GrainGenes Sequence Report: AL503014

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Sequence

AL503014

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'CDNA clone: FLbaf127c21, mRNA sequence'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

barke

Clone Library

Hordeum vulgare Barke roots

Tissue

roots

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

AL503014 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare cDNA clone HW09H05u 3', mRNA sequence.

Strain

lab_host XLOLR

vulgare

Clone

HW09H05u

Probe

GBR459

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Michalek W; Institute for Plant Genetics and Crop Plant Research; Corrensstr.3, D-06466 Gatersleben, Germany; Email: michalek@ipk-gatersleben.de, http:

Note: Vector: plasmid pBK-CMV; Site_1: EcoRI; Site_2: XhoI; mRNA was made from roots of spring barley variety 'Barke', a high quality malting variety. Roots were grown for two days on filter paper at room temperature Cloning sites: EcoRI (5'-end of cDNA) and XhoI (3'-end of cDNA). NOTE: Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRI adapter. Average insert size is 1 kb Sequence trimming: Vector sequences and sequence ends were trimmed from the 5'-and 3'-end until a 50 bp window contains less than two ambiguities. The maximum length was set to 700 bp

DNA

tttttttttttttttttttagttaataatgcactatcttttttatctaat
caccaaccgaacagcacagtaccagactcacgatgcttaaaactccctga
taaacacgtcgatcactagcaacgatagggtgacaaaatcatgctacata
atacagacagaaagatgttcatggttcctccaggctggccatggaaatgt
tctcagaacatagaagatgtaacctcagcgacttcagttacaacctgctt
gtattccgnttccagtttctccttcacctcttcagtgatctcccccttgg
ctggcttggtcatcaccaacacgcagcaggtgggcctcttggtantaccc
gcggtcgcaagctcctcttttgatgtcacatantatgtatgggacattgg
tcttcctcacaatgtatgggaagatg


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