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GrainGenes Sequence Report: CB878820

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Sequence
CB878820
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC168864
NCBI UniGene
Hv.22655
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'CDNA clone: FLbaf55b12, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
HP
Tissue
epidermis
Developmental Stage
seedlings grown in greenhouse for 6 days
Data Source
genbankRelease 136, Jun 15 2003
genbankUpdated Nov 2006
Title
HP09O16T HP Hordeum vulgare subsp. vulgare cDNA clone HP09O16 5-PRIME, mRNA sequence.
Strain
lab_host XL10-Gold
vulgare
Clone
HP09O16
Probe
GBS495
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Stein Nils; Molecular Markers Group, Department Genbank; Institute of Plant Genetics and Crop Plant Research (IPK); Corrensstr. 3, 06466, Gatersleben, Germany; Tel: 039482-5522; Fax: 039482-5595; Email: stein@ipk-gatersleben.de; Insert Length: 690 Std Error: 0.00; Plate: 9 row: O column: 16; Seq primer: T3.
Note: Vector: pBluescript SK+; Site_1: EcoRI (5'-end of cDNA); Site_2: XhoI (3'-end of cDNA); Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 850 bp
DNA
ggctgcaggcggcatcctcctctctacagttaatacagtaccatggcccc
cgccgtgatggcttcgtcggctaccaccgtcgcacccttccaggggctca
agtccacagccggtctcccggtcagccgccgctccagcgccagcctcggc
agcgtcagcaatggcggaaggatcaggtgcatgcaggtgtggccgattga
gggtatcaagaagttcgagaccctgtcttacttgccacccctcaccacgg
aggccctcctgaagcaggttgactacctgatccgctccaagtgggtgccc
tgcctcgagttcagcaaggttggcttcgtcttccgtgagcacaacagctc
ccccgggtactacgacggccgatactggacaatgtggaagctgcctatgt
ttgggtgcaccgacgctacacaggtgcttaacgaggtggaggaggtcaag
aaggagtaccctgacgcttacgtccgtatcatcggcttcgacaacctgcg
gcaggtgcagtgcgtcagcttcatcgccttcaggccaccgggttgcgagg
agtccggcaaggcataaactacgaggtgaaagaatggcctatgtcgtatg
aaggtcttttgtgaacttcacttttgtccacgactctgctttccgaccag
agcataatttcttctctatgatctgtgtatgtacgagcat

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