Query (optional)   in Class  

GrainGenes Sequence Report: AL508149

[Submit comment/correction]

Sequence
AL508149
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC154429
NCBI UniGene
Hv.23299
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'CDNA clone: FLbaf86n19, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Tissue
developing caryopsis (3.-15.DAP)
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
AL508149 Hordeum vulgare Barke developing caryopsis (3.-15.DAP) Hordeum vulgare subsp. vulgare cDNA clone HY07O20V 5', mRNA sequence.
Strain
lab_host XLOLR
vulgare
Clone
HY07O20V
Probe
[Bcl698ct1201cn4503]
{SpCl-96}
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Michalek W; Institute for Plant Genetics and Crop Plant Research; Corrensstr.3, D-06466 Gatersleben, Germany; Email: michalek@ipk-gatersleben.de, http:
Note: Vector: plasmid pBK-CMV; Site_1: EcoRI; Site_2: XhoI; mRNA was made from developing caryopsis (3.-15.DAP) of spring barley variety 'Barke', a high quality malting variety. Cloning sites: EcoRI (5'-end of cDNA) and XhoI (3'-end of cDNA). NOTE: Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter. Average insert size is 1 kb Sequence trimming: Vector sequences and sequence ends were trimmed from the 5'-and 3'-end until a 50 bp window contains less than two ambiguities. The maximum length was set to 700 bp
DNA
gcggaagtccgcggtgcgccttgtcgcgtgccggccgcgcttcccctcct
ctcctctcctctcctctctccatcaccgcgctgtgcccaccacacgactc
ctctcccccaccccaccgcacggacggaagcaggcaggcgagcagctctc
cagcgactcggcgaggatggaaatgctcttcagcagcctgttctgtgact
cagcgtcatcggagaacttttctggccatcccggtgttgagagatgccca
ttcctgaggaacatcaatggagccacaaccttttcattttcttctgcttt
gcctgtagctgcccggggaggcaagggtccgatctttgaggatggaccag
gctttgagtcggcattcaagcttttccatggacaggatgggatagtcccc
ctttcaggaagatcatatgtgcctgatgagaaccgcagtgagagcactga
tgtcaagcctgaacctgctctgccctttaatccattggctgctagagccg
ctaccataagtctatcatcatttggaccatttgggttcaacttctttaat
ggcaagggcaaaaagcagaacaagaagcccaacaatctagaccagtcaca
caagaagcccaacaagccaaatcagaattccatggaaacaaaaaggaggc
aacccaccgtcccacgaggcaatgagcgatgaatggctagaaaatgg

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.