The Oregon Wolfe Barleys: Latest Howling Successes

Serena McCoy (1), Ann Corey (1), Jose Costa (2), Patrick Hayes (1), Tanya Filichkin (1), Claudio Jobet (3) Carl Jones (4), Andris Kleinhofs (5), Oscar Riera-Lizarazu (1), Jennifer Petersen (3), Kazuhiro Sato (6), Peter Szucs (7), Theerayut Toojinda (8), Isabel Vales (1)
Dept. of Crop and Soil Science
Oregon State University
Corvallis, OR 97331, USA
(2) Dept. of Natural Resource Science and Landscape Architecture
University of Maryland
College Park, MD, USA
(3) INIA/Carillanca
Temuco, Chile
(4) Dept. of Horticulture
Oregon State University
Corvallis, OR 97331, USA
(5) Dept. of Agronomy and Soils
Washington State University
Pullman, WA 99164, USA
(6) Research Institute for Bioresouces
Okayama University
Kurashiki 710, Japan
(7) Martonvasar Research Institute
H2462, Martonvasar, Hungary
(8) National Center for Genetic Engineering and Biotechnology
Kasetsart University
Nakorn Pathom 73140, Thailand.

The Oregon Wolfe Baley's (OWBs) are a tool for barley genetics research and instruction. The population of 94 doubled haploid lines was developed from the F1 of a cross between Dr. R. Wolfe's dominant and recessive marker stocks. The phenotypes resulting from segregation and independent assortment of alleles at 13 easily-scored morphological loci are visually impressive. These morphological loci are now positioned on a comprehensive linkage map built with an array of molecular markers. Due to its high level of polymorphism and integration of molecular and morphological marker loci, the OWB population will be a useful mapping resource. At the same time, the population can be used as a plant genetics and breeding teaching tool. For example, the epistatic interaction between the hooded (K) and short awn (lk-2) loci does not allow mapping of the k locus based on phenotypic data. In individuals that are homozygous for the recessive allele of lk-2, the expression of the hooded phenotype is masked, resulting in the expression of a short-awned, rather than hooded, phenotype. With graphical genotype analysis, we predicted the K locus genotypes of short-awned individuals based on their genotypes for flanking DNA markers. The predicted genotypes were then confirmed by mapping a barley homeobox gene (hvknox3) believed to represent the hooded (K) locus. The hvknox3 locus mapped to BIN 4 of barley chromosome 4 (4H), the map position of the K locus. Please visit our website: http://www.css.orst.edu/barley/WOLFEBAR/WOLFNEW.HTM.