 S 24-40
    T56x  S 10-32 S 24-32
    T56ab  S 10-50 S 24-26
    T51am  S 23-32 S 23-24
cMWG758 d 37.4       
    T57ab S 23 S 23
    T53ae  S 10-40 S 10-13
    T57ad  S 11-17 S 11-17
    T56aj  S 10-12 S 10-12
    T54aq  S 10-11 S 10-11
    T51a  0 0
CENTROMERE (41.0)   (0)  
    T57ap  L 9-24 L 9-24
    T56aa  L 9-24 L 9-24
    T53af  L 9-24 L 9-24
    T51v  L 9-24 L 9-24
    T54ah L 11-24 L 11-24
    T52ak L 9-24 L 18-19
    T54e  L 9-24 L 15-23
    T52af  L 12-22 L 21-22
    T54ap L 13-24 L 24
    T54ac  L 9-24 L 24
    T56ah L 29-48 L 29-30
    T54ad L 29-41 L 29-34
    T54af  L 29-62 L 29-34
    T57ao L 29-62 L 29-34
MWG800 d 44.6       
    T57ag  L 33-49 L 33-34
MWG506 d 50.5       
Ris13 d 50.5       
MWG2217 61.0       
    T56z  L 29-59 L 45-59
MWG943 61.0       
MWG2077 63.9       
    T52ad L 42-72 L 55-62
cMWG649b 80.3       
    T57af L 29-62 L 56-62
cMWG706a 86.6       
cMWG676 86.6       
MWG733 101.0       
MWG954 102.4       
MWG984.b 108.2      
    T52as L 46-72 L 58-66
MWG2028 111.2       
cMWG701b 112.7       
    T52ah  L 66-72 L 66-67
MWG632.b 118.3       
MWG912 129.7       
    T57an L 29-72 L 66-67
ABG387a 132.6       
a Markers representing reference points to the NABGMP Steptoe/Morex map (Graner et al. 1993) are shown in bold. Single-copy markers according to Andreas Graner (personal communication) are underlined

b Translocation breakpoints (TBs) used for defining chromosomal subregions for comparison of the physical and genetic maps are marked in bold

c The refined TB positions are based on a final analysis considering 240 genetically mapped TBs of 120 T-lines

d Arm-specificity of STS derivatives confirmed by PCR with DNA of microisolated telosomes of barley telotrisomic stocks (Singh and Tsuchiya 1982)

e Arm-specificity of STS derivatives confirmed by PCR with DNA of barley-wheat telosome addition lines (Islam et al. 1983), based on amplified polymorphic fragments between wheat (cv. Chinese Spring) and barley (cv. Betzes)