Query (optional)   in Class  

GrainGenes Sequence Report: AL504096

[Submit comment/correction]

Sequence
AL504096
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC179858
NCBI UniGene
Hv.6428
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001046247.1 Os02g0205200 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
barke
Clone Library
Hordeum vulgare Barke roots
Tissue
roots
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
AL504096 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare cDNA clone HW04D03V 5', mRNA sequence.
Strain
lab_host XLOLR
vulgare
Clone
HW04D03V
Probe
bast65B0303
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Michalek W; Institute for Plant Genetics and Crop Plant Research; Corrensstr.3, D-06466 Gatersleben, Germany; Email: michalek@ipk-gatersleben.de, http:
Note: Vector: plasmid pBK-CMV; Site_1: EcoRI; Site_2: XhoI; mRNA was made from roots of spring barley variety 'Barke', a high quality malting variety. Roots were grown for two days on filter paper at room temperature Cloning sites: EcoRI (5'-end of cDNA) and XhoI (3'-end of cDNA). NOTE: Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter. Average insert size is 1 kb Sequence trimming: Vector sequences and sequence ends were trimmed from the 5'-and 3'-end until a 50 bp window contains less than two ambiguities. The maximum length was set to 700 bp
DNA
antagaagggagaactaagtctcgattttttttttttttttttgcagtta
acgncttccaagttcctgacgaattccttcgtgcacattcaagctcaatg
agcagtttgagcgcccaagcagtgaacagctgcctaattaacacggatgc
agcagcagcagcaacaacaacgccgtgcacccagcacactgtatggccag
ctagctcagccttntaggcgcgctttgggtgccgcgcgcgcacgctgacg
actgcgacgtcgtcgatcaccggcccgcagagcgagctcatgtcgtcggt
cctggtgttgtagaatgagctgaagaagacgacgcgcgtgcgaggcgacg
tggcgcggaacgggagcacggcacgcttggcggcgccgccctgccccgcc
gactcgtacggcaccttggccgactccctgccggcgtaggcctccaccat
gagcgagccgcggcatgtgttgctggcgtcgcccacgctgaaggacagcg
cgtactgcttccccggcacggtgcggatcacctgcgcgatggcgctctcc
ctcccggccagcagctccacggcccgacgcccccgcggcaccgngaagtg
gtcgctgtcgatgtacttgacggccttgagcgactccaccatccaccccg
gcagcggcgagtgctcgtccaccgtccgcgacgggatc

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.