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GrainGenes Trait Study Report: beta-Esterase activity, Souza88

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Trait Study	beta-Esterase activity, Souza88
Trait	beta-esterase activity
Reference	Souza E (1988) Measures and applications of genetic relationships in oats.
Protocol	Alpha and Beta-esterases were distinguished in separate gel slices by their relative specificity for alpha-napthyl acetate, and beta-napthyl acetate. The stain for beta-esterase activity consistently produced a single zone of activity in each lane at either Rf 0.45 or Rf 0.51. Low levels of cross reactions with the alpha esterase isozymes were observed. The enzymes controlled by Be-1, however, show much greater activity with beta-napthyl acetate as a substrate than with alpha-napthyl acetate. The fast migrating form of the enzyme occured in the cultivars TAM O-312 CIav9199 and Astro while the more common slow allozyme occured in the cultivars Salem and Ogle. The crosses Ogle/Astro and Astro/Salem had a codominant segregation pattern fitting a 1:2:1 ratio, indicating that these two zones are alternate allozymes of a single locus designated Be-1 with the alternate alleles BE1`0.51` and BE`0.45`. Additionally, the cultivar Sierra was observed to have beta-esterase activity at Rf 0.38 but not Rf 0.51 or 0.45. This appears to be an alternative form to the BE1`0.51` and BE1`0.45` alleles. No crosses were made to confrim this hypothesis.
Values	0.38 = BE-1`0.38` 0.45 = BE-1`0.45` 0.51 = BE-1`0.51`
Germplasm Description	Advance (oat) 0.45   BE-1`0.45` [ Show all 70 ]

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