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GrainGenes Sequence Report: AL505563

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Sequence	AL505563
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC164986
NCBI UniGene	Hv.22576
DB Remark	Locus Source: Hordeum vulgare subsp. vulgare UniGene title 'CDNA clone: FLbaf163l18, mRNA sequence'
Keyword	EST
Species	Hordeum vulgare subsp. vulgare
Cultivar	barke
Clone Library	Hordeum vulgare Barke roots
Tissue	roots
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	AL505563 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare cDNA clone HW08N17V 5', mRNA sequence.
Strain	lab_host XLOLR vulgare
Clone	HW08N17V
Probe	[Bcl115ct407cn1750] {SpCl-198} AL505563
Remark	DB_xref: taxon:112509 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Michalek W; Institute for Plant Genetics and Crop Plant Research; Corrensstr.3, D-06466 Gatersleben, Germany; Email: michalek@ipk-gatersleben.de, http: Note: Vector: plasmid pBK-CMV; Site_1: EcoRI; Site_2: XhoI; mRNA was made from roots of spring barley variety 'Barke', a high quality malting variety. Roots were grown for two days on filter paper at room temperature Cloning sites: EcoRI (5'-end of cDNA) and XhoI (3'-end of cDNA). NOTE: Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter. Average insert size is 1 kb Sequence trimming: Vector sequences and sequence ends were trimmed from the 5'-and 3'-end until a 50 bp window contains less than two ambiguities. The maximum length was set to 700 bp
DNA	gcaccgaccgatctcgatcatggaggatgagaggagcacccantcatacc agggagctgaggccgatcaggtggaggtgacggacaggggcctactcggc aacctcctcggcaagaagaaggaggaggaggacaagaagaaggaggaaga gctggtcaccggcatggagaaggtctccgtggaagagcccgaggttaagg aggatggcgagaagaaggagactctcttctccaagctgcaccgatccagc tccagctccagctcgtctagtgacgaggaggaggaggaggangtcatcca tgagaacggtgaggngatcaagaggaagaagaagaagggtctcaaggaga agctcaaggagaagctgcccggccacaaggacaaccgaggctgagcacgt gacgggcctacccgcaccgacggcccccgcgtctgttcagactcaccatg acaccgacttcgtcgtcgagaagatcgacggcgacgcgaaggcagaggcc acgcccggcagtgcccngaggaggagaagaaaggcttcttggaaaagatc aaggagaagctgnccggcggccacaaagaagcccggaggacgctgccgcg gngcccgttcacgcacgcttgcttccagccgcc

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