GrainGenes Sequence Report: AL506811

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Sequence

AL506811

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'CDNA clone: FLbaf137p04, mRNA sequence'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Hordeum vulgare Barke developing caryopsis (3.-15.DAP)

Tissue

developing caryopsis (3.-15.DAP)

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

AL506811 Hordeum vulgare Barke developing caryopsis (3.-15.DAP) Hordeum vulgare subsp. vulgare cDNA clone HY04F01T 5', mRNA sequence.

Strain

lab_host XLOLR

vulgare

Clone

HY04F01T

Probe

[Bcl1274ct1838cn5711]

{SpCl-445}

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Michalek W; Institute for Plant Genetics and Crop Plant Research; Corrensstr.3, D-06466 Gatersleben, Germany; Email: michalek@ipk-gatersleben.de, http:

Note: Vector: plasmid pBK-CMV; Site_1: EcoRI; Site_2: XhoI; mRNA was made from developing caryopsis (3.-15.DAP) of spring barley variety 'Barke', a high quality malting variety. Cloning sites: EcoRI (5'-end of cDNA) and XhoI (3'-end of cDNA). NOTE: Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter. Average insert size is 1 kb Sequence trimming: Vector sequences and sequence ends were trimmed from the 5'-and 3'-end until a 50 bp window contains less than two ambiguities. The maximum length was set to 700 bp

DNA

gctttggcggnagctagctcagccgccgatttggacgacaccggcgagca
ggacctcaccggcagcagcgcatattatccaactttgcagattcccccta
caaagtccgaggtgggaagactgctccattatcacaaccctgtggatcag
ttgacactagtctttgggttgcaaaatctggacagtgccttctgaagttn
gacagtcattatcttgtcaacagtacattgccctgcaagatgccaaaaat
aaagaacctgtctgatgcatgtaaagtatcattttctccagatggaccca
tatcctgaagaaacactcgagagagttncgtgcac


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