GrainGenes Sequence Report: AL508997
Sequence
AL508997
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC169689
NCBI UniGene
Hv.3227
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'ADP-glucose pyrophosphorylase small subunit'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Tissue
developing caryopsis (3.-15.DAP)
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
AL508997 Hordeum vulgare Barke developing caryopsis (3.-15.DAP) Hordeum vulgare subsp. vulgare cDNA clone HY10G16V 5', mRNA sequence.
Strain
lab_host XLOLR vulgare
Clone
HY10G16V
Probe
GBS835
Remark
DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Michalek W; Institute for Plant Genetics and Crop Plant Research; Corrensstr.3, D-06466 Gatersleben, Germany; Email: michalek@ipk-gatersleben.de, http: Note: Vector: plasmid pBK-CMV; Site_1: EcoRI; Site_2: XhoI; mRNA was made from developing caryopsis (3.-15.DAP) of spring barley variety 'Barke', a high quality malting variety. Cloning sites: EcoRI (5'-end of cDNA) and XhoI (3'-end of cDNA). NOTE: Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter. Average insert size is 1 kb Sequence trimming: Vector sequences and sequence ends were trimmed from the 5'-and 3'-end until a 50 bp window contains less than two ambiguities. The maximum length was set to 700 bp
DNA
gtatccttccattttatatccctcggtgatggatgtacctttggcatcta
aagttcccttgccctccccttccaagcatgaacaatgcaacgtttatagt
cataagagctcatcgaagcatgcanatctcaatccccatgctattgatag
tgttctcggtatcattcttggaggtggtgcagggactagattgtatcccc
tgacgaagaagcgtgcaaagcctgcagtgccattgggtgccaactacagg
cttattgatattcctgtcagtaattgtctgaacagcaacatatcaaagat
ctatgtgcttacacagttcaactcagcttctcttaatcgtcatctctcac
gagcctatgggagcaacattggaggttacaagaatgaaggatttgttgaa
gtccttgctgcacagcanagcccagataaccctgactggttccagggtac
tgcagatgctgtaaggcagtacttgtggctattcgaggagcataatgtta
tggagtatctaattcttgctggagatcacctgtaccgaatggactatgaa
aagtttattcaggcacacagagaaacggatgctgatattactgttgctgc
cttgcccatggatgaggaacgtgcaactgcatttggccttatgaaaaatc
gatgaaaaaaggggag

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: AL508997
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